ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms

Most human protein-coding genes are expressed as multiple isoforms. Here the authors present ORF Capture-seq that uses cloned ORFs as probes to capture and sequence full length transcript sequences. This enables highly sensitive characterization of eukaryotic transcriptomes.

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Auteurs principaux:	Gloria M. Sheynkman, Katharine S. Tuttle, Florent Laval, Elizabeth Tseng, Jason G. Underwood, Liang Yu, Da Dong, Melissa L. Smith, Robert Sebra, Luc Willems, Tong Hao, Michael A. Calderwood, David E. Hill, Marc Vidal
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2020
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/ffc16f1b155945bdb289f197b519b95e
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spelling	oai:doaj.org-article:ffc16f1b155945bdb289f197b519b95e2021-12-02T17:01:35ZORF Capture-Seq as a versatile method for targeted identification of full-length isoforms10.1038/s41467-020-16174-z2041-1723https://doaj.org/article/ffc16f1b155945bdb289f197b519b95e2020-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16174-zhttps://doaj.org/toc/2041-1723Most human protein-coding genes are expressed as multiple isoforms. Here the authors present ORF Capture-seq that uses cloned ORFs as probes to capture and sequence full length transcript sequences. This enables highly sensitive characterization of eukaryotic transcriptomes.Gloria M. SheynkmanKatharine S. TuttleFlorent LavalElizabeth TsengJason G. UnderwoodLiang YuDa DongMelissa L. SmithRobert SebraLuc WillemsTong HaoMichael A. CalderwoodDavid E. HillMarc VidalNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Gloria M. Sheynkman Katharine S. Tuttle Florent Laval Elizabeth Tseng Jason G. Underwood Liang Yu Da Dong Melissa L. Smith Robert Sebra Luc Willems Tong Hao Michael A. Calderwood David E. Hill Marc Vidal ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms
description	Most human protein-coding genes are expressed as multiple isoforms. Here the authors present ORF Capture-seq that uses cloned ORFs as probes to capture and sequence full length transcript sequences. This enables highly sensitive characterization of eukaryotic transcriptomes.
format	article
author	Gloria M. Sheynkman Katharine S. Tuttle Florent Laval Elizabeth Tseng Jason G. Underwood Liang Yu Da Dong Melissa L. Smith Robert Sebra Luc Willems Tong Hao Michael A. Calderwood David E. Hill Marc Vidal
author_facet	Gloria M. Sheynkman Katharine S. Tuttle Florent Laval Elizabeth Tseng Jason G. Underwood Liang Yu Da Dong Melissa L. Smith Robert Sebra Luc Willems Tong Hao Michael A. Calderwood David E. Hill Marc Vidal
author_sort	Gloria M. Sheynkman
title	ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms
title_short	ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms
title_full	ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms
title_fullStr	ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms
title_full_unstemmed	ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms
title_sort	orf capture-seq as a versatile method for targeted identification of full-length isoforms
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/ffc16f1b155945bdb289f197b519b95e
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ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms

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