Nerve Growth Factor (NGF) modulates in vitro induced myofibroblasts by highlighting a differential protein signature

Abstract We previously described the profibrogenic effect of NGF on conjunctival Fibroblasts (FBs) and its ability to trigger apoptosis in TGFβ1-induced myofibroblasts (myoFBs). Herein, cell apoptosis/signalling, cytokines’ signature in conditioned media and inflammatory as well as angiogenic pathwa...

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Autores principales:	Graziana Esposito, Bijorn Omar Balzamino, Egidio Stigliano, Filippo Biamonte, Andrea Urbani, Alessandra Micera
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3f8d4a5e47740d4ab769020c370618d
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spelling	oai:doaj.org-article:f3f8d4a5e47740d4ab769020c370618d2021-12-02T15:23:39ZNerve Growth Factor (NGF) modulates in vitro induced myofibroblasts by highlighting a differential protein signature10.1038/s41598-021-81040-x2045-2322https://doaj.org/article/f3f8d4a5e47740d4ab769020c370618d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81040-xhttps://doaj.org/toc/2045-2322Abstract We previously described the profibrogenic effect of NGF on conjunctival Fibroblasts (FBs) and its ability to trigger apoptosis in TGFβ1-induced myofibroblasts (myoFBs). Herein, cell apoptosis/signalling, cytokines’ signature in conditioned media and inflammatory as well as angiogenic pathway were investigated. Experimental myoFBs were exposed to NGF (0.1–100 ng/mL), at defined time-point for confocal and biomolecular analysis. Cells were analysed for apoptotic and cell signalling activation in cell extracts and for some inflammatory and proinflammatory/angiogenic factors’ activations. NGF triggered cJun overexpression and phospho-p65-NFkB nuclear translocation. A decreased Bcl2:Bax ratio and a significant expression of smad7 were confirmed in early AnnexinV-positive myoFBs. A specific protein signature characterised the conditioned media: a dose dependent decrease occurred for IL8, IL6 while a selective increase was observed for VEGF and cyr61 (protein/mRNA). TIMP1 levels were unaffected. Herein, NGF modulation of smad7, the specific IL8 and IL6 as well as VEGF and cyr61 modulation deserve more attention as opening to alternative approaches to counteract fibrosis.Graziana EspositoBijorn Omar BalzaminoEgidio StiglianoFilippo BiamonteAndrea UrbaniAlessandra MiceraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Graziana Esposito Bijorn Omar Balzamino Egidio Stigliano Filippo Biamonte Andrea Urbani Alessandra Micera Nerve Growth Factor (NGF) modulates in vitro induced myofibroblasts by highlighting a differential protein signature
description	Abstract We previously described the profibrogenic effect of NGF on conjunctival Fibroblasts (FBs) and its ability to trigger apoptosis in TGFβ1-induced myofibroblasts (myoFBs). Herein, cell apoptosis/signalling, cytokines’ signature in conditioned media and inflammatory as well as angiogenic pathway were investigated. Experimental myoFBs were exposed to NGF (0.1–100 ng/mL), at defined time-point for confocal and biomolecular analysis. Cells were analysed for apoptotic and cell signalling activation in cell extracts and for some inflammatory and proinflammatory/angiogenic factors’ activations. NGF triggered cJun overexpression and phospho-p65-NFkB nuclear translocation. A decreased Bcl2:Bax ratio and a significant expression of smad7 were confirmed in early AnnexinV-positive myoFBs. A specific protein signature characterised the conditioned media: a dose dependent decrease occurred for IL8, IL6 while a selective increase was observed for VEGF and cyr61 (protein/mRNA). TIMP1 levels were unaffected. Herein, NGF modulation of smad7, the specific IL8 and IL6 as well as VEGF and cyr61 modulation deserve more attention as opening to alternative approaches to counteract fibrosis.
format	article
author	Graziana Esposito Bijorn Omar Balzamino Egidio Stigliano Filippo Biamonte Andrea Urbani Alessandra Micera
author_facet	Graziana Esposito Bijorn Omar Balzamino Egidio Stigliano Filippo Biamonte Andrea Urbani Alessandra Micera
author_sort	Graziana Esposito
title	Nerve Growth Factor (NGF) modulates in vitro induced myofibroblasts by highlighting a differential protein signature
title_short	Nerve Growth Factor (NGF) modulates in vitro induced myofibroblasts by highlighting a differential protein signature
title_full	Nerve Growth Factor (NGF) modulates in vitro induced myofibroblasts by highlighting a differential protein signature
title_fullStr	Nerve Growth Factor (NGF) modulates in vitro induced myofibroblasts by highlighting a differential protein signature
title_full_unstemmed	Nerve Growth Factor (NGF) modulates in vitro induced myofibroblasts by highlighting a differential protein signature
title_sort	nerve growth factor (ngf) modulates in vitro induced myofibroblasts by highlighting a differential protein signature
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f3f8d4a5e47740d4ab769020c370618d
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Nerve Growth Factor (NGF) modulates in vitro induced myofibroblasts by highlighting a differential protein signature

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