A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases

Püllmann et al developed a modular Golden Gate-based secretion system, which enabled production and one-step purification of active fungal unspecific peroxygenases (UPOs) in yeast. Their system was applied to an enantioselective conversion on a preparative scale and may be used in the future for oth...

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Bibliographic Details
Main Authors: Pascal Püllmann, Anja Knorrscheidt, Judith Münch, Paul R. Palme, Wolfgang Hoehenwarter, Sylvestre Marillonnet, Miguel Alcalde, Bernhard Westermann, Martin J. Weissenborn
Format: article
Language:EN
Published: Nature Portfolio 2021
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Online Access:https://doaj.org/article/f3c63e6d684b4ea8b0fbe616270d80cf
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Summary:Püllmann et al developed a modular Golden Gate-based secretion system, which enabled production and one-step purification of active fungal unspecific peroxygenases (UPOs) in yeast. Their system was applied to an enantioselective conversion on a preparative scale and may be used in the future for other genes of interest that are suitable for production in yeast.