Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations

Abstract Elucidating the amino acid (AA) metabolism patterns of Streptococcus thermophilus has important effects on the precise design of nitrogen sources for high-cell-density culture. Transcriptomics and metabolomics were combined to reveal the cysteine, methionine, glutamate, glutamine, arginine,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yali Qiao, Gefei Liu, Cong Leng, Yanjiao Zhang, Xuepeng Lv, Hongyu Chen, Jiahui Sun, Zhen Feng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc6acefc1d82433ab8d15985397f6f6c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fc6acefc1d82433ab8d15985397f6f6c
record_format	dspace
spelling	oai:doaj.org-article:fc6acefc1d82433ab8d15985397f6f6c2021-12-02T15:08:01ZMetabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations10.1038/s41598-018-30272-52045-2322https://doaj.org/article/fc6acefc1d82433ab8d15985397f6f6c2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30272-5https://doaj.org/toc/2045-2322Abstract Elucidating the amino acid (AA) metabolism patterns of Streptococcus thermophilus has important effects on the precise design of nitrogen sources for high-cell-density culture. Transcriptomics and metabolomics were combined to reveal the cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine and alanine metabolic pathways in S. thermophilus MN-ZLW-002, including glutathione. The changes in the synthesis, consumption and concentration of AAs and their metabolites, as well as regulatory genes with time were revealed. The metabolism of L-cysteine, L-glutamate, L-aspartate and L-alanine generated some potential functional metabolites. The metabolism of methionine and glutamate generated potential harmful metabolites. S. thermophilus MN-ZLW-002 can synthesize glutathione. Some potential functional metabolites have similar biological functions, indicating that S. thermophilus can resist environmental stresses through multiple mechanisms. The expression of some key genes in synthesis pathway of AA indicated that cysteine, methionine, asparagine, aspartate, arginine and lysine were insufficient or imbalance between nutrient components. The accumulation of large amounts of AA metabolites might be the primary cause of the overconsumption of AAs and influence the growth of S. thermophilus. The present study revealed the metabolic profiles of abovementioned AAs as well as those of regulatory genes and metabolites. These results were beneficial to the precise design of nitrogen sources and regulation of functional metabolites for the high-cell-density culture of S. thermophilus.Yali QiaoGefei LiuCong LengYanjiao ZhangXuepeng LvHongyu ChenJiahui SunZhen FengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yali Qiao Gefei Liu Cong Leng Yanjiao Zhang Xuepeng Lv Hongyu Chen Jiahui Sun Zhen Feng Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations
description	Abstract Elucidating the amino acid (AA) metabolism patterns of Streptococcus thermophilus has important effects on the precise design of nitrogen sources for high-cell-density culture. Transcriptomics and metabolomics were combined to reveal the cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine and alanine metabolic pathways in S. thermophilus MN-ZLW-002, including glutathione. The changes in the synthesis, consumption and concentration of AAs and their metabolites, as well as regulatory genes with time were revealed. The metabolism of L-cysteine, L-glutamate, L-aspartate and L-alanine generated some potential functional metabolites. The metabolism of methionine and glutamate generated potential harmful metabolites. S. thermophilus MN-ZLW-002 can synthesize glutathione. Some potential functional metabolites have similar biological functions, indicating that S. thermophilus can resist environmental stresses through multiple mechanisms. The expression of some key genes in synthesis pathway of AA indicated that cysteine, methionine, asparagine, aspartate, arginine and lysine were insufficient or imbalance between nutrient components. The accumulation of large amounts of AA metabolites might be the primary cause of the overconsumption of AAs and influence the growth of S. thermophilus. The present study revealed the metabolic profiles of abovementioned AAs as well as those of regulatory genes and metabolites. These results were beneficial to the precise design of nitrogen sources and regulation of functional metabolites for the high-cell-density culture of S. thermophilus.
format	article
author	Yali Qiao Gefei Liu Cong Leng Yanjiao Zhang Xuepeng Lv Hongyu Chen Jiahui Sun Zhen Feng
author_facet	Yali Qiao Gefei Liu Cong Leng Yanjiao Zhang Xuepeng Lv Hongyu Chen Jiahui Sun Zhen Feng
author_sort	Yali Qiao
title	Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations
title_short	Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations
title_full	Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations
title_fullStr	Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations
title_full_unstemmed	Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations
title_sort	metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in streptococcus thermophilus during ph-controlled batch fermentations
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/fc6acefc1d82433ab8d15985397f6f6c
work_keys_str_mv	AT yaliqiao metabolicprofilesofcysteinemethionineglutamateglutaminearginineaspartateasparaginealanineandglutathioneinstreptococcusthermophilusduringphcontrolledbatchfermentations AT gefeiliu metabolicprofilesofcysteinemethionineglutamateglutaminearginineaspartateasparaginealanineandglutathioneinstreptococcusthermophilusduringphcontrolledbatchfermentations AT congleng metabolicprofilesofcysteinemethionineglutamateglutaminearginineaspartateasparaginealanineandglutathioneinstreptococcusthermophilusduringphcontrolledbatchfermentations AT yanjiaozhang metabolicprofilesofcysteinemethionineglutamateglutaminearginineaspartateasparaginealanineandglutathioneinstreptococcusthermophilusduringphcontrolledbatchfermentations AT xuepenglv metabolicprofilesofcysteinemethionineglutamateglutaminearginineaspartateasparaginealanineandglutathioneinstreptococcusthermophilusduringphcontrolledbatchfermentations AT hongyuchen metabolicprofilesofcysteinemethionineglutamateglutaminearginineaspartateasparaginealanineandglutathioneinstreptococcusthermophilusduringphcontrolledbatchfermentations AT jiahuisun metabolicprofilesofcysteinemethionineglutamateglutaminearginineaspartateasparaginealanineandglutathioneinstreptococcusthermophilusduringphcontrolledbatchfermentations AT zhenfeng metabolicprofilesofcysteinemethionineglutamateglutaminearginineaspartateasparaginealanineandglutathioneinstreptococcusthermophilusduringphcontrolledbatchfermentations
_version_	1718388360752398336

Metabolic profiles of cysteine, methionine, glutamate, glutamine, arginine, aspartate, asparagine, alanine and glutathione in Streptococcus thermophilus during pH-controlled batch fermentations

Ejemplares similares