Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit

Abstract. Hidayat H, Haryadi W, Matsjeh S, Raharjo TJ. 2019. Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit. Biodiversitas 20: 2222-2228. Various fermentation food involves microorganisms, especially Lactic A...

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Autores principales:	Habibi Hidayat, WINARTO HARYADI, SABIRIN MATSJEH, TRI JOKO RAHARJO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	active proteolytic, lactic acid bacteria, kelengkeng, 16s rrna gene Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f18b10e6a3e34c28ac0abe35fc4c51ac
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spelling	oai:doaj.org-article:f18b10e6a3e34c28ac0abe35fc4c51ac2021-11-21T21:43:55ZMolecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit1412-033X2085-472210.13057/biodiv/d200816https://doaj.org/article/f18b10e6a3e34c28ac0abe35fc4c51ac2019-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3697https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Hidayat H, Haryadi W, Matsjeh S, Raharjo TJ. 2019. Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit. Biodiversitas 20: 2222-2228. Various fermentation food involves microorganisms, especially Lactic Acid Bacteria (LAB) which has beneficial properties for fermented food. The present study aims to do identification and characterization of active proteolytic LAB from kelengkeng (Dimocarpus longan), a commonly found fruit in South East Asia. From ten LAB isolates, isolates K7 and K8 appeared as basil shape based on Gram staining and were chosen for further examination. The molecular identification of these selected isolates were investigated from the 16S rRNA analysis using universal primer 27F and 1525R. The K7 and K8 isolates showed resistance ability towards acidic environment (pH 2.0) and were examined for protease enzyme screening resulting 7.5 and 6 to clear zone, which was 17 and 12 mm. The result of the molecular characterization from the two isolates indicated that isolate K7 was identified as Leuconostoc mesenteroides subsp suionicum strain LT-38 while isolate K8 was identified as Leuconostoc mesenteroides strain C305.16.Habibi HidayatWINARTO HARYADISABIRIN MATSJEHTRI JOKO RAHARJOMBI & UNS Soloarticleactive proteolytic, lactic acid bacteria, kelengkeng, 16s rrna geneBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	active proteolytic, lactic acid bacteria, kelengkeng, 16s rrna gene Biology (General) QH301-705.5
spellingShingle	active proteolytic, lactic acid bacteria, kelengkeng, 16s rrna gene Biology (General) QH301-705.5 Habibi Hidayat WINARTO HARYADI SABIRIN MATSJEH TRI JOKO RAHARJO Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit
description	Abstract. Hidayat H, Haryadi W, Matsjeh S, Raharjo TJ. 2019. Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit. Biodiversitas 20: 2222-2228. Various fermentation food involves microorganisms, especially Lactic Acid Bacteria (LAB) which has beneficial properties for fermented food. The present study aims to do identification and characterization of active proteolytic LAB from kelengkeng (Dimocarpus longan), a commonly found fruit in South East Asia. From ten LAB isolates, isolates K7 and K8 appeared as basil shape based on Gram staining and were chosen for further examination. The molecular identification of these selected isolates were investigated from the 16S rRNA analysis using universal primer 27F and 1525R. The K7 and K8 isolates showed resistance ability towards acidic environment (pH 2.0) and were examined for protease enzyme screening resulting 7.5 and 6 to clear zone, which was 17 and 12 mm. The result of the molecular characterization from the two isolates indicated that isolate K7 was identified as Leuconostoc mesenteroides subsp suionicum strain LT-38 while isolate K8 was identified as Leuconostoc mesenteroides strain C305.16.
format	article
author	Habibi Hidayat WINARTO HARYADI SABIRIN MATSJEH TRI JOKO RAHARJO
author_facet	Habibi Hidayat WINARTO HARYADI SABIRIN MATSJEH TRI JOKO RAHARJO
author_sort	Habibi Hidayat
title	Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit
title_short	Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit
title_full	Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit
title_fullStr	Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit
title_full_unstemmed	Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit
title_sort	molecular identification 16s rrna gene of active proteolytic lactic acid bacteria (lab) isolated from kelengkeng (dimocarpus longan) fruit
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/f18b10e6a3e34c28ac0abe35fc4c51ac
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Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit

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