The cellular mechanisms of body iron homeostasis

Cells tightly regulate iron levels through the activity of iron regulatory proteins (IRPs) that bind to RNA motifs called iron responsive elements (IREs). When cells become iron-depleted, IRPs bind to IREs present in the mRNAs of ferritin and the transferrin receptor, resulting in diminished transla...

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Autores principales:	NUÑEZ,MARCO T, GARATE,MARCO A, ARREDONDO,MIGUEL, TAPlA,VICTORIA, MUÑOZ,PATRICIA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2000
Materias:	metal ions intestinal absorption ferritin transferrin homeostasis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200013
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spelling	oai:scielo:S0716-976020000002000132010-02-11The cellular mechanisms of body iron homeostasisNUÑEZ,MARCO TGARATE,MARCO AARREDONDO,MIGUELTAPlA,VICTORIAMUÑOZ,PATRICIA metal ions intestinal absorption ferritin transferrin homeostasis Cells tightly regulate iron levels through the activity of iron regulatory proteins (IRPs) that bind to RNA motifs called iron responsive elements (IREs). When cells become iron-depleted, IRPs bind to IREs present in the mRNAs of ferritin and the transferrin receptor, resulting in diminished translation of the ferritin mRNA and increased translation of the transferrin receptor mRNA. Similarly, body iron homeostasis is maintained through the control of intestinal iron absorption. Intestinal epithelia cells sense body iron through the basolateral endocytosis of plasma transferrin. Transterrin endocytosis results in enterocytes whose iron content will depend on the iron saturation of plasma transferrin. Cell iron levels, in turn, inversely correlate with intestinal iron absorption. In this study, we examined the relationship between the regulation of intestinal iron absorption and the regulation of intracellular iron levels by Caco-2 cells. We asserted that IRP activity closely correlates with apical iron uptake and transepithelial iron transport. Moreover, overexpression of IRE resulted in a very low labile or reactive iron pool and increased apical to basolateral iron flux. These results show that iron absorption is primarily regulated by the size of the labile iron pool, which in turn is regulated by the IRE/IRP system.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.33 n.2 20002000-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200013en10.4067/S0716-97602000000200013
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	metal ions intestinal absorption ferritin transferrin homeostasis
spellingShingle	metal ions intestinal absorption ferritin transferrin homeostasis NUÑEZ,MARCO T GARATE,MARCO A ARREDONDO,MIGUEL TAPlA,VICTORIA MUÑOZ,PATRICIA The cellular mechanisms of body iron homeostasis
description	Cells tightly regulate iron levels through the activity of iron regulatory proteins (IRPs) that bind to RNA motifs called iron responsive elements (IREs). When cells become iron-depleted, IRPs bind to IREs present in the mRNAs of ferritin and the transferrin receptor, resulting in diminished translation of the ferritin mRNA and increased translation of the transferrin receptor mRNA. Similarly, body iron homeostasis is maintained through the control of intestinal iron absorption. Intestinal epithelia cells sense body iron through the basolateral endocytosis of plasma transferrin. Transterrin endocytosis results in enterocytes whose iron content will depend on the iron saturation of plasma transferrin. Cell iron levels, in turn, inversely correlate with intestinal iron absorption. In this study, we examined the relationship between the regulation of intestinal iron absorption and the regulation of intracellular iron levels by Caco-2 cells. We asserted that IRP activity closely correlates with apical iron uptake and transepithelial iron transport. Moreover, overexpression of IRE resulted in a very low labile or reactive iron pool and increased apical to basolateral iron flux. These results show that iron absorption is primarily regulated by the size of the labile iron pool, which in turn is regulated by the IRE/IRP system.
author	NUÑEZ,MARCO T GARATE,MARCO A ARREDONDO,MIGUEL TAPlA,VICTORIA MUÑOZ,PATRICIA
author_facet	NUÑEZ,MARCO T GARATE,MARCO A ARREDONDO,MIGUEL TAPlA,VICTORIA MUÑOZ,PATRICIA
author_sort	NUÑEZ,MARCO T
title	The cellular mechanisms of body iron homeostasis
title_short	The cellular mechanisms of body iron homeostasis
title_full	The cellular mechanisms of body iron homeostasis
title_fullStr	The cellular mechanisms of body iron homeostasis
title_full_unstemmed	The cellular mechanisms of body iron homeostasis
title_sort	cellular mechanisms of body iron homeostasis
publisher	Sociedad de Biología de Chile
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200013
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The cellular mechanisms of body iron homeostasis

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