Mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity

Abstract The pectin methylesterase action is usually studied in a homogeneous aqueous medium in the presence of a large excess of soluble substrate and water. However in the cell wall, the water content is much lower, the substrate is cross-linked with itself or with other polymers, and the enzyme h...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Estelle Bonnin, Camille Alvarado, Marie-Jeanne Crépeau, Brigitte Bouchet, Catherine Garnier, Frédéric Jamme, Marie-Françoise Devaux
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3235ae88af14d1caea39ad1936801df
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f3235ae88af14d1caea39ad1936801df
record_format	dspace
spelling	oai:doaj.org-article:f3235ae88af14d1caea39ad1936801df2021-12-02T15:07:55ZMobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity10.1038/s41598-019-49108-x2045-2322https://doaj.org/article/f3235ae88af14d1caea39ad1936801df2019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-49108-xhttps://doaj.org/toc/2045-2322Abstract The pectin methylesterase action is usually studied in a homogeneous aqueous medium in the presence of a large excess of soluble substrate and water. However in the cell wall, the water content is much lower, the substrate is cross-linked with itself or with other polymers, and the enzyme has to diffuse through the solid matrix before catalysing the linkage breakdown. As plant primary cell walls can be considered as cellulose-reinforced hydrogels, this study investigated the diffusion of a fungal pectin methylesterase in pectin/cellulose gels used as cell wall-mimicking matrix to understand the impact of this matrix and its (micro) structure on the enzyme’s diffusion within it. The enzyme mobility was followed by synchrotron microscopy thanks to its auto-fluorescence after deep-UV excitation. Time-lapse imaging and quantification of intensity signal by image analysis revealed that the diffusion of the enzyme was impacted by at least two criteria: (i) only the active enzyme was able to diffuse, showing that the mobility was related to the catalytic ability, and (ii) the diffusion was improved by the presence of cellulose in the gel.Estelle BonninCamille AlvaradoMarie-Jeanne CrépeauBrigitte BouchetCatherine GarnierFrédéric JammeMarie-Françoise DevauxNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Estelle Bonnin Camille Alvarado Marie-Jeanne Crépeau Brigitte Bouchet Catherine Garnier Frédéric Jamme Marie-Françoise Devaux Mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity
description	Abstract The pectin methylesterase action is usually studied in a homogeneous aqueous medium in the presence of a large excess of soluble substrate and water. However in the cell wall, the water content is much lower, the substrate is cross-linked with itself or with other polymers, and the enzyme has to diffuse through the solid matrix before catalysing the linkage breakdown. As plant primary cell walls can be considered as cellulose-reinforced hydrogels, this study investigated the diffusion of a fungal pectin methylesterase in pectin/cellulose gels used as cell wall-mimicking matrix to understand the impact of this matrix and its (micro) structure on the enzyme’s diffusion within it. The enzyme mobility was followed by synchrotron microscopy thanks to its auto-fluorescence after deep-UV excitation. Time-lapse imaging and quantification of intensity signal by image analysis revealed that the diffusion of the enzyme was impacted by at least two criteria: (i) only the active enzyme was able to diffuse, showing that the mobility was related to the catalytic ability, and (ii) the diffusion was improved by the presence of cellulose in the gel.
format	article
author	Estelle Bonnin Camille Alvarado Marie-Jeanne Crépeau Brigitte Bouchet Catherine Garnier Frédéric Jamme Marie-Françoise Devaux
author_facet	Estelle Bonnin Camille Alvarado Marie-Jeanne Crépeau Brigitte Bouchet Catherine Garnier Frédéric Jamme Marie-Françoise Devaux
author_sort	Estelle Bonnin
title	Mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity
title_short	Mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity
title_full	Mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity
title_fullStr	Mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity
title_full_unstemmed	Mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity
title_sort	mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/f3235ae88af14d1caea39ad1936801df
work_keys_str_mv	AT estellebonnin mobilityofpectinmethylesteraseinpectincellulosegelsisenhancedbythepresenceofcelluloseandbyitscatalyticcapacity AT camillealvarado mobilityofpectinmethylesteraseinpectincellulosegelsisenhancedbythepresenceofcelluloseandbyitscatalyticcapacity AT mariejeannecrepeau mobilityofpectinmethylesteraseinpectincellulosegelsisenhancedbythepresenceofcelluloseandbyitscatalyticcapacity AT brigittebouchet mobilityofpectinmethylesteraseinpectincellulosegelsisenhancedbythepresenceofcelluloseandbyitscatalyticcapacity AT catherinegarnier mobilityofpectinmethylesteraseinpectincellulosegelsisenhancedbythepresenceofcelluloseandbyitscatalyticcapacity AT fredericjamme mobilityofpectinmethylesteraseinpectincellulosegelsisenhancedbythepresenceofcelluloseandbyitscatalyticcapacity AT mariefrancoisedevaux mobilityofpectinmethylesteraseinpectincellulosegelsisenhancedbythepresenceofcelluloseandbyitscatalyticcapacity
_version_	1718388358136201216

Mobility of pectin methylesterase in pectin/cellulose gels is enhanced by the presence of cellulose and by its catalytic capacity

Ejemplares similares