Development of a simple, rapid multiplex PCR tool kit by using the 16S rRNA gene for the identification of faecal and non-faecal coliforms in drinking water

A multiplex method for the detection of faecal and non-faecal coliforms in drinking water was developed using three primers from the V2, V3 and V9 variable regions of the 16S rRNA gene. 194F, 474F and 1436R are the three primers designed for specific amplification of the V2, V3 and V9 hyper variable...

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Autores principales: A. Shiva Shanker, N. Rajesh, Pavan Kumar Pindi
Formato: article
Lenguaje:EN
Publicado: IWA Publishing 2021
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Acceso en línea:https://doaj.org/article/f480adbc753b4bdabbcb9d129decc1ff
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