A proteomic view at T cell costimulation.

The "two-signal paradigm" in T cell activation predicts that the cooperation of "signal 1," provided by the T cell receptor (TCR) through engagement of major histocompatility complex (MHC)-presented peptide, with "signal 2″ provided by costimulatory molecules, the prototype...

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Autores principales:	Rudolf Lichtenfels, Gunter Rappl, Andreas A Hombach, Christian V Recktenwald, Sven P Dressler, Hinrich Abken, Barbara Seliger
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f15381e356fe4abd9692c874411232ca
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spelling	oai:doaj.org-article:f15381e356fe4abd9692c874411232ca2021-11-18T07:21:20ZA proteomic view at T cell costimulation.1932-620310.1371/journal.pone.0032994https://doaj.org/article/f15381e356fe4abd9692c874411232ca2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22539942/?tool=EBIhttps://doaj.org/toc/1932-6203The "two-signal paradigm" in T cell activation predicts that the cooperation of "signal 1," provided by the T cell receptor (TCR) through engagement of major histocompatility complex (MHC)-presented peptide, with "signal 2″ provided by costimulatory molecules, the prototype of which is CD28, is required to induce T cell effector functions. While the individual signalling pathways are well understood, little is known about global changes in the proteome pattern during TCR/CD28-mediated activation. Therefore, comparative 2-DE-based proteome analyses of CD3(+) CD69(-) resting T cells versus cells incubated with (i) the agonistic anti-CD3 antibody OKT3 mimicking signal 1 in absence or presence of IL-2 and/or with (ii) the agonistic antibody 15E8 triggering CD28-mediated signaling were performed. Differentially regulated spots were defined leading to the identification of proteins involved in the regulation of the metabolism, shaping and maintenance of the cytoskeleton and signal transduction. Representative members of the differentially expressed protein families, such as calmodulin (CALM), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), L-lactate dehydrogenase (LDH), Rho GDP-dissociation inhibitor 2 (GDIR2), and platelet basic protein (CXCL7), were independently verified by flow cytometry. Data provide a detailed map of individual protein alterations at the global proteome level in response to TCR/CD28-mediated T cell activation.Rudolf LichtenfelsGunter RapplAndreas A HombachChristian V RecktenwaldSven P DresslerHinrich AbkenBarbara SeligerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e32994 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rudolf Lichtenfels Gunter Rappl Andreas A Hombach Christian V Recktenwald Sven P Dressler Hinrich Abken Barbara Seliger A proteomic view at T cell costimulation.
description	The "two-signal paradigm" in T cell activation predicts that the cooperation of "signal 1," provided by the T cell receptor (TCR) through engagement of major histocompatility complex (MHC)-presented peptide, with "signal 2″ provided by costimulatory molecules, the prototype of which is CD28, is required to induce T cell effector functions. While the individual signalling pathways are well understood, little is known about global changes in the proteome pattern during TCR/CD28-mediated activation. Therefore, comparative 2-DE-based proteome analyses of CD3(+) CD69(-) resting T cells versus cells incubated with (i) the agonistic anti-CD3 antibody OKT3 mimicking signal 1 in absence or presence of IL-2 and/or with (ii) the agonistic antibody 15E8 triggering CD28-mediated signaling were performed. Differentially regulated spots were defined leading to the identification of proteins involved in the regulation of the metabolism, shaping and maintenance of the cytoskeleton and signal transduction. Representative members of the differentially expressed protein families, such as calmodulin (CALM), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), L-lactate dehydrogenase (LDH), Rho GDP-dissociation inhibitor 2 (GDIR2), and platelet basic protein (CXCL7), were independently verified by flow cytometry. Data provide a detailed map of individual protein alterations at the global proteome level in response to TCR/CD28-mediated T cell activation.
format	article
author	Rudolf Lichtenfels Gunter Rappl Andreas A Hombach Christian V Recktenwald Sven P Dressler Hinrich Abken Barbara Seliger
author_facet	Rudolf Lichtenfels Gunter Rappl Andreas A Hombach Christian V Recktenwald Sven P Dressler Hinrich Abken Barbara Seliger
author_sort	Rudolf Lichtenfels
title	A proteomic view at T cell costimulation.
title_short	A proteomic view at T cell costimulation.
title_full	A proteomic view at T cell costimulation.
title_fullStr	A proteomic view at T cell costimulation.
title_full_unstemmed	A proteomic view at T cell costimulation.
title_sort	proteomic view at t cell costimulation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f15381e356fe4abd9692c874411232ca
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A proteomic view at T cell costimulation.

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