Grape seed extract proanthocyanidins downregulate HIV- 1 entry coreceptors, CCR2b, CCR3 and CCR5 gene expression by normal peripheral blood mononuclear cells
Flavonoids and related polyphenols, in addition to their cardioprotective, anti-tumor, anti-inflammatory, anti-carcinogenic and anti-allergic activities, also possess promising anti-HIV effects. Recent studies documented that the ß-chemokine receptors, CCR2b, CCR3 and CCR5, and the alpha-chemokine r...
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Sociedad de Biología de Chile
2002
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oai:scielo:S0716-976020020003000162003-11-26Grape seed extract proanthocyanidins downregulate HIV- 1 entry coreceptors, CCR2b, CCR3 and CCR5 gene expression by normal peripheral blood mononuclear cellsNAIR,MADHAVAN PKANDASWAMI,CHITHANMAHAJAN,SUPRIYANAIR,HARIKRISHNA NCHAWDA,RAMSHANAHAN,THOMASSCHWARTZ,STANLEY A PBMC, peripheral blood mononuclear cells RT- PCR - reverse transcriptase polymerase chain reaction HIV-1 , human immunodeficiency virus-1 GSE,- Grape Seed Extract Flavonoids and related polyphenols, in addition to their cardioprotective, anti-tumor, anti-inflammatory, anti-carcinogenic and anti-allergic activities, also possess promising anti-HIV effects. Recent studies documented that the ß-chemokine receptors, CCR2b, CCR3 and CCR5, and the alpha-chemokine receptors, CXCR1, CXCR2 and CXCR4 serve as entry coreceptors for HIV-1. Although flavonoids and polyphenolic compounds elicit anti-HIV effects such as inhibition of HIV-1 expression and virus replication, the molecular mechanisms underlying these effects remain to be clearly elucidated. We hypothesize that flavonoids exert their anti-HIV effects, possibly by interfering at the HIV co-receptor level. We investigated the effect of flavonoid constituents of a proprietary grape seed extract (GSE) on the expression of HIV-1 coentry receptors by immunocompetent mononuclear leukocytes. Our results showed that GSE significantly downregulated the expression of the HIV-1 entry co-receptors, CCR2b , CCR3 and CCR5 in normal PBMC in a dose dependent manner. Further , GSE treated cultures showed significantly lower number of CCR3 positive cells as quantitated by flow cytometry analysis which supports RT-PCR gene expression data.Investigations of the mechanisms underlying the anti-HIV-1 effects of grape seed extracts may help to identify promising natural products useful in the prevention and /or amelioration of HIV-1 infectioninfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.35 n.3-4 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300016en10.4067/S0716-97602002000300016 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
PBMC, peripheral blood mononuclear cells RT- PCR - reverse transcriptase polymerase chain reaction HIV-1 , human immunodeficiency virus-1 GSE,- Grape Seed Extract |
| spellingShingle |
PBMC, peripheral blood mononuclear cells RT- PCR - reverse transcriptase polymerase chain reaction HIV-1 , human immunodeficiency virus-1 GSE,- Grape Seed Extract NAIR,MADHAVAN P KANDASWAMI,CHITHAN MAHAJAN,SUPRIYA NAIR,HARIKRISHNA N CHAWDA,RAM SHANAHAN,THOMAS SCHWARTZ,STANLEY A Grape seed extract proanthocyanidins downregulate HIV- 1 entry coreceptors, CCR2b, CCR3 and CCR5 gene expression by normal peripheral blood mononuclear cells |
| description |
Flavonoids and related polyphenols, in addition to their cardioprotective, anti-tumor, anti-inflammatory, anti-carcinogenic and anti-allergic activities, also possess promising anti-HIV effects. Recent studies documented that the ß-chemokine receptors, CCR2b, CCR3 and CCR5, and the alpha-chemokine receptors, CXCR1, CXCR2 and CXCR4 serve as entry coreceptors for HIV-1. Although flavonoids and polyphenolic compounds elicit anti-HIV effects such as inhibition of HIV-1 expression and virus replication, the molecular mechanisms underlying these effects remain to be clearly elucidated. We hypothesize that flavonoids exert their anti-HIV effects, possibly by interfering at the HIV co-receptor level. We investigated the effect of flavonoid constituents of a proprietary grape seed extract (GSE) on the expression of HIV-1 coentry receptors by immunocompetent mononuclear leukocytes. Our results showed that GSE significantly downregulated the expression of the HIV-1 entry co-receptors, CCR2b , CCR3 and CCR5 in normal PBMC in a dose dependent manner. Further , GSE treated cultures showed significantly lower number of CCR3 positive cells as quantitated by flow cytometry analysis which supports RT-PCR gene expression data.Investigations of the mechanisms underlying the anti-HIV-1 effects of grape seed extracts may help to identify promising natural products useful in the prevention and /or amelioration of HIV-1 infection |
| author |
NAIR,MADHAVAN P KANDASWAMI,CHITHAN MAHAJAN,SUPRIYA NAIR,HARIKRISHNA N CHAWDA,RAM SHANAHAN,THOMAS SCHWARTZ,STANLEY A |
| author_facet |
NAIR,MADHAVAN P KANDASWAMI,CHITHAN MAHAJAN,SUPRIYA NAIR,HARIKRISHNA N CHAWDA,RAM SHANAHAN,THOMAS SCHWARTZ,STANLEY A |
| author_sort |
NAIR,MADHAVAN P |
| title |
Grape seed extract proanthocyanidins downregulate HIV- 1 entry coreceptors, CCR2b, CCR3 and CCR5 gene expression by normal peripheral blood mononuclear cells |
| title_short |
Grape seed extract proanthocyanidins downregulate HIV- 1 entry coreceptors, CCR2b, CCR3 and CCR5 gene expression by normal peripheral blood mononuclear cells |
| title_full |
Grape seed extract proanthocyanidins downregulate HIV- 1 entry coreceptors, CCR2b, CCR3 and CCR5 gene expression by normal peripheral blood mononuclear cells |
| title_fullStr |
Grape seed extract proanthocyanidins downregulate HIV- 1 entry coreceptors, CCR2b, CCR3 and CCR5 gene expression by normal peripheral blood mononuclear cells |
| title_full_unstemmed |
Grape seed extract proanthocyanidins downregulate HIV- 1 entry coreceptors, CCR2b, CCR3 and CCR5 gene expression by normal peripheral blood mononuclear cells |
| title_sort |
grape seed extract proanthocyanidins downregulate hiv- 1 entry coreceptors, ccr2b, ccr3 and ccr5 gene expression by normal peripheral blood mononuclear cells |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2002 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300016 |
| work_keys_str_mv |
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