Characterization of a Novel Mitovirus Infecting Melanconiella theae Isolated From Tea Plants
A dsRNA segment was identified in the fungus Melanconiella theae isolated from tea plants. The complete dsRNA sequence, determined by random cloning together with RACE protocol, is 2,461 bp in length with an AU-rich content (62.37%) and comprises a single ORF of 2,265-nucleotides encoding an RNA-dep...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:fde4bea9c5f14ecb97207f1ebf0d9a272021-11-17T06:36:41ZCharacterization of a Novel Mitovirus Infecting Melanconiella theae Isolated From Tea Plants1664-302X10.3389/fmicb.2021.757556https://doaj.org/article/fde4bea9c5f14ecb97207f1ebf0d9a272021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.757556/fullhttps://doaj.org/toc/1664-302XA dsRNA segment was identified in the fungus Melanconiella theae isolated from tea plants. The complete dsRNA sequence, determined by random cloning together with RACE protocol, is 2,461 bp in length with an AU-rich content (62.37%) and comprises a single ORF of 2,265-nucleotides encoding an RNA-dependent RNA-polymerase (RdRp, 754 amino acids in size). The terminus sequences can fold into predicted stable stem-loop structures. A BLASTX and phylogenetic analysis revealed the dsRNA genome shows similarities with the RdRp sequences of mitoviruses, with the highest identity of 48% with those of grapevine-associated mitovirus 20 and Colletotrichum fructicola mitovirus 1. Our results reveal a novel member, tentatively named Melanconiella theae mitovirus 1 (MtMV1), belongs to the family Mitoviridae. MtMV1 is capsidless as examined by transmission electron microscope, efficiently transmitted through conidia as 100 conidium-generated colonies were analyzed, and easily eliminated by hyphal tipping method combined with green-leaf tea powder. MtMV1 has a genomic sequence obviously divergent from those of most members in the family Mitoviridae and some unique characteristics unreported in known members. This is the first report of a mycovirus infecting Melanconiella fungi to date.Karim ShafikKarim ShafikKarim ShafikKarim ShafikKarim ShafikMuhammad UmerMuhammad UmerMuhammad UmerMuhammad UmerHuafeng YouHuafeng YouHuafeng YouHuafeng YouHamdy AboushedidaHamdy AboushedidaHamdy AboushedidaHamdy AboushedidaHamdy AboushedidaZhenhua WangDejiang NiWenxing XuWenxing XuWenxing XuWenxing XuFrontiers Media S.A.articlemycovirusmitovirusmitochondrial virusMtMV1Melanconiella theaeCamellia sinensisMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021) |
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DOAJ |
| language |
EN |
| topic |
mycovirus mitovirus mitochondrial virus MtMV1 Melanconiella theae Camellia sinensis Microbiology QR1-502 |
| spellingShingle |
mycovirus mitovirus mitochondrial virus MtMV1 Melanconiella theae Camellia sinensis Microbiology QR1-502 Karim Shafik Karim Shafik Karim Shafik Karim Shafik Karim Shafik Muhammad Umer Muhammad Umer Muhammad Umer Muhammad Umer Huafeng You Huafeng You Huafeng You Huafeng You Hamdy Aboushedida Hamdy Aboushedida Hamdy Aboushedida Hamdy Aboushedida Hamdy Aboushedida Zhenhua Wang Dejiang Ni Wenxing Xu Wenxing Xu Wenxing Xu Wenxing Xu Characterization of a Novel Mitovirus Infecting Melanconiella theae Isolated From Tea Plants |
| description |
A dsRNA segment was identified in the fungus Melanconiella theae isolated from tea plants. The complete dsRNA sequence, determined by random cloning together with RACE protocol, is 2,461 bp in length with an AU-rich content (62.37%) and comprises a single ORF of 2,265-nucleotides encoding an RNA-dependent RNA-polymerase (RdRp, 754 amino acids in size). The terminus sequences can fold into predicted stable stem-loop structures. A BLASTX and phylogenetic analysis revealed the dsRNA genome shows similarities with the RdRp sequences of mitoviruses, with the highest identity of 48% with those of grapevine-associated mitovirus 20 and Colletotrichum fructicola mitovirus 1. Our results reveal a novel member, tentatively named Melanconiella theae mitovirus 1 (MtMV1), belongs to the family Mitoviridae. MtMV1 is capsidless as examined by transmission electron microscope, efficiently transmitted through conidia as 100 conidium-generated colonies were analyzed, and easily eliminated by hyphal tipping method combined with green-leaf tea powder. MtMV1 has a genomic sequence obviously divergent from those of most members in the family Mitoviridae and some unique characteristics unreported in known members. This is the first report of a mycovirus infecting Melanconiella fungi to date. |
| format |
article |
| author |
Karim Shafik Karim Shafik Karim Shafik Karim Shafik Karim Shafik Muhammad Umer Muhammad Umer Muhammad Umer Muhammad Umer Huafeng You Huafeng You Huafeng You Huafeng You Hamdy Aboushedida Hamdy Aboushedida Hamdy Aboushedida Hamdy Aboushedida Hamdy Aboushedida Zhenhua Wang Dejiang Ni Wenxing Xu Wenxing Xu Wenxing Xu Wenxing Xu |
| author_facet |
Karim Shafik Karim Shafik Karim Shafik Karim Shafik Karim Shafik Muhammad Umer Muhammad Umer Muhammad Umer Muhammad Umer Huafeng You Huafeng You Huafeng You Huafeng You Hamdy Aboushedida Hamdy Aboushedida Hamdy Aboushedida Hamdy Aboushedida Hamdy Aboushedida Zhenhua Wang Dejiang Ni Wenxing Xu Wenxing Xu Wenxing Xu Wenxing Xu |
| author_sort |
Karim Shafik |
| title |
Characterization of a Novel Mitovirus Infecting Melanconiella theae Isolated From Tea Plants |
| title_short |
Characterization of a Novel Mitovirus Infecting Melanconiella theae Isolated From Tea Plants |
| title_full |
Characterization of a Novel Mitovirus Infecting Melanconiella theae Isolated From Tea Plants |
| title_fullStr |
Characterization of a Novel Mitovirus Infecting Melanconiella theae Isolated From Tea Plants |
| title_full_unstemmed |
Characterization of a Novel Mitovirus Infecting Melanconiella theae Isolated From Tea Plants |
| title_sort |
characterization of a novel mitovirus infecting melanconiella theae isolated from tea plants |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/fde4bea9c5f14ecb97207f1ebf0d9a27 |
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