Effect of 0.25 ppm Ozone exposure on pulmonary damage induced by bleomycin

To study the effect of ozone in a chronically damaged lung, we used a bleomycin (BLM) induced pulmonary fibrosis model. Both endotracheal instillation of BLM and O3 exposure both produce lung inflammation and fibrosis. Oxidative stress would be a common mechanism of damage for both BLM and O3. Our a...

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Auteurs principaux:	OYARZÚN,MANUEL, DUSSAUBAT,NELSON, GONZÁLEZ,SERGIO
Langue:	English
Publié:	Sociedad de Biología de Chile 2005
Sujets:	bleomycin histopathology lung damage ozone exposure
Accès en ligne:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400007
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spelling	oai:scielo:S0716-976020050004000072006-04-18Effect of 0.25 ppm Ozone exposure on pulmonary damage induced by bleomycinOYARZÚN,MANUELDUSSAUBAT,NELSONGONZÁLEZ,SERGIO bleomycin histopathology lung damage ozone exposure To study the effect of ozone in a chronically damaged lung, we used a bleomycin (BLM) induced pulmonary fibrosis model. Both endotracheal instillation of BLM and O3 exposure both produce lung inflammation and fibrosis. Oxidative stress would be a common mechanism of damage for both BLM and O3. Our aim was to assess lung injury induced by 5 and 60 days of intermittent exposure to 0.25 ppm O3 in rats with bleomycin-induced pulmonary fibrosis. Thirty-day-old Sprague Dawley rats were endotracheally instilled with BLM (1 U/100 g body weight) and, 30 days later, exposed to 0.25 ppm O3 (0.25 ppm 4 h per day, 5 days a week). Histopatology controls were instilled with saline and breathing room air. Histopathological evaluation of lungs was done 5 and 60 days after O3 exposure. BLM-induced lung damage did not change after 60 days of intermittent O3 exposure. Five days of O3 exposure increased the mean score of BLM-induced pulmonary inflammation and fibrosis (p=0.06). Frequency of bronchopneumonia increased from 1/7 to 6/6 (p <0.001), suggesting that a short-term exposure to O3 in a previously damaged lung might be a risk factor for developing further lung injuryinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.38 n.4 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400007en10.4067/S0716-97602005000400007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bleomycin histopathology lung damage ozone exposure
spellingShingle	bleomycin histopathology lung damage ozone exposure OYARZÚN,MANUEL DUSSAUBAT,NELSON GONZÁLEZ,SERGIO Effect of 0.25 ppm Ozone exposure on pulmonary damage induced by bleomycin
description	To study the effect of ozone in a chronically damaged lung, we used a bleomycin (BLM) induced pulmonary fibrosis model. Both endotracheal instillation of BLM and O3 exposure both produce lung inflammation and fibrosis. Oxidative stress would be a common mechanism of damage for both BLM and O3. Our aim was to assess lung injury induced by 5 and 60 days of intermittent exposure to 0.25 ppm O3 in rats with bleomycin-induced pulmonary fibrosis. Thirty-day-old Sprague Dawley rats were endotracheally instilled with BLM (1 U/100 g body weight) and, 30 days later, exposed to 0.25 ppm O3 (0.25 ppm 4 h per day, 5 days a week). Histopatology controls were instilled with saline and breathing room air. Histopathological evaluation of lungs was done 5 and 60 days after O3 exposure. BLM-induced lung damage did not change after 60 days of intermittent O3 exposure. Five days of O3 exposure increased the mean score of BLM-induced pulmonary inflammation and fibrosis (p=0.06). Frequency of bronchopneumonia increased from 1/7 to 6/6 (p <0.001), suggesting that a short-term exposure to O3 in a previously damaged lung might be a risk factor for developing further lung injury
author	OYARZÚN,MANUEL DUSSAUBAT,NELSON GONZÁLEZ,SERGIO
author_facet	OYARZÚN,MANUEL DUSSAUBAT,NELSON GONZÁLEZ,SERGIO
author_sort	OYARZÚN,MANUEL
title	Effect of 0.25 ppm Ozone exposure on pulmonary damage induced by bleomycin
title_short	Effect of 0.25 ppm Ozone exposure on pulmonary damage induced by bleomycin
title_full	Effect of 0.25 ppm Ozone exposure on pulmonary damage induced by bleomycin
title_fullStr	Effect of 0.25 ppm Ozone exposure on pulmonary damage induced by bleomycin
title_full_unstemmed	Effect of 0.25 ppm Ozone exposure on pulmonary damage induced by bleomycin
title_sort	effect of 0.25 ppm ozone exposure on pulmonary damage induced by bleomycin
publisher	Sociedad de Biología de Chile
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400007
work_keys_str_mv	AT oyarzunmanuel effectof025ppmozoneexposureonpulmonarydamageinducedbybleomycin AT dussaubatnelson effectof025ppmozoneexposureonpulmonarydamageinducedbybleomycin AT gonzalezsergio effectof025ppmozoneexposureonpulmonarydamageinducedbybleomycin
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Effect of 0.25 ppm Ozone exposure on pulmonary damage induced by bleomycin

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