Natural Products Self-Assembled Nanozyme for Cascade Detection of Glucose and Bacterial Viability in Food
Sugar content and bacterial contamination levels are important indicators for the health and safety of food, respectively. Therefore, it is important to construct a method that can detect both glucose and bacterial activity. Herein, natural compounds (gallic acid and glucose oxidase) were assembled...
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MDPI AG
2021
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oai:doaj.org-article:fe491f08aaad4a0489be4af366c365332021-11-25T17:33:11ZNatural Products Self-Assembled Nanozyme for Cascade Detection of Glucose and Bacterial Viability in Food10.3390/foods101125962304-8158https://doaj.org/article/fe491f08aaad4a0489be4af366c365332021-10-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2596https://doaj.org/toc/2304-8158Sugar content and bacterial contamination levels are important indicators for the health and safety of food, respectively. Therefore, it is important to construct a method that can detect both glucose and bacterial activity. Herein, natural compounds (gallic acid and glucose oxidase) were assembled into nanozyme (GOx@GA-Fe (ii)) for mild cascade detection. The nanozyme catalyzes glucose to produce hydrogen peroxide, which is then converted to ·OH and oxidized colorless TMB from blue oxidized TMB. Under the optimal conditions, the method has a good linear relationship in the glucose concentration range of 1–500 μM (R<sup>2</sup> = 0.993) with minimum detection concentration of 0.43 μM. Based on the glucose consumption of bacteria metabolism, the cascade reaction was then applied to detect the viability of 5 common bacteria. As such, a cascade method based on a natural self-assembled nanozyme was fabricated to monitor the quality of food.Qiuping ZhangXinze WangYi KangHao SunYanmin LiangJie LiuZehui SuJie DanLinpin LuoTianli YueJianlong WangWentao ZhangMDPI AGarticlenanozymecascade reactionglucose concentrationbacterial viabilityChemical technologyTP1-1185ENFoods, Vol 10, Iss 2596, p 2596 (2021) |
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DOAJ |
| collection |
DOAJ |
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| topic |
nanozyme cascade reaction glucose concentration bacterial viability Chemical technology TP1-1185 |
| spellingShingle |
nanozyme cascade reaction glucose concentration bacterial viability Chemical technology TP1-1185 Qiuping Zhang Xinze Wang Yi Kang Hao Sun Yanmin Liang Jie Liu Zehui Su Jie Dan Linpin Luo Tianli Yue Jianlong Wang Wentao Zhang Natural Products Self-Assembled Nanozyme for Cascade Detection of Glucose and Bacterial Viability in Food |
| description |
Sugar content and bacterial contamination levels are important indicators for the health and safety of food, respectively. Therefore, it is important to construct a method that can detect both glucose and bacterial activity. Herein, natural compounds (gallic acid and glucose oxidase) were assembled into nanozyme (GOx@GA-Fe (ii)) for mild cascade detection. The nanozyme catalyzes glucose to produce hydrogen peroxide, which is then converted to ·OH and oxidized colorless TMB from blue oxidized TMB. Under the optimal conditions, the method has a good linear relationship in the glucose concentration range of 1–500 μM (R<sup>2</sup> = 0.993) with minimum detection concentration of 0.43 μM. Based on the glucose consumption of bacteria metabolism, the cascade reaction was then applied to detect the viability of 5 common bacteria. As such, a cascade method based on a natural self-assembled nanozyme was fabricated to monitor the quality of food. |
| format |
article |
| author |
Qiuping Zhang Xinze Wang Yi Kang Hao Sun Yanmin Liang Jie Liu Zehui Su Jie Dan Linpin Luo Tianli Yue Jianlong Wang Wentao Zhang |
| author_facet |
Qiuping Zhang Xinze Wang Yi Kang Hao Sun Yanmin Liang Jie Liu Zehui Su Jie Dan Linpin Luo Tianli Yue Jianlong Wang Wentao Zhang |
| author_sort |
Qiuping Zhang |
| title |
Natural Products Self-Assembled Nanozyme for Cascade Detection of Glucose and Bacterial Viability in Food |
| title_short |
Natural Products Self-Assembled Nanozyme for Cascade Detection of Glucose and Bacterial Viability in Food |
| title_full |
Natural Products Self-Assembled Nanozyme for Cascade Detection of Glucose and Bacterial Viability in Food |
| title_fullStr |
Natural Products Self-Assembled Nanozyme for Cascade Detection of Glucose and Bacterial Viability in Food |
| title_full_unstemmed |
Natural Products Self-Assembled Nanozyme for Cascade Detection of Glucose and Bacterial Viability in Food |
| title_sort |
natural products self-assembled nanozyme for cascade detection of glucose and bacterial viability in food |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/fe491f08aaad4a0489be4af366c36533 |
| work_keys_str_mv |
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