Stringent Base Specific and Optimization-Free Multiplex Mediator Probe ddPCR for the Quantification of Point Mutations in Circulating Tumor DNA

Stringent Base Specific and Optimization-Free Multiplex Mediator Probe ddPCR for the Quantification of Point Mutations in Circulating Tumor DNA

There is an increasing demand for optimization-free multiplex assays to rapidly establish comprehensive target panels for cancer monitoring by liquid biopsy. We present the mediator probe (MP) PCR for the quantification of the seven most frequent point mutations and corresponding wild types (<i&g...

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Autores principales: Franziska Schlenker, Elena Kipf, Max Deuter, Inga Höffkes, Michael Lehnert, Roland Zengerle, Felix von Stetten, Florian Scherer, Julius Wehrle, Nikolas von Bubnoff, Peter Juelg, Tobias Hutzenlaub, Nadine Borst
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
mediator probe PCR
digital PCR
multiplex PCR
optimization-free
colorectal carcinoma
standardized universal reporter
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
RC254-282
Acceso en línea:https://doaj.org/article/facbddc1b3114a928e035a7c1228b730
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https://doaj.org/article/facbddc1b3114a928e035a7c1228b730

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