Identification of a 57S translation complex containing closed-loop factors and the 60S ribosome subunit

Abstract In eukaryotic translation the 60S ribosome subunit has not been proposed to interact with mRNA or closed-loop factors eIF4E, eIF4G, and PAB1. Using analytical ultracentrifugation with fluorescent detection system, we have identified a 57S translation complex that contains the 60S ribosome,...

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Autores principales:	Clyde L. Denis, Thomas M. Laue, Xin Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f913d2dae08f435f983fd4a9a50939cb
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spelling	oai:doaj.org-article:f913d2dae08f435f983fd4a9a50939cb2021-12-02T15:08:40ZIdentification of a 57S translation complex containing closed-loop factors and the 60S ribosome subunit10.1038/s41598-018-29832-62045-2322https://doaj.org/article/f913d2dae08f435f983fd4a9a50939cb2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29832-6https://doaj.org/toc/2045-2322Abstract In eukaryotic translation the 60S ribosome subunit has not been proposed to interact with mRNA or closed-loop factors eIF4E, eIF4G, and PAB1. Using analytical ultracentrifugation with fluorescent detection system, we have identified a 57S translation complex that contains the 60S ribosome, mRNA, and the closed-loop factors. Previously published data by others also indicate the presence of a 50S-60S translation complex containing these same components. We have found that the abundance of this complex increased upon translational cessation, implying formation after ribosomal dissociation. Stoichiometric analyses of the abundances of the closed-loop components in the 57S complex indicate this complex is most similar to polysomal and monosomal translation complexes at the end of translation rather than at the beginning or middle of translation. In contrast, a 39S complex containing the 40S ribosome bound to mRNA and closed-loop factors was also identified with stoichiometries most similar to polysomal complexes engaged in translation, suggesting that the 39S complex is the previously studied 48S translation initiation complex. These results indicate that the 60S ribosome can associate with the closed-loop mRNA structure and plays a previously undetected role in the translation process.Clyde L. DenisThomas M. LaueXin WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Clyde L. Denis Thomas M. Laue Xin Wang Identification of a 57S translation complex containing closed-loop factors and the 60S ribosome subunit
description	Abstract In eukaryotic translation the 60S ribosome subunit has not been proposed to interact with mRNA or closed-loop factors eIF4E, eIF4G, and PAB1. Using analytical ultracentrifugation with fluorescent detection system, we have identified a 57S translation complex that contains the 60S ribosome, mRNA, and the closed-loop factors. Previously published data by others also indicate the presence of a 50S-60S translation complex containing these same components. We have found that the abundance of this complex increased upon translational cessation, implying formation after ribosomal dissociation. Stoichiometric analyses of the abundances of the closed-loop components in the 57S complex indicate this complex is most similar to polysomal and monosomal translation complexes at the end of translation rather than at the beginning or middle of translation. In contrast, a 39S complex containing the 40S ribosome bound to mRNA and closed-loop factors was also identified with stoichiometries most similar to polysomal complexes engaged in translation, suggesting that the 39S complex is the previously studied 48S translation initiation complex. These results indicate that the 60S ribosome can associate with the closed-loop mRNA structure and plays a previously undetected role in the translation process.
format	article
author	Clyde L. Denis Thomas M. Laue Xin Wang
author_facet	Clyde L. Denis Thomas M. Laue Xin Wang
author_sort	Clyde L. Denis
title	Identification of a 57S translation complex containing closed-loop factors and the 60S ribosome subunit
title_short	Identification of a 57S translation complex containing closed-loop factors and the 60S ribosome subunit
title_full	Identification of a 57S translation complex containing closed-loop factors and the 60S ribosome subunit
title_fullStr	Identification of a 57S translation complex containing closed-loop factors and the 60S ribosome subunit
title_full_unstemmed	Identification of a 57S translation complex containing closed-loop factors and the 60S ribosome subunit
title_sort	identification of a 57s translation complex containing closed-loop factors and the 60s ribosome subunit
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f913d2dae08f435f983fd4a9a50939cb
work_keys_str_mv	AT clydeldenis identificationofa57stranslationcomplexcontainingclosedloopfactorsandthe60sribosomesubunit AT thomasmlaue identificationofa57stranslationcomplexcontainingclosedloopfactorsandthe60sribosomesubunit AT xinwang identificationofa57stranslationcomplexcontainingclosedloopfactorsandthe60sribosomesubunit
_version_	1718388100573429760

Identification of a 57S translation complex containing closed-loop factors and the 60S ribosome subunit

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