Rapid Screening and Estimation of Binding Constants for Interactions of Fe3+ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis

The interaction behavior of Fe3+ with transferrin and apotransferrin (iron-free form) was investigated in this study using affinity capillary electrophoresis. Change in the mass and charge of protein upon binding to the metal ion in the capillary tube led to variation in its migration time and was u...

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Auteurs principaux: Mohammed Al Bratty, Hassan A. Alhazmi, Sadique A. Javed, Zia Ur Rehman, Asim Najmi, Karam A. El-Sharkawy
Format: article
Langue:EN
Publié: Hindawi Limited 2021
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Accès en ligne:https://doaj.org/article/f30155d4630b4e149f317fdc1482a7df
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