ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms

ORF Capture-Seq as a versatile method for targeted identification of full-length isoforms

Most human protein-coding genes are expressed as multiple isoforms. Here the authors present ORF Capture-seq that uses cloned ORFs as probes to capture and sequence full length transcript sequences. This enables highly sensitive characterization of eukaryotic transcriptomes.

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Detalles Bibliográficos
Autores principales: Gloria M. Sheynkman, Katharine S. Tuttle, Florent Laval, Elizabeth Tseng, Jason G. Underwood, Liang Yu, Da Dong, Melissa L. Smith, Robert Sebra, Luc Willems, Tong Hao, Michael A. Calderwood, David E. Hill, Marc Vidal
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
Materias:
Science
Q
Acceso en línea:https://doaj.org/article/ffc16f1b155945bdb289f197b519b95e
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