Disinfectant and Antimicrobial Susceptibility Studies of Staphylococcus aureus Strains and ST398-MRSA and ST5-MRSA Strains from Swine Mandibular Lymph Node Tissue, Commercial Pork Sausage Meat and Swine Feces

Staphylococcus aureus (S. aureus) causes gastrointestinal illness worldwide. Disinfectants are used throughout the food chain for pathogenic bacteria control. We investigated S. aureus bioavailability in swine Mandibular lymph node tissue (MLT...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ross C. Beier, Kathleen Andrews, Michael E. Hume, Muhammad Umar Sohail, Roger B. Harvey, Toni L. Poole, Tawni L. Crippen, Robin C. Anderson
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antimicrobial disinfectant pork sausage meat <i>Staphylococcus aureus</i> swine ST398 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fba0ebcfa9a44a4b82f09477741d0a80
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fba0ebcfa9a44a4b82f09477741d0a80
record_format	dspace
spelling	oai:doaj.org-article:fba0ebcfa9a44a4b82f09477741d0a802021-11-25T18:25:42ZDisinfectant and Antimicrobial Susceptibility Studies of <i>Staphylococcus aureus</i> Strains and ST398-MRSA and ST5-MRSA Strains from Swine Mandibular Lymph Node Tissue, Commercial Pork Sausage Meat and Swine Feces10.3390/microorganisms91124012076-2607https://doaj.org/article/fba0ebcfa9a44a4b82f09477741d0a802021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2401https://doaj.org/toc/2076-2607<i>Staphylococcus aureus</i> (<i>S. aureus</i>) causes gastrointestinal illness worldwide. Disinfectants are used throughout the food chain for pathogenic bacteria control. We investigated <i>S. aureus</i> bioavailability in swine Mandibular lymph node tissue (MLT) and pork sausage meat (PSM), established susceptibility values for <i>S. aureus</i> to disinfectants, and determined the multilocus sequence type of MRSA strains. Antimicrobial and disinfectant susceptibility profiles were determined for 164 <i>S. aureus</i> strains isolated from swine feces (<i>n</i> = 63), MLT (<i>n</i> = 49) and PSM (<i>n</i> = 52). No antimicrobial resistance (AMR) was detected to daptomycin, nitrofurantoin, linezolid, and tigecycline, while high AMR prevalence was determined to erythromycin (50.6%), tylosin tartrate (42.7%), penicillin (72%), and tetracycline (68.9%). Methicillin-resistant <i>S. aureus</i> (MRSA) strains, ST398 (<i>n</i> = 6) and ST5 (<i>n</i> = 1), were found in the MLT and PSM, 4 MRSA in MLT and 3 MRSA strains in the PSM. About 17.5% of feces strains and 41.6% of MLT and PSM strains were resistant to chlorhexidine. All strains were susceptible to triclosan and benzalkonium chloride, with no cross-resistance between antimicrobials and disinfectants. Six MRSA strains had elevated susceptibilities to 18 disinfectants. The use of formaldehyde and tris(hydroxylmethyl)nitromethane in DC&R was not effective, which can add chemicals to the environment. Didecyldimethylammonium chloride and benzyldimethylhexadecylammonium chloride were equally effective disinfectants. ST398 and ST5 MRSA strains had elevated susceptibilities to 75% of the disinfectants tested. This study establishes susceptibility values for <i>S. aureus</i> strains from swine feces, mandibular lymph node tissue, and commercial pork sausage against 24 disinfectants. Since it was demonstrated that <i>S. aureus</i> and MRSA strains can be found deep within swine lymph node tissue, it may be beneficial for the consumer if raw swine lymph node tissue is not used in uncooked food products and pork sausage.Ross C. BeierKathleen AndrewsMichael E. HumeMuhammad Umar SohailRoger B. HarveyToni L. PooleTawni L. CrippenRobin C. AndersonMDPI AGarticleantimicrobialdisinfectantpork sausage meat<i>Staphylococcus aureus</i>swineST398Biology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2401, p 2401 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial disinfectant pork sausage meat <i>Staphylococcus aureus</i> swine ST398 Biology (General) QH301-705.5
spellingShingle	antimicrobial disinfectant pork sausage meat <i>Staphylococcus aureus</i> swine ST398 Biology (General) QH301-705.5 Ross C. Beier Kathleen Andrews Michael E. Hume Muhammad Umar Sohail Roger B. Harvey Toni L. Poole Tawni L. Crippen Robin C. Anderson Disinfectant and Antimicrobial Susceptibility Studies of <i>Staphylococcus aureus</i> Strains and ST398-MRSA and ST5-MRSA Strains from Swine Mandibular Lymph Node Tissue, Commercial Pork Sausage Meat and Swine Feces
description	<i>Staphylococcus aureus</i> (<i>S. aureus</i>) causes gastrointestinal illness worldwide. Disinfectants are used throughout the food chain for pathogenic bacteria control. We investigated <i>S. aureus</i> bioavailability in swine Mandibular lymph node tissue (MLT) and pork sausage meat (PSM), established susceptibility values for <i>S. aureus</i> to disinfectants, and determined the multilocus sequence type of MRSA strains. Antimicrobial and disinfectant susceptibility profiles were determined for 164 <i>S. aureus</i> strains isolated from swine feces (<i>n</i> = 63), MLT (<i>n</i> = 49) and PSM (<i>n</i> = 52). No antimicrobial resistance (AMR) was detected to daptomycin, nitrofurantoin, linezolid, and tigecycline, while high AMR prevalence was determined to erythromycin (50.6%), tylosin tartrate (42.7%), penicillin (72%), and tetracycline (68.9%). Methicillin-resistant <i>S. aureus</i> (MRSA) strains, ST398 (<i>n</i> = 6) and ST5 (<i>n</i> = 1), were found in the MLT and PSM, 4 MRSA in MLT and 3 MRSA strains in the PSM. About 17.5% of feces strains and 41.6% of MLT and PSM strains were resistant to chlorhexidine. All strains were susceptible to triclosan and benzalkonium chloride, with no cross-resistance between antimicrobials and disinfectants. Six MRSA strains had elevated susceptibilities to 18 disinfectants. The use of formaldehyde and tris(hydroxylmethyl)nitromethane in DC&R was not effective, which can add chemicals to the environment. Didecyldimethylammonium chloride and benzyldimethylhexadecylammonium chloride were equally effective disinfectants. ST398 and ST5 MRSA strains had elevated susceptibilities to 75% of the disinfectants tested. This study establishes susceptibility values for <i>S. aureus</i> strains from swine feces, mandibular lymph node tissue, and commercial pork sausage against 24 disinfectants. Since it was demonstrated that <i>S. aureus</i> and MRSA strains can be found deep within swine lymph node tissue, it may be beneficial for the consumer if raw swine lymph node tissue is not used in uncooked food products and pork sausage.
format	article
author	Ross C. Beier Kathleen Andrews Michael E. Hume Muhammad Umar Sohail Roger B. Harvey Toni L. Poole Tawni L. Crippen Robin C. Anderson
author_facet	Ross C. Beier Kathleen Andrews Michael E. Hume Muhammad Umar Sohail Roger B. Harvey Toni L. Poole Tawni L. Crippen Robin C. Anderson
author_sort	Ross C. Beier
title	Disinfectant and Antimicrobial Susceptibility Studies of <i>Staphylococcus aureus</i> Strains and ST398-MRSA and ST5-MRSA Strains from Swine Mandibular Lymph Node Tissue, Commercial Pork Sausage Meat and Swine Feces
title_short	Disinfectant and Antimicrobial Susceptibility Studies of <i>Staphylococcus aureus</i> Strains and ST398-MRSA and ST5-MRSA Strains from Swine Mandibular Lymph Node Tissue, Commercial Pork Sausage Meat and Swine Feces
title_full	Disinfectant and Antimicrobial Susceptibility Studies of <i>Staphylococcus aureus</i> Strains and ST398-MRSA and ST5-MRSA Strains from Swine Mandibular Lymph Node Tissue, Commercial Pork Sausage Meat and Swine Feces
title_fullStr	Disinfectant and Antimicrobial Susceptibility Studies of <i>Staphylococcus aureus</i> Strains and ST398-MRSA and ST5-MRSA Strains from Swine Mandibular Lymph Node Tissue, Commercial Pork Sausage Meat and Swine Feces
title_full_unstemmed	Disinfectant and Antimicrobial Susceptibility Studies of <i>Staphylococcus aureus</i> Strains and ST398-MRSA and ST5-MRSA Strains from Swine Mandibular Lymph Node Tissue, Commercial Pork Sausage Meat and Swine Feces
title_sort	disinfectant and antimicrobial susceptibility studies of <i>staphylococcus aureus</i> strains and st398-mrsa and st5-mrsa strains from swine mandibular lymph node tissue, commercial pork sausage meat and swine feces
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fba0ebcfa9a44a4b82f09477741d0a80
work_keys_str_mv	AT rosscbeier disinfectantandantimicrobialsusceptibilitystudiesofistaphylococcusaureusistrainsandst398mrsaandst5mrsastrainsfromswinemandibularlymphnodetissuecommercialporksausagemeatandswinefeces AT kathleenandrews disinfectantandantimicrobialsusceptibilitystudiesofistaphylococcusaureusistrainsandst398mrsaandst5mrsastrainsfromswinemandibularlymphnodetissuecommercialporksausagemeatandswinefeces AT michaelehume disinfectantandantimicrobialsusceptibilitystudiesofistaphylococcusaureusistrainsandst398mrsaandst5mrsastrainsfromswinemandibularlymphnodetissuecommercialporksausagemeatandswinefeces AT muhammadumarsohail disinfectantandantimicrobialsusceptibilitystudiesofistaphylococcusaureusistrainsandst398mrsaandst5mrsastrainsfromswinemandibularlymphnodetissuecommercialporksausagemeatandswinefeces AT rogerbharvey disinfectantandantimicrobialsusceptibilitystudiesofistaphylococcusaureusistrainsandst398mrsaandst5mrsastrainsfromswinemandibularlymphnodetissuecommercialporksausagemeatandswinefeces AT tonilpoole disinfectantandantimicrobialsusceptibilitystudiesofistaphylococcusaureusistrainsandst398mrsaandst5mrsastrainsfromswinemandibularlymphnodetissuecommercialporksausagemeatandswinefeces AT tawnilcrippen disinfectantandantimicrobialsusceptibilitystudiesofistaphylococcusaureusistrainsandst398mrsaandst5mrsastrainsfromswinemandibularlymphnodetissuecommercialporksausagemeatandswinefeces AT robincanderson disinfectantandantimicrobialsusceptibilitystudiesofistaphylococcusaureusistrainsandst398mrsaandst5mrsastrainsfromswinemandibularlymphnodetissuecommercialporksausagemeatandswinefeces
_version_	1718411236178132992

Disinfectant and Antimicrobial Susceptibility Studies of <i>Staphylococcus aureus</i> Strains and ST398-MRSA and ST5-MRSA Strains from Swine Mandibular Lymph Node Tissue, Commercial Pork Sausage Meat and Swine Feces

Ejemplares similares