The Role of Ancestral Duplicated Genes in Adaptation to Growth on Lactate, a Non-Fermentable Carbon Source for the Yeast <em>Saccharomyces cerevisiae</em>

The cell central metabolism has been shaped throughout evolutionary times when facing challenges from the availability of resources. In the budding yeast, <i>Saccharomyces cerevisiae</i>, a set of duplicated genes originating from an ancestral whole-genome and several coetaneous small-sc...

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Autores principales:	Florian Mattenberger, Mario A. Fares, Christina Toft, Beatriz Sabater-Muñoz
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	whole-genome duplicates small-scale duplicates phenotypic response metabolic distance acidic stress reactive oxygen response Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/fed7fe5371514672a83fac69999f03bc
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spelling	oai:doaj.org-article:fed7fe5371514672a83fac69999f03bc2021-11-25T17:55:09ZThe Role of Ancestral Duplicated Genes in Adaptation to Growth on Lactate, a Non-Fermentable Carbon Source for the Yeast <em>Saccharomyces cerevisiae</em>10.3390/ijms2222122931422-00671661-6596https://doaj.org/article/fed7fe5371514672a83fac69999f03bc2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12293https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The cell central metabolism has been shaped throughout evolutionary times when facing challenges from the availability of resources. In the budding yeast, <i>Saccharomyces cerevisiae</i>, a set of duplicated genes originating from an ancestral whole-genome and several coetaneous small-scale duplication events drive energy transfer through glucose metabolism as the main carbon source either by fermentation or respiration. These duplicates (~a third of the genome) have been dated back to approximately 100 MY, allowing for enough evolutionary time to diverge in both sequence and function. Gene duplication has been proposed as a molecular mechanism of biological innovation, maintaining balance between mutational robustness and evolvability of the system. However, some questions concerning the molecular mechanisms behind duplicated genes transcriptional plasticity and functional divergence remain unresolved. In this work we challenged <i>S. cerevisiae</i> to the use of lactic acid/lactate as the sole carbon source and performed a small adaptive laboratory evolution to this non-fermentative carbon source, determining phenotypic and transcriptomic changes. We observed growth adaptation to acidic stress, by reduction of growth rate and increase in biomass production, while the transcriptomic response was mainly driven by repression of the whole-genome duplicates, those implied in glycolysis and overexpression of ROS response. The contribution of several duplicated pairs to this carbon source switch and acidic stress is also discussed.Florian MattenbergerMario A. FaresChristina ToftBeatriz Sabater-MuñozMDPI AGarticlewhole-genome duplicatessmall-scale duplicatesphenotypic responsemetabolic distanceacidic stressreactive oxygen responseBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12293, p 12293 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	whole-genome duplicates small-scale duplicates phenotypic response metabolic distance acidic stress reactive oxygen response Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	whole-genome duplicates small-scale duplicates phenotypic response metabolic distance acidic stress reactive oxygen response Biology (General) QH301-705.5 Chemistry QD1-999 Florian Mattenberger Mario A. Fares Christina Toft Beatriz Sabater-Muñoz The Role of Ancestral Duplicated Genes in Adaptation to Growth on Lactate, a Non-Fermentable Carbon Source for the Yeast <em>Saccharomyces cerevisiae</em>
description	The cell central metabolism has been shaped throughout evolutionary times when facing challenges from the availability of resources. In the budding yeast, <i>Saccharomyces cerevisiae</i>, a set of duplicated genes originating from an ancestral whole-genome and several coetaneous small-scale duplication events drive energy transfer through glucose metabolism as the main carbon source either by fermentation or respiration. These duplicates (~a third of the genome) have been dated back to approximately 100 MY, allowing for enough evolutionary time to diverge in both sequence and function. Gene duplication has been proposed as a molecular mechanism of biological innovation, maintaining balance between mutational robustness and evolvability of the system. However, some questions concerning the molecular mechanisms behind duplicated genes transcriptional plasticity and functional divergence remain unresolved. In this work we challenged <i>S. cerevisiae</i> to the use of lactic acid/lactate as the sole carbon source and performed a small adaptive laboratory evolution to this non-fermentative carbon source, determining phenotypic and transcriptomic changes. We observed growth adaptation to acidic stress, by reduction of growth rate and increase in biomass production, while the transcriptomic response was mainly driven by repression of the whole-genome duplicates, those implied in glycolysis and overexpression of ROS response. The contribution of several duplicated pairs to this carbon source switch and acidic stress is also discussed.
format	article
author	Florian Mattenberger Mario A. Fares Christina Toft Beatriz Sabater-Muñoz
author_facet	Florian Mattenberger Mario A. Fares Christina Toft Beatriz Sabater-Muñoz
author_sort	Florian Mattenberger
title	The Role of Ancestral Duplicated Genes in Adaptation to Growth on Lactate, a Non-Fermentable Carbon Source for the Yeast <em>Saccharomyces cerevisiae</em>
title_short	The Role of Ancestral Duplicated Genes in Adaptation to Growth on Lactate, a Non-Fermentable Carbon Source for the Yeast <em>Saccharomyces cerevisiae</em>
title_full	The Role of Ancestral Duplicated Genes in Adaptation to Growth on Lactate, a Non-Fermentable Carbon Source for the Yeast <em>Saccharomyces cerevisiae</em>
title_fullStr	The Role of Ancestral Duplicated Genes in Adaptation to Growth on Lactate, a Non-Fermentable Carbon Source for the Yeast <em>Saccharomyces cerevisiae</em>
title_full_unstemmed	The Role of Ancestral Duplicated Genes in Adaptation to Growth on Lactate, a Non-Fermentable Carbon Source for the Yeast <em>Saccharomyces cerevisiae</em>
title_sort	role of ancestral duplicated genes in adaptation to growth on lactate, a non-fermentable carbon source for the yeast <em>saccharomyces cerevisiae</em>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fed7fe5371514672a83fac69999f03bc
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The Role of Ancestral Duplicated Genes in Adaptation to Growth on Lactate, a Non-Fermentable Carbon Source for the Yeast <em>Saccharomyces cerevisiae</em>

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