Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant

Manipulating DNA repair pathways can be used to improve the outcomes of CRISPR-based genome editing. Here the authors derive an enhanced RAD18 variant that suppresses 53BP1 recruitment to DNA double-strand breaks to enhance homology-mediated repair.

Guardado en:

Detalles Bibliográficos
Autores principales:	Tarun S. Nambiar, Pierre Billon, Giacomo Diedenhofen, Samuel B. Hayward, Angelo Taglialatela, Kunheng Cai, Jen-Wei Huang, Giuseppe Leuzzi, Raquel Cuella-Martin, Andrew Palacios, Anuj Gupta, Dieter Egli, Alberto Ciccia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/f4d885e322c6447198d8c7f4fa611f33
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f4d885e322c6447198d8c7f4fa611f33
record_format	dspace
spelling	oai:doaj.org-article:f4d885e322c6447198d8c7f4fa611f332021-12-02T15:35:57ZStimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant10.1038/s41467-019-11105-z2041-1723https://doaj.org/article/f4d885e322c6447198d8c7f4fa611f332019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11105-zhttps://doaj.org/toc/2041-1723Manipulating DNA repair pathways can be used to improve the outcomes of CRISPR-based genome editing. Here the authors derive an enhanced RAD18 variant that suppresses 53BP1 recruitment to DNA double-strand breaks to enhance homology-mediated repair.Tarun S. NambiarPierre BillonGiacomo DiedenhofenSamuel B. HaywardAngelo TaglialatelaKunheng CaiJen-Wei HuangGiuseppe LeuzziRaquel Cuella-MartinAndrew PalaciosAnuj GuptaDieter EgliAlberto CicciaNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Tarun S. Nambiar Pierre Billon Giacomo Diedenhofen Samuel B. Hayward Angelo Taglialatela Kunheng Cai Jen-Wei Huang Giuseppe Leuzzi Raquel Cuella-Martin Andrew Palacios Anuj Gupta Dieter Egli Alberto Ciccia Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant
description	Manipulating DNA repair pathways can be used to improve the outcomes of CRISPR-based genome editing. Here the authors derive an enhanced RAD18 variant that suppresses 53BP1 recruitment to DNA double-strand breaks to enhance homology-mediated repair.
format	article
author	Tarun S. Nambiar Pierre Billon Giacomo Diedenhofen Samuel B. Hayward Angelo Taglialatela Kunheng Cai Jen-Wei Huang Giuseppe Leuzzi Raquel Cuella-Martin Andrew Palacios Anuj Gupta Dieter Egli Alberto Ciccia
author_facet	Tarun S. Nambiar Pierre Billon Giacomo Diedenhofen Samuel B. Hayward Angelo Taglialatela Kunheng Cai Jen-Wei Huang Giuseppe Leuzzi Raquel Cuella-Martin Andrew Palacios Anuj Gupta Dieter Egli Alberto Ciccia
author_sort	Tarun S. Nambiar
title	Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant
title_short	Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant
title_full	Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant
title_fullStr	Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant
title_full_unstemmed	Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant
title_sort	stimulation of crispr-mediated homology-directed repair by an engineered rad18 variant
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/f4d885e322c6447198d8c7f4fa611f33
work_keys_str_mv	AT tarunsnambiar stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT pierrebillon stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT giacomodiedenhofen stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT samuelbhayward stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT angelotaglialatela stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT kunhengcai stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT jenweihuang stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT giuseppeleuzzi stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT raquelcuellamartin stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT andrewpalacios stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT anujgupta stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT dieteregli stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant AT albertociccia stimulationofcrisprmediatedhomologydirectedrepairbyanengineeredrad18variant
_version_	1718386450320326656

Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant

Ejemplares similares