Prevalence and Characteristics of Extended-Spectrum-β-Lactamase-Producing and Carbapenemase-Producing <italic toggle="yes">Enterobacteriaceae</italic> from Freshwater Fish and Pork in Wet Markets of Hong Kong

ABSTRACT This study identified and characterized extended-spectrum-β-lactamase-producing Enterobacteriaceae (ESBL-E) and carbapenemase-producing Enterobacteriaceae (CPE) from farmed freshwater fish and pig offal procured from the wet markets across Hong Kong. During March 2018 to January 2019, 730 f...

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Autores principales:	Dulmini Nanayakkara Sapugahawatte, Carmen Li, Chendi Zhu, Priyanga Dharmaratne, Kam Tak Wong, Norman Lo, Margaret Ip
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	ESBL CPE Enterobacteriaceae freshwater fish pigs livestock Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fc5171bb09a74554b761c93edff0fe01
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spelling	oai:doaj.org-article:fc5171bb09a74554b761c93edff0fe012021-11-15T15:29:16ZPrevalence and Characteristics of Extended-Spectrum-β-Lactamase-Producing and Carbapenemase-Producing <italic toggle="yes">Enterobacteriaceae</italic> from Freshwater Fish and Pork in Wet Markets of Hong Kong10.1128/mSphere.00107-202379-5042https://doaj.org/article/fc5171bb09a74554b761c93edff0fe012020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00107-20https://doaj.org/toc/2379-5042ABSTRACT This study identified and characterized extended-spectrum-β-lactamase-producing Enterobacteriaceae (ESBL-E) and carbapenemase-producing Enterobacteriaceae (CPE) from farmed freshwater fish and pig offal procured from the wet markets across Hong Kong. During March 2018 to January 2019, 730 food animal samples, namely, 213 snakehead fish, 198 black carp, and 339 pig organs, were examined. ESBL-E and CPE were isolated from the homogenized samples plated on selective media and identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF-MS). All ESBL-E and CPE strains were tested for antimicrobial susceptibilities. ESBL-E and CPE gene groups were detected by multiplex PCR and blaCTX-M-1/-2/-9 group strains were Sanger sequenced for CTX-M types. All CPE isolates were whole-genome sequenced. Isolation of ESBL-E from pig small (52.4%) and large (50%) intestines and tongues (25.1%) was significantly (P < 0.05) more frequent than from snakehead (0.94%) and black carp (0.5%) fish. ESBL-E isolates (n = 171) revealed resistance rates of 16.3%, 29.8%, 35.6%, 53.2%, 55.0%, and 100% to piperacillin-tazobactam, amoxicillin-clavulanate, cefepime, gentamicin, ciprofloxacin, and ampicillin, respectively, whereas CPE (n = 28) were resistant to almost all the antibiotics tested except gentamicin, ciprofloxacin, and fosfomycin. The predominant ESBL gene groups in fishes and pig offals were blaCTX, where blaCTX-M-55 was the major subtype in the blaCTX-M-1 group (64.4% of isolates in the group). blaCTX-M-14/-17 was the major genotype in the blaCTX-M-9 group (32.2%). All CPE strains possessed blaNDM genes. High rates of ESBL-E and CPE were identified in food animals from wet markets of Hong Kong, which may serve as a potential reservoir of antimicrobial-resistant genes and increase the challenges in tackling antimicrobial resistance beyond health care settings. IMPORTANCE Extended-spectrum-β-lactamase-producing Enterobacteriaceae (ESBL-E) and carbapenemase-producing Enterobacteriaceae (CPE) are of global health importance, yet there is a paucity of surveillance studies on food animals in Hong Kong. Here, we report a high prevalence of ESBL-E (ranging from 0.5% to 52.4%) and CPE (0% to 9.9%) from various food animal samples procured from wet markets across Hong Kong. All CPE strains were characterized by whole-genome sequencing and possessed NDM-1 and -5 genes and other resistance determinants. Given the increased resistance profile of these strains, this study highlights the emerging threat of ESBL-E and CPE disseminated in farmed animals. Furthermore, our data enriched our understanding of antibiotic resistance reservoirs from a One Health perspective that can widely spread across various niches, beyond health care settings.Dulmini Nanayakkara SapugahawatteCarmen LiChendi ZhuPriyanga DharmaratneKam Tak WongNorman LoMargaret IpAmerican Society for MicrobiologyarticleESBLCPEEnterobacteriaceaefreshwater fishpigslivestockMicrobiologyQR1-502ENmSphere, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	ESBL CPE Enterobacteriaceae freshwater fish pigs livestock Microbiology QR1-502
spellingShingle	ESBL CPE Enterobacteriaceae freshwater fish pigs livestock Microbiology QR1-502 Dulmini Nanayakkara Sapugahawatte Carmen Li Chendi Zhu Priyanga Dharmaratne Kam Tak Wong Norman Lo Margaret Ip Prevalence and Characteristics of Extended-Spectrum-β-Lactamase-Producing and Carbapenemase-Producing <italic toggle="yes">Enterobacteriaceae</italic> from Freshwater Fish and Pork in Wet Markets of Hong Kong
description	ABSTRACT This study identified and characterized extended-spectrum-β-lactamase-producing Enterobacteriaceae (ESBL-E) and carbapenemase-producing Enterobacteriaceae (CPE) from farmed freshwater fish and pig offal procured from the wet markets across Hong Kong. During March 2018 to January 2019, 730 food animal samples, namely, 213 snakehead fish, 198 black carp, and 339 pig organs, were examined. ESBL-E and CPE were isolated from the homogenized samples plated on selective media and identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF-MS). All ESBL-E and CPE strains were tested for antimicrobial susceptibilities. ESBL-E and CPE gene groups were detected by multiplex PCR and blaCTX-M-1/-2/-9 group strains were Sanger sequenced for CTX-M types. All CPE isolates were whole-genome sequenced. Isolation of ESBL-E from pig small (52.4%) and large (50%) intestines and tongues (25.1%) was significantly (P < 0.05) more frequent than from snakehead (0.94%) and black carp (0.5%) fish. ESBL-E isolates (n = 171) revealed resistance rates of 16.3%, 29.8%, 35.6%, 53.2%, 55.0%, and 100% to piperacillin-tazobactam, amoxicillin-clavulanate, cefepime, gentamicin, ciprofloxacin, and ampicillin, respectively, whereas CPE (n = 28) were resistant to almost all the antibiotics tested except gentamicin, ciprofloxacin, and fosfomycin. The predominant ESBL gene groups in fishes and pig offals were blaCTX, where blaCTX-M-55 was the major subtype in the blaCTX-M-1 group (64.4% of isolates in the group). blaCTX-M-14/-17 was the major genotype in the blaCTX-M-9 group (32.2%). All CPE strains possessed blaNDM genes. High rates of ESBL-E and CPE were identified in food animals from wet markets of Hong Kong, which may serve as a potential reservoir of antimicrobial-resistant genes and increase the challenges in tackling antimicrobial resistance beyond health care settings. IMPORTANCE Extended-spectrum-β-lactamase-producing Enterobacteriaceae (ESBL-E) and carbapenemase-producing Enterobacteriaceae (CPE) are of global health importance, yet there is a paucity of surveillance studies on food animals in Hong Kong. Here, we report a high prevalence of ESBL-E (ranging from 0.5% to 52.4%) and CPE (0% to 9.9%) from various food animal samples procured from wet markets across Hong Kong. All CPE strains were characterized by whole-genome sequencing and possessed NDM-1 and -5 genes and other resistance determinants. Given the increased resistance profile of these strains, this study highlights the emerging threat of ESBL-E and CPE disseminated in farmed animals. Furthermore, our data enriched our understanding of antibiotic resistance reservoirs from a One Health perspective that can widely spread across various niches, beyond health care settings.
format	article
author	Dulmini Nanayakkara Sapugahawatte Carmen Li Chendi Zhu Priyanga Dharmaratne Kam Tak Wong Norman Lo Margaret Ip
author_facet	Dulmini Nanayakkara Sapugahawatte Carmen Li Chendi Zhu Priyanga Dharmaratne Kam Tak Wong Norman Lo Margaret Ip
author_sort	Dulmini Nanayakkara Sapugahawatte
title	Prevalence and Characteristics of Extended-Spectrum-β-Lactamase-Producing and Carbapenemase-Producing <italic toggle="yes">Enterobacteriaceae</italic> from Freshwater Fish and Pork in Wet Markets of Hong Kong
title_short	Prevalence and Characteristics of Extended-Spectrum-β-Lactamase-Producing and Carbapenemase-Producing <italic toggle="yes">Enterobacteriaceae</italic> from Freshwater Fish and Pork in Wet Markets of Hong Kong
title_full	Prevalence and Characteristics of Extended-Spectrum-β-Lactamase-Producing and Carbapenemase-Producing <italic toggle="yes">Enterobacteriaceae</italic> from Freshwater Fish and Pork in Wet Markets of Hong Kong
title_fullStr	Prevalence and Characteristics of Extended-Spectrum-β-Lactamase-Producing and Carbapenemase-Producing <italic toggle="yes">Enterobacteriaceae</italic> from Freshwater Fish and Pork in Wet Markets of Hong Kong
title_full_unstemmed	Prevalence and Characteristics of Extended-Spectrum-β-Lactamase-Producing and Carbapenemase-Producing <italic toggle="yes">Enterobacteriaceae</italic> from Freshwater Fish and Pork in Wet Markets of Hong Kong
title_sort	prevalence and characteristics of extended-spectrum-β-lactamase-producing and carbapenemase-producing <italic toggle="yes">enterobacteriaceae</italic> from freshwater fish and pork in wet markets of hong kong
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/fc5171bb09a74554b761c93edff0fe01
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Prevalence and Characteristics of Extended-Spectrum-β-Lactamase-Producing and Carbapenemase-Producing <italic toggle="yes">Enterobacteriaceae</italic> from Freshwater Fish and Pork in Wet Markets of Hong Kong

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