Isolation, identification and utilization of lactic acid bacteria from silage in a warm and humid climate area

Abstract The study aimed to isolate and identify lactic acid bacteria (LAB) from silages and their application to improve the fermentation quality of alfalfa. Forty-nine LAB strains were isolated from silages, and two strains were screened for growth and acid production rates. Then two strains were...

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Autores principales:	Chao Peng, Wentao Sun, Xiang Dong, Lili Zhao, Jun Hao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8910af0d1ef480e838fa330aecd1b60
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spelling	oai:doaj.org-article:f8910af0d1ef480e838fa330aecd1b602021-12-02T16:04:26ZIsolation, identification and utilization of lactic acid bacteria from silage in a warm and humid climate area10.1038/s41598-021-92034-02045-2322https://doaj.org/article/f8910af0d1ef480e838fa330aecd1b602021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92034-0https://doaj.org/toc/2045-2322Abstract The study aimed to isolate and identify lactic acid bacteria (LAB) from silages and their application to improve the fermentation quality of alfalfa. Forty-nine LAB strains were isolated from silages, and two strains were screened for growth and acid production rates. Then two strains were selected for Physiological and morphological tests and 16S rRNA sequencing. They were Gram-positive and Catalase-negative and were able to grow at pH 3.5 and at 45 °C, were unable to grow different NaCl concentrations as 3.0% and 6.5%. Strain BDy3-10 was identified as Lactobacillus rhamnosus, while TSy1-3 was identified as L. buchneri. The selected strains were evaluated on fermentation of alfalfa silage. The highest crude protein content occurred in the BDy3-10 treatment group. The contents of neutral detergent fiber and acid detergent fiber in the TSy1-3 treatment were significantly lower than other treatment (P < 0.05). Compared to the control treatment, inoculation treatments deceased pH during ensiling (P < 0.001) and provided the most increased lactic acid content after ensiling for 10 days (P < 0.001). The acetic acid contents of all the inoculation groups were significantly increased (P < 0.001) during ensiling, and were lower than that of control group (P < 0.001). So, the TSy1-3 treatment most effectively improved the fermentation quality of alfalfa silage in warm and humid climate area.Chao PengWentao SunXiang DongLili ZhaoJun HaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chao Peng Wentao Sun Xiang Dong Lili Zhao Jun Hao Isolation, identification and utilization of lactic acid bacteria from silage in a warm and humid climate area
description	Abstract The study aimed to isolate and identify lactic acid bacteria (LAB) from silages and their application to improve the fermentation quality of alfalfa. Forty-nine LAB strains were isolated from silages, and two strains were screened for growth and acid production rates. Then two strains were selected for Physiological and morphological tests and 16S rRNA sequencing. They were Gram-positive and Catalase-negative and were able to grow at pH 3.5 and at 45 °C, were unable to grow different NaCl concentrations as 3.0% and 6.5%. Strain BDy3-10 was identified as Lactobacillus rhamnosus, while TSy1-3 was identified as L. buchneri. The selected strains were evaluated on fermentation of alfalfa silage. The highest crude protein content occurred in the BDy3-10 treatment group. The contents of neutral detergent fiber and acid detergent fiber in the TSy1-3 treatment were significantly lower than other treatment (P < 0.05). Compared to the control treatment, inoculation treatments deceased pH during ensiling (P < 0.001) and provided the most increased lactic acid content after ensiling for 10 days (P < 0.001). The acetic acid contents of all the inoculation groups were significantly increased (P < 0.001) during ensiling, and were lower than that of control group (P < 0.001). So, the TSy1-3 treatment most effectively improved the fermentation quality of alfalfa silage in warm and humid climate area.
format	article
author	Chao Peng Wentao Sun Xiang Dong Lili Zhao Jun Hao
author_facet	Chao Peng Wentao Sun Xiang Dong Lili Zhao Jun Hao
author_sort	Chao Peng
title	Isolation, identification and utilization of lactic acid bacteria from silage in a warm and humid climate area
title_short	Isolation, identification and utilization of lactic acid bacteria from silage in a warm and humid climate area
title_full	Isolation, identification and utilization of lactic acid bacteria from silage in a warm and humid climate area
title_fullStr	Isolation, identification and utilization of lactic acid bacteria from silage in a warm and humid climate area
title_full_unstemmed	Isolation, identification and utilization of lactic acid bacteria from silage in a warm and humid climate area
title_sort	isolation, identification and utilization of lactic acid bacteria from silage in a warm and humid climate area
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f8910af0d1ef480e838fa330aecd1b60
work_keys_str_mv	AT chaopeng isolationidentificationandutilizationoflacticacidbacteriafromsilageinawarmandhumidclimatearea AT wentaosun isolationidentificationandutilizationoflacticacidbacteriafromsilageinawarmandhumidclimatearea AT xiangdong isolationidentificationandutilizationoflacticacidbacteriafromsilageinawarmandhumidclimatearea AT lilizhao isolationidentificationandutilizationoflacticacidbacteriafromsilageinawarmandhumidclimatearea AT junhao isolationidentificationandutilizationoflacticacidbacteriafromsilageinawarmandhumidclimatearea
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Isolation, identification and utilization of lactic acid bacteria from silage in a warm and humid climate area

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