Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching

Abstract High-throughput single-stranded DNA sequencing (ssDNA-seq) of cell-free DNA from plasma and other bodily fluids is a powerful method for non-invasive prenatal testing, and diagnosis of cancers and other diseases. Here, we developed a facile ssDNA-seq method, which exploits a novel template-...

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Autores principales: Douglas C. Wu, Alan M. Lambowitz
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/f6d262c943124d31bc0c968b144146f6
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