Single-cell measurement of plasmid copy number and promoter activity

A quantitative assessment of promoter function can improve the precision of cellular engineering. Here the authors develop a method to simultaneously count plasmid DNA, RNA transcripts and protein expression in single living bacteria.

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Auteurs principaux: Bin Shao, Jayan Rammohan, Daniel A. Anderson, Nina Alperovich, David Ross, Christopher A. Voigt
Format: article
Langue:EN
Publié: Nature Portfolio 2021
Sujets:
Q
Accès en ligne:https://doaj.org/article/f213c242413e488095465d237f992e7a
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