Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat

Abstract Meat from wildlife species (bushmeat) represents a major source of dietary protein in low- and middle-income countries where humans and wildlife live in close proximity. Despite the occurrence of zoonotic pathogens in wildlife, their prevalence in bushmeat remains unknown. To assess the ris...

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Autores principales:	Robab Katani, Megan A. Schilling, Beatus Lyimo, Ernest Eblate, Andimile Martin, Triza Tonui, Isabella M. Cattadori, Stephen C. Francesconi, Anna B. Estes, Dennis Rentsch, Sreenidhi Srinivasan, Samson Lyimo, Lidia Munuo, Christian K. Tiambo, Francesca Stomeo, Paul Gwakisa, Fausta Mosha, Peter J. Hudson, Joram J. Buza, Vivek Kapur
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f14e2af474374f319b89f6513065bac3
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spelling	oai:doaj.org-article:f14e2af474374f319b89f6513065bac32021-12-02T16:17:21ZIdentification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat10.1038/s41598-021-94112-92045-2322https://doaj.org/article/f14e2af474374f319b89f6513065bac32021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94112-9https://doaj.org/toc/2045-2322Abstract Meat from wildlife species (bushmeat) represents a major source of dietary protein in low- and middle-income countries where humans and wildlife live in close proximity. Despite the occurrence of zoonotic pathogens in wildlife, their prevalence in bushmeat remains unknown. To assess the risk of exposure to major pathogens in bushmeat, a total of 3784 samples, both fresh and processed, were collected from three major regions in Tanzania during both rainy and dry seasons, and were screened by real-time PCR for the presence of DNA signatures of Bacillus anthracis (B. anthracis), Brucella spp. (Brucella) and Coxiella burnetii (Coxiella). The analysis identified DNA signatures of B. anthracis (0.48%), Brucella (0.9%), and Coxiella (0.66%) in a total of 77 samples. Highest prevalence rates of B. anthracis, Brucella, and Coxiella were observed in wildebeest (56%), dik-dik (50%), and impala (24%), respectively. Fresh samples, those collected during the rainy season, and samples from Selous or Serengeti had a greater relative risk of being positive. Microbiome characterization identified Firmicutes and Proteobacteria as the most abundant phyla. The results highlight and define potential risks of exposure to endemic wildlife diseases from bushmeat and the need for future investigations to address the public health and emerging infectious disease risks associated with bushmeat harvesting, trade, and consumption.Robab KataniMegan A. SchillingBeatus LyimoErnest EblateAndimile MartinTriza TonuiIsabella M. CattadoriStephen C. FrancesconiAnna B. EstesDennis RentschSreenidhi SrinivasanSamson LyimoLidia MunuoChristian K. TiamboFrancesca StomeoPaul GwakisaFausta MoshaPeter J. HudsonJoram J. BuzaVivek KapurNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Robab Katani Megan A. Schilling Beatus Lyimo Ernest Eblate Andimile Martin Triza Tonui Isabella M. Cattadori Stephen C. Francesconi Anna B. Estes Dennis Rentsch Sreenidhi Srinivasan Samson Lyimo Lidia Munuo Christian K. Tiambo Francesca Stomeo Paul Gwakisa Fausta Mosha Peter J. Hudson Joram J. Buza Vivek Kapur Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
description	Abstract Meat from wildlife species (bushmeat) represents a major source of dietary protein in low- and middle-income countries where humans and wildlife live in close proximity. Despite the occurrence of zoonotic pathogens in wildlife, their prevalence in bushmeat remains unknown. To assess the risk of exposure to major pathogens in bushmeat, a total of 3784 samples, both fresh and processed, were collected from three major regions in Tanzania during both rainy and dry seasons, and were screened by real-time PCR for the presence of DNA signatures of Bacillus anthracis (B. anthracis), Brucella spp. (Brucella) and Coxiella burnetii (Coxiella). The analysis identified DNA signatures of B. anthracis (0.48%), Brucella (0.9%), and Coxiella (0.66%) in a total of 77 samples. Highest prevalence rates of B. anthracis, Brucella, and Coxiella were observed in wildebeest (56%), dik-dik (50%), and impala (24%), respectively. Fresh samples, those collected during the rainy season, and samples from Selous or Serengeti had a greater relative risk of being positive. Microbiome characterization identified Firmicutes and Proteobacteria as the most abundant phyla. The results highlight and define potential risks of exposure to endemic wildlife diseases from bushmeat and the need for future investigations to address the public health and emerging infectious disease risks associated with bushmeat harvesting, trade, and consumption.
format	article
author	Robab Katani Megan A. Schilling Beatus Lyimo Ernest Eblate Andimile Martin Triza Tonui Isabella M. Cattadori Stephen C. Francesconi Anna B. Estes Dennis Rentsch Sreenidhi Srinivasan Samson Lyimo Lidia Munuo Christian K. Tiambo Francesca Stomeo Paul Gwakisa Fausta Mosha Peter J. Hudson Joram J. Buza Vivek Kapur
author_facet	Robab Katani Megan A. Schilling Beatus Lyimo Ernest Eblate Andimile Martin Triza Tonui Isabella M. Cattadori Stephen C. Francesconi Anna B. Estes Dennis Rentsch Sreenidhi Srinivasan Samson Lyimo Lidia Munuo Christian K. Tiambo Francesca Stomeo Paul Gwakisa Fausta Mosha Peter J. Hudson Joram J. Buza Vivek Kapur
author_sort	Robab Katani
title	Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
title_short	Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
title_full	Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
title_fullStr	Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
title_full_unstemmed	Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
title_sort	identification of bacillus anthracis, brucella spp., and coxiella burnetii dna signatures from bushmeat
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f14e2af474374f319b89f6513065bac3
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Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat

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