Bacterial diversity assessment in Antarctic terrestrial and aquatic microbial mats: a comparison between bidirectional pyrosequencing and cultivation.
The application of high-throughput sequencing of the 16S rRNA gene has increased the size of microbial diversity datasets by several orders of magnitude, providing improved access to the rare biosphere compared with cultivation-based approaches and more established cultivation-independent techniques...
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Main Authors: | Bjorn Tytgat, Elie Verleyen, Dagmar Obbels, Karolien Peeters, Aaike De Wever, Sofie D'hondt, Tim De Meyer, Wim Van Criekinge, Wim Vyverman, Anne Willems |
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Format: | article |
Language: | EN |
Published: |
Public Library of Science (PLoS)
2014
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Online Access: | https://doaj.org/article/ff535c37accc48a2a8b57174bbcfe4e4 |
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