Comparing gut resistome composition among patients with acute Campylobacter infections and healthy family members

Abstract Campylobacter commonly causes foodborne infections and antibiotic resistance is an imminent concern. It is not clear, however, if the human gut ‘resistome’ is affected by Campylobacter during infection. Application of shotgun metagenomics on stools from 26 cases with Campylobacter infection...

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Autores principales:	Zoe A. Hansen, Wonhee Cha, Brian Nohomovich, Duane W. Newton, Paul Lephart, Hossein Salimnia, Walid Khalife, Ashley Shade, James T. Rudrik, Shannon D. Manning
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f1afed909e8e45ce8dcd4f7d50db923a
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spelling	oai:doaj.org-article:f1afed909e8e45ce8dcd4f7d50db923a2021-11-21T12:16:24ZComparing gut resistome composition among patients with acute Campylobacter infections and healthy family members10.1038/s41598-021-01927-72045-2322https://doaj.org/article/f1afed909e8e45ce8dcd4f7d50db923a2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01927-7https://doaj.org/toc/2045-2322Abstract Campylobacter commonly causes foodborne infections and antibiotic resistance is an imminent concern. It is not clear, however, if the human gut ‘resistome’ is affected by Campylobacter during infection. Application of shotgun metagenomics on stools from 26 cases with Campylobacter infections and 44 healthy family members (controls) identified 406 unique antibiotic resistance genes (ARGs) representing 153 genes/operons, 40 mechanisms, and 18 classes. Cases had greater ARG richness (p < 0.0001) and Shannon diversity (p < 0.0001) than controls with distinct compositions (p = 0.000999; PERMANOVA). Cases were defined by multidrug resistance genes and were dominated by Proteobacteria (40.8%), specifically those representing Escherichia (20.9%). Tetracycline resistance genes were most abundant in controls, which were dominated by Bacteroidetes (45.3%) and Firmicutes (44.4%). Hierarchical clustering of cases identified three clusters with distinct resistomes. Case clusters 1 and 3 differed from controls containing more urban and hospitalized patients. Relative to family members of the same household, ARG composition among matched cases was mostly distinct, though some familial controls had similar profiles that could be explained by a shorter time since exposure to the case. Together, these data indicate that Campylobacter infection is associated with an altered resistome composition and increased ARG diversity, raising concerns about the role of infection in the spread of resistance determinants.Zoe A. HansenWonhee ChaBrian NohomovichDuane W. NewtonPaul LephartHossein SalimniaWalid KhalifeAshley ShadeJames T. RudrikShannon D. ManningNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zoe A. Hansen Wonhee Cha Brian Nohomovich Duane W. Newton Paul Lephart Hossein Salimnia Walid Khalife Ashley Shade James T. Rudrik Shannon D. Manning Comparing gut resistome composition among patients with acute Campylobacter infections and healthy family members
description	Abstract Campylobacter commonly causes foodborne infections and antibiotic resistance is an imminent concern. It is not clear, however, if the human gut ‘resistome’ is affected by Campylobacter during infection. Application of shotgun metagenomics on stools from 26 cases with Campylobacter infections and 44 healthy family members (controls) identified 406 unique antibiotic resistance genes (ARGs) representing 153 genes/operons, 40 mechanisms, and 18 classes. Cases had greater ARG richness (p < 0.0001) and Shannon diversity (p < 0.0001) than controls with distinct compositions (p = 0.000999; PERMANOVA). Cases were defined by multidrug resistance genes and were dominated by Proteobacteria (40.8%), specifically those representing Escherichia (20.9%). Tetracycline resistance genes were most abundant in controls, which were dominated by Bacteroidetes (45.3%) and Firmicutes (44.4%). Hierarchical clustering of cases identified three clusters with distinct resistomes. Case clusters 1 and 3 differed from controls containing more urban and hospitalized patients. Relative to family members of the same household, ARG composition among matched cases was mostly distinct, though some familial controls had similar profiles that could be explained by a shorter time since exposure to the case. Together, these data indicate that Campylobacter infection is associated with an altered resistome composition and increased ARG diversity, raising concerns about the role of infection in the spread of resistance determinants.
format	article
author	Zoe A. Hansen Wonhee Cha Brian Nohomovich Duane W. Newton Paul Lephart Hossein Salimnia Walid Khalife Ashley Shade James T. Rudrik Shannon D. Manning
author_facet	Zoe A. Hansen Wonhee Cha Brian Nohomovich Duane W. Newton Paul Lephart Hossein Salimnia Walid Khalife Ashley Shade James T. Rudrik Shannon D. Manning
author_sort	Zoe A. Hansen
title	Comparing gut resistome composition among patients with acute Campylobacter infections and healthy family members
title_short	Comparing gut resistome composition among patients with acute Campylobacter infections and healthy family members
title_full	Comparing gut resistome composition among patients with acute Campylobacter infections and healthy family members
title_fullStr	Comparing gut resistome composition among patients with acute Campylobacter infections and healthy family members
title_full_unstemmed	Comparing gut resistome composition among patients with acute Campylobacter infections and healthy family members
title_sort	comparing gut resistome composition among patients with acute campylobacter infections and healthy family members
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f1afed909e8e45ce8dcd4f7d50db923a
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Comparing gut resistome composition among patients with acute Campylobacter infections and healthy family members

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