A Novel Protocol for the Isolation of Fungal Extracellular Vesicles Reveals the Participation of a Putative Scramblase in Polysaccharide Export and Capsule Construction in <italic toggle="yes">Cryptococcus gattii</italic>

A Novel Protocol for the Isolation of Fungal Extracellular Vesicles Reveals the Participation of a Putative Scramblase in Polysaccharide Export and Capsule Construction in <italic toggle="yes">Cryptococcus gattii</italic>

ABSTRACT Regular protocols for the isolation of fungal extracellular vesicles (EVs) are time-consuming, hard to reproduce, and produce low yields. In an attempt to improve the protocols used for EV isolation, we explored a model of vesicle production after growth of Cryptococcus gattii and Cryptococ...

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Bibliographic Details
Main Authors: Flavia C. G. Reis, Beatriz S. Borges, Luísa J. Jozefowicz, Bianca A. G. Sena, Ane W. A. Garcia, Lia C. Medeiros, Sharon T. Martins, Leandro Honorato, Augusto Schrank, Marilene H. Vainstein, Livia Kmetzsch, Leonardo Nimrichter, Lysangela R. Alves, Charley C. Staats, Marcio L. Rodrigues
Format: article
Language:EN
Published: American Society for Microbiology 2019
Subjects:
Cryptococcus
extracellular vesicles
fungi
secretion
scramblase
Microbiology
QR1-502
Online Access:https://doaj.org/article/f285e1929e83407b83579be30af37ba1
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https://doaj.org/article/f285e1929e83407b83579be30af37ba1

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