Single-cell measurement of plasmid copy number and promoter activity

A quantitative assessment of promoter function can improve the precision of cellular engineering. Here the authors develop a method to simultaneously count plasmid DNA, RNA transcripts and protein expression in single living bacteria.

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Detalles Bibliográficos
Autores principales:	Bin Shao, Jayan Rammohan, Daniel A. Anderson, Nina Alperovich, David Ross, Christopher A. Voigt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/f213c242413e488095465d237f992e7a
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spelling	oai:doaj.org-article:f213c242413e488095465d237f992e7a2021-12-02T13:32:54ZSingle-cell measurement of plasmid copy number and promoter activity10.1038/s41467-021-21734-y2041-1723https://doaj.org/article/f213c242413e488095465d237f992e7a2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21734-yhttps://doaj.org/toc/2041-1723A quantitative assessment of promoter function can improve the precision of cellular engineering. Here the authors develop a method to simultaneously count plasmid DNA, RNA transcripts and protein expression in single living bacteria.Bin ShaoJayan RammohanDaniel A. AndersonNina AlperovichDavid RossChristopher A. VoigtNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Bin Shao Jayan Rammohan Daniel A. Anderson Nina Alperovich David Ross Christopher A. Voigt Single-cell measurement of plasmid copy number and promoter activity
description	A quantitative assessment of promoter function can improve the precision of cellular engineering. Here the authors develop a method to simultaneously count plasmid DNA, RNA transcripts and protein expression in single living bacteria.
format	article
author	Bin Shao Jayan Rammohan Daniel A. Anderson Nina Alperovich David Ross Christopher A. Voigt
author_facet	Bin Shao Jayan Rammohan Daniel A. Anderson Nina Alperovich David Ross Christopher A. Voigt
author_sort	Bin Shao
title	Single-cell measurement of plasmid copy number and promoter activity
title_short	Single-cell measurement of plasmid copy number and promoter activity
title_full	Single-cell measurement of plasmid copy number and promoter activity
title_fullStr	Single-cell measurement of plasmid copy number and promoter activity
title_full_unstemmed	Single-cell measurement of plasmid copy number and promoter activity
title_sort	single-cell measurement of plasmid copy number and promoter activity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f213c242413e488095465d237f992e7a
work_keys_str_mv	AT binshao singlecellmeasurementofplasmidcopynumberandpromoteractivity AT jayanrammohan singlecellmeasurementofplasmidcopynumberandpromoteractivity AT danielaanderson singlecellmeasurementofplasmidcopynumberandpromoteractivity AT ninaalperovich singlecellmeasurementofplasmidcopynumberandpromoteractivity AT davidross singlecellmeasurementofplasmidcopynumberandpromoteractivity AT christopheravoigt singlecellmeasurementofplasmidcopynumberandpromoteractivity
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Single-cell measurement of plasmid copy number and promoter activity

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