ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms
Most human protein-coding genes are expressed as multiple isoforms. Here the authors present ORF Capture-seq that uses cloned ORFs as probes to capture and sequence full length transcript sequences. This enables highly sensitive characterization of eukaryotic transcriptomes.
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Nature Portfolio
2020
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oai:doaj.org-article:ffc16f1b155945bdb289f197b519b95e2021-12-02T17:01:35ZORF Capture-Seq as a versatile method for targeted identification of full-length isoforms10.1038/s41467-020-16174-z2041-1723https://doaj.org/article/ffc16f1b155945bdb289f197b519b95e2020-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16174-zhttps://doaj.org/toc/2041-1723Most human protein-coding genes are expressed as multiple isoforms. Here the authors present ORF Capture-seq that uses cloned ORFs as probes to capture and sequence full length transcript sequences. This enables highly sensitive characterization of eukaryotic transcriptomes.Gloria M. SheynkmanKatharine S. TuttleFlorent LavalElizabeth TsengJason G. UnderwoodLiang YuDa DongMelissa L. SmithRobert SebraLuc WillemsTong HaoMichael A. CalderwoodDavid E. HillMarc VidalNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020) |
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Science Q Gloria M. Sheynkman Katharine S. Tuttle Florent Laval Elizabeth Tseng Jason G. Underwood Liang Yu Da Dong Melissa L. Smith Robert Sebra Luc Willems Tong Hao Michael A. Calderwood David E. Hill Marc Vidal ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms |
description |
Most human protein-coding genes are expressed as multiple isoforms. Here the authors present ORF Capture-seq that uses cloned ORFs as probes to capture and sequence full length transcript sequences. This enables highly sensitive characterization of eukaryotic transcriptomes. |
format |
article |
author |
Gloria M. Sheynkman Katharine S. Tuttle Florent Laval Elizabeth Tseng Jason G. Underwood Liang Yu Da Dong Melissa L. Smith Robert Sebra Luc Willems Tong Hao Michael A. Calderwood David E. Hill Marc Vidal |
author_facet |
Gloria M. Sheynkman Katharine S. Tuttle Florent Laval Elizabeth Tseng Jason G. Underwood Liang Yu Da Dong Melissa L. Smith Robert Sebra Luc Willems Tong Hao Michael A. Calderwood David E. Hill Marc Vidal |
author_sort |
Gloria M. Sheynkman |
title |
ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms |
title_short |
ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms |
title_full |
ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms |
title_fullStr |
ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms |
title_full_unstemmed |
ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms |
title_sort |
orf capture-seq as a versatile method for targeted identification of full-length isoforms |
publisher |
Nature Portfolio |
publishDate |
2020 |
url |
https://doaj.org/article/ffc16f1b155945bdb289f197b519b95e |
work_keys_str_mv |
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