A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies

A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies

A novel procedure for the evaluation of total reactive antioxidant potentials (TRAP) is described. The method is based on the measurement of the bleaching of pyranine by peroxyl radicals. The addition of the antioxidants produces a clear induction time whose magnitude is directly related to the anti...

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Autores principales: CAMPOS,ANA MARÍA, SOTOMAYOR,CARLOS PATRICIO, PINO,EDUARDO, LISSI,EDUARDO
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2004
Materias:
Peroxyl radicals
polyphenols
pyranine
total reactive antioxidant potential
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200015
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spelling oai:scielo:S0716-976020040002000152005-01-06A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related MethodologiesCAMPOS,ANA MARÍASOTOMAYOR,CARLOS PATRICIOPINO,EDUARDOLISSI,EDUARDO Peroxyl radicals polyphenols pyranine total reactive antioxidant potential A novel procedure for the evaluation of total reactive antioxidant potentials (TRAP) is described. The method is based on the measurement of the bleaching of pyranine by peroxyl radicals. The addition of the antioxidants produces a clear induction time whose magnitude is directly related to the antioxidant concentration. A comparison of the values obtained with those reported employing closely related methodologies shows that the results are significantly affected by the substrate employed to monitor the steady state free radical concentration. Possible sources of this dependence are discussedinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.2 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200015en10.4067/S0716-97602004000200015
institution Scielo Chile
collection Scielo Chile
language English
topic Peroxyl radicals
polyphenols
pyranine
total reactive antioxidant potential
spellingShingle Peroxyl radicals
polyphenols
pyranine
total reactive antioxidant potential
CAMPOS,ANA MARÍA
SOTOMAYOR,CARLOS PATRICIO
PINO,EDUARDO
LISSI,EDUARDO
A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies
description A novel procedure for the evaluation of total reactive antioxidant potentials (TRAP) is described. The method is based on the measurement of the bleaching of pyranine by peroxyl radicals. The addition of the antioxidants produces a clear induction time whose magnitude is directly related to the antioxidant concentration. A comparison of the values obtained with those reported employing closely related methodologies shows that the results are significantly affected by the substrate employed to monitor the steady state free radical concentration. Possible sources of this dependence are discussed
author CAMPOS,ANA MARÍA
SOTOMAYOR,CARLOS PATRICIO
PINO,EDUARDO
LISSI,EDUARDO
author_facet CAMPOS,ANA MARÍA
SOTOMAYOR,CARLOS PATRICIO
PINO,EDUARDO
LISSI,EDUARDO
author_sort CAMPOS,ANA MARÍA
title A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies
title_short A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies
title_full A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies
title_fullStr A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies
title_full_unstemmed A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies
title_sort pyranine based procedure for evaluation of the total antioxidant potential (trap) of polyphenols: a comparison with closely related methodologies
publisher Sociedad de Biología de Chile
publishDate 2004
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200015
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AT pinoeduardo apyraninebasedprocedureforevaluationofthetotalantioxidantpotentialtrapofpolyphenolsacomparisonwithcloselyrelatedmethodologies
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