New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi

ABSTRACT We have created new vectors for clustered regularly interspaced short palindromic repeat (CRISPR) mutagenesis in Candida albicans, Saccharomyces cerevisiae, Candida glabrata, and Naumovozyma castellii. These new vectors permit a comparison of the requirements for CRISPR mutagenesis in each...

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Bibliographic Details
Main Authors: Valmik K. Vyas, G. Guy Bushkin, Douglas A. Bernstein, Matthew A. Getz, Magdalena Sewastianik, M. Inmaculada Barrasa, David P. Bartel, Gerald R. Fink
Format: article
Language:EN
Published: American Society for Microbiology 2018
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Online Access:https://doaj.org/article/fc3e689ff07143b294c2db074a0e1a1e
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