Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria

Abstract Gold nanoparticles (AuNPs) aggregation-based colorimetric biosensing remains a challenge for bacteria due to their large size. Here we propose a novel colorimetric biosensor for rapid detection of Escherichia coli O157:H7 (E. coli O157:H7) in milk samples based on pH-regulated transformatio...

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Autores principales:	Xiahong Xu, Yuwei Yuan, Guixian Hu, Xiangyun Wang, Peipei Qi, Zhiwei Wang, Qiang Wang, Xinquan Wang, Yingchun Fu, Yanbin Li, Hua Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ff78ececcce64d79af56d88856f750da
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spelling	oai:doaj.org-article:ff78ececcce64d79af56d88856f750da2021-12-02T16:06:57ZExploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria10.1038/s41598-017-01371-62045-2322https://doaj.org/article/ff78ececcce64d79af56d88856f750da2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01371-6https://doaj.org/toc/2045-2322Abstract Gold nanoparticles (AuNPs) aggregation-based colorimetric biosensing remains a challenge for bacteria due to their large size. Here we propose a novel colorimetric biosensor for rapid detection of Escherichia coli O157:H7 (E. coli O157:H7) in milk samples based on pH-regulated transformation of dimer/tetramer of Concanavalin A (Con A) and the Con A-glycosyl recognition. Briefly, antibody-modified magnetic nanoparticles was used to capture and concentrate E. coli O157:H7 and then to label with Con A; pH adjusted to 5 was then applied to dissociate Con A tetramer to release dimer, which was collected and re-formed tetramer at pH of 7 to cause the aggregation of dextran-modified AuNPs. The interesting pH-dependent conformation-transformation behavior of Con A innovated the design of the release from the bacteria surface and then the reconstruction of Con A. Therefore, we realized the sensitive colorimetric biosensing of bacteria, which are much larger than AuNPs that is generally not suitable for this kind of method. The proposed biosensor exhibited a limit of detection down to 41 CFU/mL, short assay time (~95 min) and satisfactory specificity. The biosensor also worked well for the detection in milk sample, and may provide a universal concept for the design of colorimetric biosensors for bacteria and virus.Xiahong XuYuwei YuanGuixian HuXiangyun WangPeipei QiZhiwei WangQiang WangXinquan WangYingchun FuYanbin LiHua YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiahong Xu Yuwei Yuan Guixian Hu Xiangyun Wang Peipei Qi Zhiwei Wang Qiang Wang Xinquan Wang Yingchun Fu Yanbin Li Hua Yang Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria
description	Abstract Gold nanoparticles (AuNPs) aggregation-based colorimetric biosensing remains a challenge for bacteria due to their large size. Here we propose a novel colorimetric biosensor for rapid detection of Escherichia coli O157:H7 (E. coli O157:H7) in milk samples based on pH-regulated transformation of dimer/tetramer of Concanavalin A (Con A) and the Con A-glycosyl recognition. Briefly, antibody-modified magnetic nanoparticles was used to capture and concentrate E. coli O157:H7 and then to label with Con A; pH adjusted to 5 was then applied to dissociate Con A tetramer to release dimer, which was collected and re-formed tetramer at pH of 7 to cause the aggregation of dextran-modified AuNPs. The interesting pH-dependent conformation-transformation behavior of Con A innovated the design of the release from the bacteria surface and then the reconstruction of Con A. Therefore, we realized the sensitive colorimetric biosensing of bacteria, which are much larger than AuNPs that is generally not suitable for this kind of method. The proposed biosensor exhibited a limit of detection down to 41 CFU/mL, short assay time (~95 min) and satisfactory specificity. The biosensor also worked well for the detection in milk sample, and may provide a universal concept for the design of colorimetric biosensors for bacteria and virus.
format	article
author	Xiahong Xu Yuwei Yuan Guixian Hu Xiangyun Wang Peipei Qi Zhiwei Wang Qiang Wang Xinquan Wang Yingchun Fu Yanbin Li Hua Yang
author_facet	Xiahong Xu Yuwei Yuan Guixian Hu Xiangyun Wang Peipei Qi Zhiwei Wang Qiang Wang Xinquan Wang Yingchun Fu Yanbin Li Hua Yang
author_sort	Xiahong Xu
title	Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria
title_short	Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria
title_full	Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria
title_fullStr	Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria
title_full_unstemmed	Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria
title_sort	exploiting ph-regulated dimer-tetramer transformation of concanavalin a to develop colorimetric biosensing of bacteria
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ff78ececcce64d79af56d88856f750da
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_version_	1718384818835685376

Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria

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