Gut Microbiome Profiling Uncovers a Lower Abundance of <i>Butyricicoccus</i> in Advanced Stages of Chronic Kidney Disease

Chronic kidney disease (CKD) is characterized by the accumulation of uremic toxins which exert deleterious effects on various organ systems. Several of these uremic toxins originate from the bacterial metabolization of aromatic amino acids in the colon. This study assessed whether the gut microbial...

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Autores principales:	Tessa Gryp, Karoline Faust, Wim Van Biesen, Geert R. B. Huys, Francis Verbeke, Marijn Speeckaert, Jeroen Raes, Mario Vaneechoutte, Marie Joossens, Griet Glorieux
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chronic kidney disease uremic toxins gut microbial composition <i>p</i>-cresyl sulfate <i>p</i>-cresyl glucuronide Medicine R
Acceso en línea:	https://doaj.org/article/fa12ff1421044446a9f285b55db003d4
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spelling	oai:doaj.org-article:fa12ff1421044446a9f285b55db003d42021-11-25T18:07:20ZGut Microbiome Profiling Uncovers a Lower Abundance of <i>Butyricicoccus</i> in Advanced Stages of Chronic Kidney Disease10.3390/jpm111111182075-4426https://doaj.org/article/fa12ff1421044446a9f285b55db003d42021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4426/11/11/1118https://doaj.org/toc/2075-4426Chronic kidney disease (CKD) is characterized by the accumulation of uremic toxins which exert deleterious effects on various organ systems. Several of these uremic toxins originate from the bacterial metabolization of aromatic amino acids in the colon. This study assessed whether the gut microbial composition varies among patients in different stages of CKD. Uremic metabolites were quantified by UPLC/fluorescence detection and microbial profiling by 16S rRNA amplicon sequencing. Gut microbial profiles of CKD patients were compared among stages 1–2, stage 3 and stages 4–5. Although a substantial inter-individual difference in abundance of the top 15 genera was observed, no significant difference was observed between groups. Bristol stool scale (BSS) correlated negatively with <i>p</i>-cresyl sulfate and hippuric acid levels, irrespective of the intake of laxatives. <i>Butyricicoccus</i>, a genus with butyrate-generating properties, was decreased in abundance in advanced stages of CKD compared to the earlier stages (<i>p</i> = 0.043). In conclusion, in this cross-sectional study no gradual differences in the gut microbial profile over the different stages of CKD were observed. However, the decrease in the abundance of <i>Butyricicoccus</i> genus with loss of kidney function stresses the need for more in-depth functional exploration of the gut microbiome in CKD patients not on dialysis.Tessa GrypKaroline FaustWim Van BiesenGeert R. B. HuysFrancis VerbekeMarijn SpeeckaertJeroen RaesMario VaneechoutteMarie JoossensGriet GlorieuxMDPI AGarticlechronic kidney diseaseuremic toxinsgut microbial composition<i>p</i>-cresyl sulfate<i>p</i>-cresyl glucuronideMedicineRENJournal of Personalized Medicine, Vol 11, Iss 1118, p 1118 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chronic kidney disease uremic toxins gut microbial composition <i>p</i>-cresyl sulfate <i>p</i>-cresyl glucuronide Medicine R
spellingShingle	chronic kidney disease uremic toxins gut microbial composition <i>p</i>-cresyl sulfate <i>p</i>-cresyl glucuronide Medicine R Tessa Gryp Karoline Faust Wim Van Biesen Geert R. B. Huys Francis Verbeke Marijn Speeckaert Jeroen Raes Mario Vaneechoutte Marie Joossens Griet Glorieux Gut Microbiome Profiling Uncovers a Lower Abundance of <i>Butyricicoccus</i> in Advanced Stages of Chronic Kidney Disease
description	Chronic kidney disease (CKD) is characterized by the accumulation of uremic toxins which exert deleterious effects on various organ systems. Several of these uremic toxins originate from the bacterial metabolization of aromatic amino acids in the colon. This study assessed whether the gut microbial composition varies among patients in different stages of CKD. Uremic metabolites were quantified by UPLC/fluorescence detection and microbial profiling by 16S rRNA amplicon sequencing. Gut microbial profiles of CKD patients were compared among stages 1–2, stage 3 and stages 4–5. Although a substantial inter-individual difference in abundance of the top 15 genera was observed, no significant difference was observed between groups. Bristol stool scale (BSS) correlated negatively with <i>p</i>-cresyl sulfate and hippuric acid levels, irrespective of the intake of laxatives. <i>Butyricicoccus</i>, a genus with butyrate-generating properties, was decreased in abundance in advanced stages of CKD compared to the earlier stages (<i>p</i> = 0.043). In conclusion, in this cross-sectional study no gradual differences in the gut microbial profile over the different stages of CKD were observed. However, the decrease in the abundance of <i>Butyricicoccus</i> genus with loss of kidney function stresses the need for more in-depth functional exploration of the gut microbiome in CKD patients not on dialysis.
format	article
author	Tessa Gryp Karoline Faust Wim Van Biesen Geert R. B. Huys Francis Verbeke Marijn Speeckaert Jeroen Raes Mario Vaneechoutte Marie Joossens Griet Glorieux
author_facet	Tessa Gryp Karoline Faust Wim Van Biesen Geert R. B. Huys Francis Verbeke Marijn Speeckaert Jeroen Raes Mario Vaneechoutte Marie Joossens Griet Glorieux
author_sort	Tessa Gryp
title	Gut Microbiome Profiling Uncovers a Lower Abundance of <i>Butyricicoccus</i> in Advanced Stages of Chronic Kidney Disease
title_short	Gut Microbiome Profiling Uncovers a Lower Abundance of <i>Butyricicoccus</i> in Advanced Stages of Chronic Kidney Disease
title_full	Gut Microbiome Profiling Uncovers a Lower Abundance of <i>Butyricicoccus</i> in Advanced Stages of Chronic Kidney Disease
title_fullStr	Gut Microbiome Profiling Uncovers a Lower Abundance of <i>Butyricicoccus</i> in Advanced Stages of Chronic Kidney Disease
title_full_unstemmed	Gut Microbiome Profiling Uncovers a Lower Abundance of <i>Butyricicoccus</i> in Advanced Stages of Chronic Kidney Disease
title_sort	gut microbiome profiling uncovers a lower abundance of <i>butyricicoccus</i> in advanced stages of chronic kidney disease
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fa12ff1421044446a9f285b55db003d4
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Gut Microbiome Profiling Uncovers a Lower Abundance of <i>Butyricicoccus</i> in Advanced Stages of Chronic Kidney Disease

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