PROCEDURE OF QUANTITATIVE DETERMINATION OF THE TOTAL FLAVONOID CONTENT IN THE UNIFLOROUS VIOLET

The object of the study was the aerial part of flowered violet (Viola uniflora L.), harvested at flowering time in the Irkutsk region. The herb contains flavonoids, phenolic acids, coumarins, tannins, polysaccharides, fatty acids and amino acids, as well as macro- and microelements. During the devel...

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Autores principales:	A. M. Martynov, T. D. Dargaeva
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2017
Materias:	uniflorous violet flavonoids spectrophotometry quantitative determination Science Q
Acceso en línea:	https://doaj.org/article/f7010c57c4cc409d8fce0a7aaad869f6
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spelling	oai:doaj.org-article:f7010c57c4cc409d8fce0a7aaad869f62021-11-23T06:14:37ZPROCEDURE OF QUANTITATIVE DETERMINATION OF THE TOTAL FLAVONOID CONTENT IN THE UNIFLOROUS VIOLET2541-94202587-959610.12737/article_5955e6b58f6ae1.34521097https://doaj.org/article/f7010c57c4cc409d8fce0a7aaad869f62017-02-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/368https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596The object of the study was the aerial part of flowered violet (Viola uniflora L.), harvested at flowering time in the Irkutsk region. The herb contains flavonoids, phenolic acids, coumarins, tannins, polysaccharides, fatty acids and amino acids, as well as macro- and microelements. During the development of methods of quantitative determination of flavonoid compounds we experimentally established optimum conditions for their extraction from vegetable raw materials: the degree of grinding - 2 mm, extractant - 70% ethyl alcohol, the ratio of raw material - extractant 1:50 total extraction duration -1 hour (twice), time complexation reaction with a 5% ethanolic solution of aluminum chloride is 30 minutes. Stability of the complex was maintained for 1.5 hours. Quantitation of flavonoid compounds in the raw material were determined by differential spectrophotometry based on rutin. The optical density of the solution was measured on a recording spectrophotometer "Lambda 35 UV/VIS" Perkin Elmer instruments (USA) at a wavelength of 409 ± 2 nm in a cell with a working thickness of 10 mm. The content of flavonoids in terms of rutin in different samples ranged from 1.66 to 2.03 %. The developed method of quantitative spectrophotometric determination of the amount of flavonoids in Viola uniflora grass validated in terms of specificity, precision (convergence), linearity, accuracy and set its range of applications. According to the results of validation techniques quantification procedure of flavonoids Viola uniflora grass it has been found that the technique is reproducible and can be used in quantitative analysis.A. M. MartynovT. D. DargaevaScientific Сentre for Family Health and Human Reproduction Problemsarticleuniflorous violetflavonoidsspectrophotometryquantitative determinationScienceQRUActa Biomedica Scientifica, Vol 2, Iss 1, Pp 79-83 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	uniflorous violet flavonoids spectrophotometry quantitative determination Science Q
spellingShingle	uniflorous violet flavonoids spectrophotometry quantitative determination Science Q A. M. Martynov T. D. Dargaeva PROCEDURE OF QUANTITATIVE DETERMINATION OF THE TOTAL FLAVONOID CONTENT IN THE UNIFLOROUS VIOLET
description	The object of the study was the aerial part of flowered violet (Viola uniflora L.), harvested at flowering time in the Irkutsk region. The herb contains flavonoids, phenolic acids, coumarins, tannins, polysaccharides, fatty acids and amino acids, as well as macro- and microelements. During the development of methods of quantitative determination of flavonoid compounds we experimentally established optimum conditions for their extraction from vegetable raw materials: the degree of grinding - 2 mm, extractant - 70% ethyl alcohol, the ratio of raw material - extractant 1:50 total extraction duration -1 hour (twice), time complexation reaction with a 5% ethanolic solution of aluminum chloride is 30 minutes. Stability of the complex was maintained for 1.5 hours. Quantitation of flavonoid compounds in the raw material were determined by differential spectrophotometry based on rutin. The optical density of the solution was measured on a recording spectrophotometer "Lambda 35 UV/VIS" Perkin Elmer instruments (USA) at a wavelength of 409 ± 2 nm in a cell with a working thickness of 10 mm. The content of flavonoids in terms of rutin in different samples ranged from 1.66 to 2.03 %. The developed method of quantitative spectrophotometric determination of the amount of flavonoids in Viola uniflora grass validated in terms of specificity, precision (convergence), linearity, accuracy and set its range of applications. According to the results of validation techniques quantification procedure of flavonoids Viola uniflora grass it has been found that the technique is reproducible and can be used in quantitative analysis.
format	article
author	A. M. Martynov T. D. Dargaeva
author_facet	A. M. Martynov T. D. Dargaeva
author_sort	A. M. Martynov
title	PROCEDURE OF QUANTITATIVE DETERMINATION OF THE TOTAL FLAVONOID CONTENT IN THE UNIFLOROUS VIOLET
title_short	PROCEDURE OF QUANTITATIVE DETERMINATION OF THE TOTAL FLAVONOID CONTENT IN THE UNIFLOROUS VIOLET
title_full	PROCEDURE OF QUANTITATIVE DETERMINATION OF THE TOTAL FLAVONOID CONTENT IN THE UNIFLOROUS VIOLET
title_fullStr	PROCEDURE OF QUANTITATIVE DETERMINATION OF THE TOTAL FLAVONOID CONTENT IN THE UNIFLOROUS VIOLET
title_full_unstemmed	PROCEDURE OF QUANTITATIVE DETERMINATION OF THE TOTAL FLAVONOID CONTENT IN THE UNIFLOROUS VIOLET
title_sort	procedure of quantitative determination of the total flavonoid content in the uniflorous violet
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2017
url	https://doaj.org/article/f7010c57c4cc409d8fce0a7aaad869f6
work_keys_str_mv	AT ammartynov procedureofquantitativedeterminationofthetotalflavonoidcontentintheuniflorousviolet AT tddargaeva procedureofquantitativedeterminationofthetotalflavonoidcontentintheuniflorousviolet
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PROCEDURE OF QUANTITATIVE DETERMINATION OF THE TOTAL FLAVONOID CONTENT IN THE UNIFLOROUS VIOLET

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