Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution

Precision design of DNA origami needs precision validation. Here, the authors developed cryo-EM methods for obtaining high resolution structural data and for constructing pseudo-atomic models in a semi-automated fashion, allowing for iterative nanodevice inspection and refinement.

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Autores principales:	Massimo Kube, Fabian Kohler, Elija Feigl, Baki Nagel-Yüksel, Elena M. Willner, Jonas J. Funke, Thomas Gerling, Pierre Stömmer, Maximilian N. Honemann, Thomas G. Martin, Sjors H. W. Scheres, Hendrik Dietz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/fef625b2e3904bd18533db01a9a93d13
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spelling	oai:doaj.org-article:fef625b2e3904bd18533db01a9a93d132021-12-02T16:49:43ZRevealing the structures of megadalton-scale DNA complexes with nucleotide resolution10.1038/s41467-020-20020-72041-1723https://doaj.org/article/fef625b2e3904bd18533db01a9a93d132020-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20020-7https://doaj.org/toc/2041-1723Precision design of DNA origami needs precision validation. Here, the authors developed cryo-EM methods for obtaining high resolution structural data and for constructing pseudo-atomic models in a semi-automated fashion, allowing for iterative nanodevice inspection and refinement.Massimo KubeFabian KohlerElija FeiglBaki Nagel-YükselElena M. WillnerJonas J. FunkeThomas GerlingPierre StömmerMaximilian N. HonemannThomas G. MartinSjors H. W. ScheresHendrik DietzNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Massimo Kube Fabian Kohler Elija Feigl Baki Nagel-Yüksel Elena M. Willner Jonas J. Funke Thomas Gerling Pierre Stömmer Maximilian N. Honemann Thomas G. Martin Sjors H. W. Scheres Hendrik Dietz Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution
description	Precision design of DNA origami needs precision validation. Here, the authors developed cryo-EM methods for obtaining high resolution structural data and for constructing pseudo-atomic models in a semi-automated fashion, allowing for iterative nanodevice inspection and refinement.
format	article
author	Massimo Kube Fabian Kohler Elija Feigl Baki Nagel-Yüksel Elena M. Willner Jonas J. Funke Thomas Gerling Pierre Stömmer Maximilian N. Honemann Thomas G. Martin Sjors H. W. Scheres Hendrik Dietz
author_facet	Massimo Kube Fabian Kohler Elija Feigl Baki Nagel-Yüksel Elena M. Willner Jonas J. Funke Thomas Gerling Pierre Stömmer Maximilian N. Honemann Thomas G. Martin Sjors H. W. Scheres Hendrik Dietz
author_sort	Massimo Kube
title	Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution
title_short	Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution
title_full	Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution
title_fullStr	Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution
title_full_unstemmed	Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution
title_sort	revealing the structures of megadalton-scale dna complexes with nucleotide resolution
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/fef625b2e3904bd18533db01a9a93d13
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Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution

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