Slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor.

While the slipknot topology in proteins has been known for over a decade, its evolutionary origin is still a mystery. We have identified a previously overlooked slipknot motif in a family of two-domain membrane transporters. Moreover, we found that these proteins are homologous to several families o...

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Autores principales:	Vasilina Zayats, Agata P Perlinska, Aleksandra I Jarmolinska, Borys Jastrzebski, Stanislaw Dunin-Horkawicz, Joanna I Sulkowska
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f105ce84f84c4826ad5083eca7f3d2ec
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spelling	oai:doaj.org-article:f105ce84f84c4826ad5083eca7f3d2ec2021-12-02T19:57:29ZSlipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor.1553-734X1553-735810.1371/journal.pcbi.1009502https://doaj.org/article/f105ce84f84c4826ad5083eca7f3d2ec2021-10-01T00:00:00Zhttps://doi.org/10.1371/journal.pcbi.1009502https://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358While the slipknot topology in proteins has been known for over a decade, its evolutionary origin is still a mystery. We have identified a previously overlooked slipknot motif in a family of two-domain membrane transporters. Moreover, we found that these proteins are homologous to several families of unknotted membrane proteins. This allows us to directly investigate the evolution of the slipknot motif. Based on our comprehensive analysis of 17 distantly related protein families, we have found that slipknotted and unknotted proteins share a common structural motif. Furthermore, this motif is conserved on the sequential level as well. Our results suggest that, regardless of topology, the proteins we studied evolved from a common unknotted ancestor single domain protein. Our phylogenetic analysis suggests the presence of at least seven parallel evolutionary scenarios that led to the current diversity of proteins in question. The tools we have developed in the process can now be used to investigate the evolution of other repeated-domain proteins.Vasilina ZayatsAgata P PerlinskaAleksandra I JarmolinskaBorys JastrzebskiStanislaw Dunin-HorkawiczJoanna I SulkowskaPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 17, Iss 10, p e1009502 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Vasilina Zayats Agata P Perlinska Aleksandra I Jarmolinska Borys Jastrzebski Stanislaw Dunin-Horkawicz Joanna I Sulkowska Slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor.
description	While the slipknot topology in proteins has been known for over a decade, its evolutionary origin is still a mystery. We have identified a previously overlooked slipknot motif in a family of two-domain membrane transporters. Moreover, we found that these proteins are homologous to several families of unknotted membrane proteins. This allows us to directly investigate the evolution of the slipknot motif. Based on our comprehensive analysis of 17 distantly related protein families, we have found that slipknotted and unknotted proteins share a common structural motif. Furthermore, this motif is conserved on the sequential level as well. Our results suggest that, regardless of topology, the proteins we studied evolved from a common unknotted ancestor single domain protein. Our phylogenetic analysis suggests the presence of at least seven parallel evolutionary scenarios that led to the current diversity of proteins in question. The tools we have developed in the process can now be used to investigate the evolution of other repeated-domain proteins.
format	article
author	Vasilina Zayats Agata P Perlinska Aleksandra I Jarmolinska Borys Jastrzebski Stanislaw Dunin-Horkawicz Joanna I Sulkowska
author_facet	Vasilina Zayats Agata P Perlinska Aleksandra I Jarmolinska Borys Jastrzebski Stanislaw Dunin-Horkawicz Joanna I Sulkowska
author_sort	Vasilina Zayats
title	Slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor.
title_short	Slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor.
title_full	Slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor.
title_fullStr	Slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor.
title_full_unstemmed	Slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor.
title_sort	slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f105ce84f84c4826ad5083eca7f3d2ec
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Slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor.

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