Polimorfismo +49 A/G del gen del antígeno 4 del linfocito T citotóxico (CTLA-4) en la diabetes tipo 1: Asociación con el perfil de anticuerpos y citoquinas

Background: Cytotoxic T lymphocyte associated antigen 4 (CTLA-4) has been one ofthe non HLA genes more commonly studied in type 1 diabetes mellitus (TID). CTLA-4 is a co-stimulation protein that has a key role in the negative regulation ofT cells and is related with a functional cytokine imbalance,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pérez B,Francisco, Codner D,Ethel, Ángel B,Bárbara, Balic N,Iván, Carrasco P,Elena
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2009
Materias:	CTLA-4 antigen Cytokines Diabetes Mellitus type 1
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000300001
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0034-98872009000300001
record_format	dspace
spelling	oai:scielo:S0034-988720090003000012009-06-15Polimorfismo +49 A/G del gen del antígeno 4 del linfocito T citotóxico (CTLA-4) en la diabetes tipo 1: Asociación con el perfil de anticuerpos y citoquinasPérez B,FranciscoCodner D,EthelÁngel B,BárbaraBalic N,IvánCarrasco P,Elena CTLA-4 antigen Cytokines Diabetes Mellitus type 1 Background: Cytotoxic T lymphocyte associated antigen 4 (CTLA-4) has been one ofthe non HLA genes more commonly studied in type 1 diabetes mellitus (TID). CTLA-4 is a co-stimulation protein that has a key role in the negative regulation ofT cells and is related with a functional cytokine imbalance, generating a T helper (Th) 1 over Th2 dominance. Aim: To analyze the association of +49 A/G polymorphism of CTLA-4 and its relationship with autoantibodies and cytokine expression in recently diagnosed TID patients. Patients and Methods: CTLA-4 genetic variants and auto-antibody levéis were studied in 260 chiídren with TID and 255 healthy chiídren matched by age and gender +49 A/G polymorphism of CTLA-4 was studied by polymerase chain reaction and restriction fragmentpolymorphism (PCR-RFLP). Autoantibody levéis were measured by conventional ELISA. A panel of60 cytokines was studied simultaneously by serum array analysis in 15 TID and 15 healthy controls stratified according CTLA-4 genotype. Results: The +49 A/G genetic frequency was similar in TID cases and healthy chiídren. A positive anti-GAD65 and anti-IA-2 level was observed in 673% of TID group. This percentage was increased among GG carriers (79.4% to GAD65 and 70.6% to IA-2). Finally, TID patients carrying this genotype showed a high expression of interleukin 2, 10, tumor necrosis factor alpha and interferon gamma. Conclusions: The +49 A/G polymorphism of CTLA-4 was similar in diabetic and control chiídren. Among patients with TID and carriers of GG genotype, a higher frequency of anti-GAD65 and a preferential Thl cytokine expression profile was observed.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.137 n.3 20092009-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000300001es10.4067/S0034-98872009000300001
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	CTLA-4 antigen Cytokines Diabetes Mellitus type 1
spellingShingle	CTLA-4 antigen Cytokines Diabetes Mellitus type 1 Pérez B,Francisco Codner D,Ethel Ángel B,Bárbara Balic N,Iván Carrasco P,Elena Polimorfismo +49 A/G del gen del antígeno 4 del linfocito T citotóxico (CTLA-4) en la diabetes tipo 1: Asociación con el perfil de anticuerpos y citoquinas
description	Background: Cytotoxic T lymphocyte associated antigen 4 (CTLA-4) has been one ofthe non HLA genes more commonly studied in type 1 diabetes mellitus (TID). CTLA-4 is a co-stimulation protein that has a key role in the negative regulation ofT cells and is related with a functional cytokine imbalance, generating a T helper (Th) 1 over Th2 dominance. Aim: To analyze the association of +49 A/G polymorphism of CTLA-4 and its relationship with autoantibodies and cytokine expression in recently diagnosed TID patients. Patients and Methods: CTLA-4 genetic variants and auto-antibody levéis were studied in 260 chiídren with TID and 255 healthy chiídren matched by age and gender +49 A/G polymorphism of CTLA-4 was studied by polymerase chain reaction and restriction fragmentpolymorphism (PCR-RFLP). Autoantibody levéis were measured by conventional ELISA. A panel of60 cytokines was studied simultaneously by serum array analysis in 15 TID and 15 healthy controls stratified according CTLA-4 genotype. Results: The +49 A/G genetic frequency was similar in TID cases and healthy chiídren. A positive anti-GAD65 and anti-IA-2 level was observed in 673% of TID group. This percentage was increased among GG carriers (79.4% to GAD65 and 70.6% to IA-2). Finally, TID patients carrying this genotype showed a high expression of interleukin 2, 10, tumor necrosis factor alpha and interferon gamma. Conclusions: The +49 A/G polymorphism of CTLA-4 was similar in diabetic and control chiídren. Among patients with TID and carriers of GG genotype, a higher frequency of anti-GAD65 and a preferential Thl cytokine expression profile was observed.
author	Pérez B,Francisco Codner D,Ethel Ángel B,Bárbara Balic N,Iván Carrasco P,Elena
author_facet	Pérez B,Francisco Codner D,Ethel Ángel B,Bárbara Balic N,Iván Carrasco P,Elena
author_sort	Pérez B,Francisco
title	Polimorfismo +49 A/G del gen del antígeno 4 del linfocito T citotóxico (CTLA-4) en la diabetes tipo 1: Asociación con el perfil de anticuerpos y citoquinas
title_short	Polimorfismo +49 A/G del gen del antígeno 4 del linfocito T citotóxico (CTLA-4) en la diabetes tipo 1: Asociación con el perfil de anticuerpos y citoquinas
title_full	Polimorfismo +49 A/G del gen del antígeno 4 del linfocito T citotóxico (CTLA-4) en la diabetes tipo 1: Asociación con el perfil de anticuerpos y citoquinas
title_fullStr	Polimorfismo +49 A/G del gen del antígeno 4 del linfocito T citotóxico (CTLA-4) en la diabetes tipo 1: Asociación con el perfil de anticuerpos y citoquinas
title_full_unstemmed	Polimorfismo +49 A/G del gen del antígeno 4 del linfocito T citotóxico (CTLA-4) en la diabetes tipo 1: Asociación con el perfil de anticuerpos y citoquinas
title_sort	polimorfismo +49 a/g del gen del antígeno 4 del linfocito t citotóxico (ctla-4) en la diabetes tipo 1: asociación con el perfil de anticuerpos y citoquinas
publisher	Sociedad Médica de Santiago
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000300001
work_keys_str_mv	AT perezbfrancisco polimorfismo49agdelgendelantigeno4dellinfocitotcitotoxicoctla4enladiabetestipo1asociacionconelperfildeanticuerposycitoquinas AT codnerdethel polimorfismo49agdelgendelantigeno4dellinfocitotcitotoxicoctla4enladiabetestipo1asociacionconelperfildeanticuerposycitoquinas AT angelbbarbara polimorfismo49agdelgendelantigeno4dellinfocitotcitotoxicoctla4enladiabetestipo1asociacionconelperfildeanticuerposycitoquinas AT balicnivan polimorfismo49agdelgendelantigeno4dellinfocitotcitotoxicoctla4enladiabetestipo1asociacionconelperfildeanticuerposycitoquinas AT carrascopelena polimorfismo49agdelgendelantigeno4dellinfocitotcitotoxicoctla4enladiabetestipo1asociacionconelperfildeanticuerposycitoquinas
_version_	1718436430556954624

Polimorfismo +49 A/G del gen del antígeno 4 del linfocito T citotóxico (CTLA-4) en la diabetes tipo 1: Asociación con el perfil de anticuerpos y citoquinas

Ejemplares similares