Structural basis for the multi-activity factor Rad5 in replication stress tolerance

Rad5 is a hub connecting three replication stress tolerance pathways. Here, the authors present the 3.3 Å crystal structure of a N-terminal truncated K.lactis Rad5 construct that reveals the spatial arrangement of the HIRAN, Snf2 and RING domains and structure-guided in vitro and in vivo experiments...

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Autores principales:	Miaomiao Shen, Nalini Dhingra, Quan Wang, Chen Cheng, Songbiao Zhu, Xiaolin Tian, Jun Yu, Xiaoxin Gong, Xuzhichao Li, Hongwei Zhang, Xin Xu, Liting Zhai, Min Xie, Ying Gao, Haiteng Deng, Yongning He, Hengyao Niu, Xiaolan Zhao, Song Xiang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/fffc4fbf2bca4bedb24e1f3731229166
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spelling	oai:doaj.org-article:fffc4fbf2bca4bedb24e1f37312291662021-12-02T14:01:13ZStructural basis for the multi-activity factor Rad5 in replication stress tolerance10.1038/s41467-020-20538-w2041-1723https://doaj.org/article/fffc4fbf2bca4bedb24e1f37312291662021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20538-whttps://doaj.org/toc/2041-1723Rad5 is a hub connecting three replication stress tolerance pathways. Here, the authors present the 3.3 Å crystal structure of a N-terminal truncated K.lactis Rad5 construct that reveals the spatial arrangement of the HIRAN, Snf2 and RING domains and structure-guided in vitro and in vivo experiments reveal multiple activities of the yeast Rad5 HIRAN domain among them a role in binding PCNA and supporting its ubiquitination.Miaomiao ShenNalini DhingraQuan WangChen ChengSongbiao ZhuXiaolin TianJun YuXiaoxin GongXuzhichao LiHongwei ZhangXin XuLiting ZhaiMin XieYing GaoHaiteng DengYongning HeHengyao NiuXiaolan ZhaoSong XiangNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Miaomiao Shen Nalini Dhingra Quan Wang Chen Cheng Songbiao Zhu Xiaolin Tian Jun Yu Xiaoxin Gong Xuzhichao Li Hongwei Zhang Xin Xu Liting Zhai Min Xie Ying Gao Haiteng Deng Yongning He Hengyao Niu Xiaolan Zhao Song Xiang Structural basis for the multi-activity factor Rad5 in replication stress tolerance
description	Rad5 is a hub connecting three replication stress tolerance pathways. Here, the authors present the 3.3 Å crystal structure of a N-terminal truncated K.lactis Rad5 construct that reveals the spatial arrangement of the HIRAN, Snf2 and RING domains and structure-guided in vitro and in vivo experiments reveal multiple activities of the yeast Rad5 HIRAN domain among them a role in binding PCNA and supporting its ubiquitination.
format	article
author	Miaomiao Shen Nalini Dhingra Quan Wang Chen Cheng Songbiao Zhu Xiaolin Tian Jun Yu Xiaoxin Gong Xuzhichao Li Hongwei Zhang Xin Xu Liting Zhai Min Xie Ying Gao Haiteng Deng Yongning He Hengyao Niu Xiaolan Zhao Song Xiang
author_facet	Miaomiao Shen Nalini Dhingra Quan Wang Chen Cheng Songbiao Zhu Xiaolin Tian Jun Yu Xiaoxin Gong Xuzhichao Li Hongwei Zhang Xin Xu Liting Zhai Min Xie Ying Gao Haiteng Deng Yongning He Hengyao Niu Xiaolan Zhao Song Xiang
author_sort	Miaomiao Shen
title	Structural basis for the multi-activity factor Rad5 in replication stress tolerance
title_short	Structural basis for the multi-activity factor Rad5 in replication stress tolerance
title_full	Structural basis for the multi-activity factor Rad5 in replication stress tolerance
title_fullStr	Structural basis for the multi-activity factor Rad5 in replication stress tolerance
title_full_unstemmed	Structural basis for the multi-activity factor Rad5 in replication stress tolerance
title_sort	structural basis for the multi-activity factor rad5 in replication stress tolerance
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fffc4fbf2bca4bedb24e1f3731229166
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Structural basis for the multi-activity factor Rad5 in replication stress tolerance

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