Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.
Reciprocal interactions between neurons and oligodendrocytes are not only crucial for myelination, but also for long-term survival of axons. Degeneration of axons occurs in several human myelin diseases, however the molecular mechanisms of axon-glia communication maintaining axon integrity are poorl...
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2013
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oai:doaj.org-article:f195507aa8304bdba87e81a5638558aa2021-11-18T05:37:00ZNeurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.1544-91731545-788510.1371/journal.pbio.1001604https://doaj.org/article/f195507aa8304bdba87e81a5638558aa2013-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874151/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Reciprocal interactions between neurons and oligodendrocytes are not only crucial for myelination, but also for long-term survival of axons. Degeneration of axons occurs in several human myelin diseases, however the molecular mechanisms of axon-glia communication maintaining axon integrity are poorly understood. Here, we describe the signal-mediated transfer of exosomes from oligodendrocytes to neurons. These endosome-derived vesicles are secreted by oligodendrocytes and carry specific protein and RNA cargo. We show that activity-dependent release of the neurotransmitter glutamate triggers oligodendroglial exosome secretion mediated by Ca²⁺ entry through oligodendroglial NMDA and AMPA receptors. In turn, neurons internalize the released exosomes by endocytosis. Injection of oligodendroglia-derived exosomes into the mouse brain results in functional retrieval of exosome cargo in neurons. Supply of cultured neurons with oligodendroglial exosomes improves neuronal viability under conditions of cell stress. These findings indicate that oligodendroglial exosomes participate in a novel mode of bidirectional neuron-glia communication contributing to neuronal integrity.Carsten FrühbeisDominik FröhlichWen Ping KuoJesa AmphornratSebastian ThilemannAiman S SaabFrank KirchhoffWiebke MöbiusSandra GoebbelsKlaus-Armin NaveAnja SchneiderMikael SimonsMatthias KlugmannJacqueline TrotterEva-Maria Krämer-AlbersPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 11, Iss 7, p e1001604 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Biology (General) QH301-705.5 |
| spellingShingle |
Biology (General) QH301-705.5 Carsten Frühbeis Dominik Fröhlich Wen Ping Kuo Jesa Amphornrat Sebastian Thilemann Aiman S Saab Frank Kirchhoff Wiebke Möbius Sandra Goebbels Klaus-Armin Nave Anja Schneider Mikael Simons Matthias Klugmann Jacqueline Trotter Eva-Maria Krämer-Albers Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication. |
| description |
Reciprocal interactions between neurons and oligodendrocytes are not only crucial for myelination, but also for long-term survival of axons. Degeneration of axons occurs in several human myelin diseases, however the molecular mechanisms of axon-glia communication maintaining axon integrity are poorly understood. Here, we describe the signal-mediated transfer of exosomes from oligodendrocytes to neurons. These endosome-derived vesicles are secreted by oligodendrocytes and carry specific protein and RNA cargo. We show that activity-dependent release of the neurotransmitter glutamate triggers oligodendroglial exosome secretion mediated by Ca²⁺ entry through oligodendroglial NMDA and AMPA receptors. In turn, neurons internalize the released exosomes by endocytosis. Injection of oligodendroglia-derived exosomes into the mouse brain results in functional retrieval of exosome cargo in neurons. Supply of cultured neurons with oligodendroglial exosomes improves neuronal viability under conditions of cell stress. These findings indicate that oligodendroglial exosomes participate in a novel mode of bidirectional neuron-glia communication contributing to neuronal integrity. |
| format |
article |
| author |
Carsten Frühbeis Dominik Fröhlich Wen Ping Kuo Jesa Amphornrat Sebastian Thilemann Aiman S Saab Frank Kirchhoff Wiebke Möbius Sandra Goebbels Klaus-Armin Nave Anja Schneider Mikael Simons Matthias Klugmann Jacqueline Trotter Eva-Maria Krämer-Albers |
| author_facet |
Carsten Frühbeis Dominik Fröhlich Wen Ping Kuo Jesa Amphornrat Sebastian Thilemann Aiman S Saab Frank Kirchhoff Wiebke Möbius Sandra Goebbels Klaus-Armin Nave Anja Schneider Mikael Simons Matthias Klugmann Jacqueline Trotter Eva-Maria Krämer-Albers |
| author_sort |
Carsten Frühbeis |
| title |
Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication. |
| title_short |
Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication. |
| title_full |
Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication. |
| title_fullStr |
Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication. |
| title_full_unstemmed |
Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication. |
| title_sort |
neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/f195507aa8304bdba87e81a5638558aa |
| work_keys_str_mv |
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