Proliferative effect of Hachimijiogan, a Japanese herbal medicine, in C2C12 skeletal muscle cells

Takashi Takeda,1,2 Kenji Tsuiji,2 Bin Li,2 Mari Tadakawa,2 Nobuo Yaegashi2 1Division of Women’s Health, Research Institute of Traditional Asian Medicine, Kinki University School of Medicine, Osaka, Japan; 2Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medic...

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Autores principales:	Takeda T, Tsuiji K, Li B, Tadakawa M, Yaegashi N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	herbal medicine sarcopenia myoblast ERK1/2 signaling pathway proliferation Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/f28492a8cb054366a3057d7125903860
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spelling	oai:doaj.org-article:f28492a8cb054366a3057d71259038602021-12-02T01:30:16ZProliferative effect of Hachimijiogan, a Japanese herbal medicine, in C2C12 skeletal muscle cells1178-1998https://doaj.org/article/f28492a8cb054366a3057d71259038602015-02-01T00:00:00Zhttps://www.dovepress.com/proliferative-effect-of-hachimijiogan-a-japanese-herbal-medicine-in-c2-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Takashi Takeda,1,2 Kenji Tsuiji,2 Bin Li,2 Mari Tadakawa,2 Nobuo Yaegashi2 1Division of Women’s Health, Research Institute of Traditional Asian Medicine, Kinki University School of Medicine, Osaka, Japan; 2Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Sendai, Japan Background: Hachimijiogan (HJG), Ba-Wei-Di-Huang-Wan in Chinese, is one of the most popular herbal medicines in Japanese Kampo. HJG is often prescribed for the prevention and treatment of age-related diseases. Muscle atrophy plays an important role in aging-related disabilities such as sarcopenia. The purpose of this study was to investigate the possible beneficial effect of HJG on skeletal muscle.Methods: Cells of murine skeletal muscle myoblast cell line C2C12 were used as an in vitro model of muscle cell proliferation and differentiation. The effect of HJG on C2C12 cell proliferation and differentiation was assessed. We counted the number of myotubes morphologically to assess the degree of differentiation.Results: HJG treatment (200 µg/mL) for 3 days significantly increased C2C12 cell number by 1.23-fold compared with that of the control. HJG promoted the proliferation of C2C12 cells through activation of the ERK1/2 signaling pathway without affecting the Akt signaling pathway. HJG did not affect the differentiation of C2C12 cells. Conclusion: HJG had beneficial effects on skeletal muscle myoblast proliferation. These findings may provide a useful intervention for the prevention and treatment of sarcopenia. Keywords: ERK1/2 signaling pathway, herbal medicine, myoblast, proliferation, sarcopeniaTakeda TTsuiji KLi BTadakawa MYaegashi NDove Medical Pressarticleherbal medicinesarcopeniamyoblastERK1/2 signaling pathwayproliferationGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 10, Pp 445-451 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	herbal medicine sarcopenia myoblast ERK1/2 signaling pathway proliferation Geriatrics RC952-954.6
spellingShingle	herbal medicine sarcopenia myoblast ERK1/2 signaling pathway proliferation Geriatrics RC952-954.6 Takeda T Tsuiji K Li B Tadakawa M Yaegashi N Proliferative effect of Hachimijiogan, a Japanese herbal medicine, in C2C12 skeletal muscle cells
description	Takashi Takeda,1,2 Kenji Tsuiji,2 Bin Li,2 Mari Tadakawa,2 Nobuo Yaegashi2 1Division of Women’s Health, Research Institute of Traditional Asian Medicine, Kinki University School of Medicine, Osaka, Japan; 2Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Sendai, Japan Background: Hachimijiogan (HJG), Ba-Wei-Di-Huang-Wan in Chinese, is one of the most popular herbal medicines in Japanese Kampo. HJG is often prescribed for the prevention and treatment of age-related diseases. Muscle atrophy plays an important role in aging-related disabilities such as sarcopenia. The purpose of this study was to investigate the possible beneficial effect of HJG on skeletal muscle.Methods: Cells of murine skeletal muscle myoblast cell line C2C12 were used as an in vitro model of muscle cell proliferation and differentiation. The effect of HJG on C2C12 cell proliferation and differentiation was assessed. We counted the number of myotubes morphologically to assess the degree of differentiation.Results: HJG treatment (200 µg/mL) for 3 days significantly increased C2C12 cell number by 1.23-fold compared with that of the control. HJG promoted the proliferation of C2C12 cells through activation of the ERK1/2 signaling pathway without affecting the Akt signaling pathway. HJG did not affect the differentiation of C2C12 cells. Conclusion: HJG had beneficial effects on skeletal muscle myoblast proliferation. These findings may provide a useful intervention for the prevention and treatment of sarcopenia. Keywords: ERK1/2 signaling pathway, herbal medicine, myoblast, proliferation, sarcopenia
format	article
author	Takeda T Tsuiji K Li B Tadakawa M Yaegashi N
author_facet	Takeda T Tsuiji K Li B Tadakawa M Yaegashi N
author_sort	Takeda T
title	Proliferative effect of Hachimijiogan, a Japanese herbal medicine, in C2C12 skeletal muscle cells
title_short	Proliferative effect of Hachimijiogan, a Japanese herbal medicine, in C2C12 skeletal muscle cells
title_full	Proliferative effect of Hachimijiogan, a Japanese herbal medicine, in C2C12 skeletal muscle cells
title_fullStr	Proliferative effect of Hachimijiogan, a Japanese herbal medicine, in C2C12 skeletal muscle cells
title_full_unstemmed	Proliferative effect of Hachimijiogan, a Japanese herbal medicine, in C2C12 skeletal muscle cells
title_sort	proliferative effect of hachimijiogan, a japanese herbal medicine, in c2c12 skeletal muscle cells
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/f28492a8cb054366a3057d7125903860
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Proliferative effect of Hachimijiogan, a Japanese herbal medicine, in C2C12 skeletal muscle cells

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