Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds

Introduction. It is known that the enzymes catalase (CT) and superoxide dismutase (SOD) are actively involved in protecting cells of microbial pathogens against the biochemical factors produced by macrophages in vivo, in order to stop infection. Therefore, this study was aimed to quantify the parame...

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Autores principales:	Carolina LOZAN-TIRSU, Elena ZARICIUC
Formato:	article
Lenguaje:	EN FR RO RU
Publicado:	Asociatia de Biosiguranta si Biosecuritate 2021
Materias:	antimicrobial activity antioxidant-enzyme activity chemical compounds ct sod Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f82964c2d0a949aba87c3a8589fadeee
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spelling	oai:doaj.org-article:f82964c2d0a949aba87c3a8589fadeee2021-12-02T17:40:20ZBiochemical composition changes of gram-negative microorganisms under the action of new chemical compounds10.38045/ohrm.2021.3.092887-34582587-3466https://doaj.org/article/f82964c2d0a949aba87c3a8589fadeee2021-06-01T00:00:00Zhttps://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/140/98https://doaj.org/toc/2887-3458https://doaj.org/toc/2587-3466Introduction. It is known that the enzymes catalase (CT) and superoxide dismutase (SOD) are actively involved in protecting cells of microbial pathogens against the biochemical factors produced by macrophages in vivo, in order to stop infection. Therefore, this study was aimed to quantify the parameters of the antioxidant-enzyme activities within the reference cultures under the action of selected new chemical compounds. Material and methods. Cu (II) coordination compounds, Co (II) and Zn (II) were included as “in vitro” study material, as well as aromatic propenones synthesized at the Department of Inorganic Chemistry, at State University of Moldova. The antimicrobial effects were tested on three gram-negative reference strains. The enzymatic activity of SOD and the CT activity was determined to assess the pathogen-induced changes in the antioxidant status under the action of newly tested compounds. Results. The new chemical compounds showed a significant decrease in the antioxidant enzymatic activities of SOD and CT, induced in all reference strains used, which indicates an intense oxidative stress generated by the tested compounds. Conclusions. The specific activity of new chemical compounds on primary antioxidant enzymes, which represent pathogenicity factors of infectious agents, allows us to assume the benefits of in vivo effect of new native substances, as well as to recommend the selected compounds for further biomedical tests.Carolina LOZAN-TIRSUElena ZARICIUCAsociatia de Biosiguranta si Biosecuritatearticleantimicrobial activityantioxidant-enzyme activitychemical compoundsctsodMedicineRScienceQENFRRORUOne Health & Risk Management, Vol 2, Iss 3, Pp 55-60 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR RO RU
topic	antimicrobial activity antioxidant-enzyme activity chemical compounds ct sod Medicine R Science Q
spellingShingle	antimicrobial activity antioxidant-enzyme activity chemical compounds ct sod Medicine R Science Q Carolina LOZAN-TIRSU Elena ZARICIUC Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
description	Introduction. It is known that the enzymes catalase (CT) and superoxide dismutase (SOD) are actively involved in protecting cells of microbial pathogens against the biochemical factors produced by macrophages in vivo, in order to stop infection. Therefore, this study was aimed to quantify the parameters of the antioxidant-enzyme activities within the reference cultures under the action of selected new chemical compounds. Material and methods. Cu (II) coordination compounds, Co (II) and Zn (II) were included as “in vitro” study material, as well as aromatic propenones synthesized at the Department of Inorganic Chemistry, at State University of Moldova. The antimicrobial effects were tested on three gram-negative reference strains. The enzymatic activity of SOD and the CT activity was determined to assess the pathogen-induced changes in the antioxidant status under the action of newly tested compounds. Results. The new chemical compounds showed a significant decrease in the antioxidant enzymatic activities of SOD and CT, induced in all reference strains used, which indicates an intense oxidative stress generated by the tested compounds. Conclusions. The specific activity of new chemical compounds on primary antioxidant enzymes, which represent pathogenicity factors of infectious agents, allows us to assume the benefits of in vivo effect of new native substances, as well as to recommend the selected compounds for further biomedical tests.
format	article
author	Carolina LOZAN-TIRSU Elena ZARICIUC
author_facet	Carolina LOZAN-TIRSU Elena ZARICIUC
author_sort	Carolina LOZAN-TIRSU
title	Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
title_short	Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
title_full	Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
title_fullStr	Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
title_full_unstemmed	Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
title_sort	biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
publisher	Asociatia de Biosiguranta si Biosecuritate
publishDate	2021
url	https://doaj.org/article/f82964c2d0a949aba87c3a8589fadeee
work_keys_str_mv	AT carolinalozantirsu biochemicalcompositionchangesofgramnegativemicroorganismsundertheactionofnewchemicalcompounds AT elenazariciuc biochemicalcompositionchangesofgramnegativemicroorganismsundertheactionofnewchemicalcompounds
_version_	1718379801041960960

Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds

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