Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds

Leucaena leucocephala (lmk.) de Wit) (leed tree) seeds have been fermented by Indonesian as a traditional food which is called tempeh. The fermentation products of legume seeds are good functional foods, since they contain many bioactive compounds. The objective of this study was to determine the bi...

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Autores principales:	Venty Suryanti, Soerya Dewi Marliyana, Gracia Lasma Rohana, Elyna Wahyu Trisnawati, Widiyanti Widiyanti
Formato:	article
Lenguaje:	EN ID
Publicado:	Jenderal Soedirman University 2021
Materias:	Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/fcf4da190e53402e82c0d63c9da91f44
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spelling	oai:doaj.org-article:fcf4da190e53402e82c0d63c9da91f442021-11-22T01:13:54ZBioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds1907-97612503-031010.20884/1.jm.2021.16.3.756https://doaj.org/article/fcf4da190e53402e82c0d63c9da91f442021-11-01T00:00:00Zhttps://ojs.jmolekul.com/ojs/index.php/jm/article/view/756https://doaj.org/toc/1907-9761https://doaj.org/toc/2503-0310Leucaena leucocephala (lmk.) de Wit) (leed tree) seeds have been fermented by Indonesian as a traditional food which is called tempeh. The fermentation products of legume seeds are good functional foods, since they contain many bioactive compounds. The objective of this study was to determine the bioactive compound contents of fermented lead tree seed, such as total phenolic, ascorbic acid, β-carotene and a-tocopherol. Antioxidant activity testing was carried out using the thiobarbituric acid reactive substances (TBARS) method. Analysis of total phenolics, ascorbic acid, β-carotene and a-tocopherol were carried out using the folin-ciocalteu method, iodine titration, spectrophotometry and iron (III) chloride-thiocyanate, respectively. Rhizopus oligosporus was used to ferment lead tree seeds for 0, 1, 2, 3 and 4 days. Fermented leed tree seed powder was extracted with petroleum ether followed by ethanol. In comparison to unfermented leed tree seed powder, the β-carotene and a-tocopherol of the fermented seed powder increased. The greatest enhancement of β-carotene and a-tocopherol contents were observed at day 2 of fermentation. The highest antioxidant activities of fermented leed tree seeds were also obained at day 2 of fermentation with 55.55±4.59 and 74.46±3.49% inhibition in petroleum ether and ethanol extracts, respectively. The results show that lead tree seed tempeh has high antioxidant activity due to the bioactive compounds contains, such as phenolics, β-carotene and a-tocopherol. Therefore, leed tree seed tempeh could be used for possible commercial production of functional food to alleviate oxidative stress.Venty SuryantiSoerya Dewi MarliyanaGracia Lasma RohanaElyna Wahyu TrisnawatiWidiyanti WidiyantiJenderal Soedirman UniversityarticleChemistryQD1-999ENIDMolekul, Vol 16, Iss 3, Pp 192-199 (2021)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	Chemistry QD1-999
spellingShingle	Chemistry QD1-999 Venty Suryanti Soerya Dewi Marliyana Gracia Lasma Rohana Elyna Wahyu Trisnawati Widiyanti Widiyanti Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds
description	Leucaena leucocephala (lmk.) de Wit) (leed tree) seeds have been fermented by Indonesian as a traditional food which is called tempeh. The fermentation products of legume seeds are good functional foods, since they contain many bioactive compounds. The objective of this study was to determine the bioactive compound contents of fermented lead tree seed, such as total phenolic, ascorbic acid, β-carotene and a-tocopherol. Antioxidant activity testing was carried out using the thiobarbituric acid reactive substances (TBARS) method. Analysis of total phenolics, ascorbic acid, β-carotene and a-tocopherol were carried out using the folin-ciocalteu method, iodine titration, spectrophotometry and iron (III) chloride-thiocyanate, respectively. Rhizopus oligosporus was used to ferment lead tree seeds for 0, 1, 2, 3 and 4 days. Fermented leed tree seed powder was extracted with petroleum ether followed by ethanol. In comparison to unfermented leed tree seed powder, the β-carotene and a-tocopherol of the fermented seed powder increased. The greatest enhancement of β-carotene and a-tocopherol contents were observed at day 2 of fermentation. The highest antioxidant activities of fermented leed tree seeds were also obained at day 2 of fermentation with 55.55±4.59 and 74.46±3.49% inhibition in petroleum ether and ethanol extracts, respectively. The results show that lead tree seed tempeh has high antioxidant activity due to the bioactive compounds contains, such as phenolics, β-carotene and a-tocopherol. Therefore, leed tree seed tempeh could be used for possible commercial production of functional food to alleviate oxidative stress.
format	article
author	Venty Suryanti Soerya Dewi Marliyana Gracia Lasma Rohana Elyna Wahyu Trisnawati Widiyanti Widiyanti
author_facet	Venty Suryanti Soerya Dewi Marliyana Gracia Lasma Rohana Elyna Wahyu Trisnawati Widiyanti Widiyanti
author_sort	Venty Suryanti
title	Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds
title_short	Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds
title_full	Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds
title_fullStr	Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds
title_full_unstemmed	Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds
title_sort	bioactive compound contents and antioxidant activity of of fermented lead tree (leucaena leucocephala (lmk.) de wit) seeds
publisher	Jenderal Soedirman University
publishDate	2021
url	https://doaj.org/article/fcf4da190e53402e82c0d63c9da91f44
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Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds

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