Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets

Thymeleatoxin (TMX), an activator of Ca2+-sensitive protein kinase C (cPKC) isoforms, was used to assess the PKC isoform specificity of cholinergic potentiation of glucose (11 mM)-induced pulsatile 5-HT/insulin release (PIR) from single mouse pancreatic islets. TMX (100 nM) and carbachol (Cch, 50 mM...

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Autores principales:	BALDEIRAS,INÊS E, SANTOS,ROSA M, ROSÁRIO,LUÍS M
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	Cytosolic free Ca2+ concentration 5-HT amperometry islet of Langerhans protein kinase C pulsatile insulin release thymeleatoxin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300014
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spelling	oai:scielo:S0716-976020060003000142014-01-24Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic isletsBALDEIRAS,INÊS ESANTOS,ROSA MROSÁRIO,LUÍS M Cytosolic free Ca2+ concentration 5-HT amperometry islet of Langerhans protein kinase C pulsatile insulin release thymeleatoxin Thymeleatoxin (TMX), an activator of Ca2+-sensitive protein kinase C (cPKC) isoforms, was used to assess the PKC isoform specificity of cholinergic potentiation of glucose (11 mM)-induced pulsatile 5-HT/insulin release (PIR) from single mouse pancreatic islets. TMX (100 nM) and carbachol (Cch, 50 mM) enhanced PIR ~ 3-fold while reducing the underlying [Ca2+]i oscillations (duration and amplitude) by ~ 40-50%. Both effects were ablated by the specific PKC inhibitor bisindolylmaleimide and chronic TMX pretreatment. Cch also evoked an initial transient [Ca2+]i rise and surge of 5-HT release, which remained unaffected by chronic TMX pretreatment. It is concluded that the immediate cholinergic responses are insensitive to cPKC. In contrast, specific activation of a cPKC isoform mediates sustained cholinergic potentiation of glucose-induced insulin secretion.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.3 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300014en10.4067/S0716-97602006000300014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cytosolic free Ca2+ concentration 5-HT amperometry islet of Langerhans protein kinase C pulsatile insulin release thymeleatoxin
spellingShingle	Cytosolic free Ca2+ concentration 5-HT amperometry islet of Langerhans protein kinase C pulsatile insulin release thymeleatoxin BALDEIRAS,INÊS E SANTOS,ROSA M ROSÁRIO,LUÍS M Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets
description	Thymeleatoxin (TMX), an activator of Ca2+-sensitive protein kinase C (cPKC) isoforms, was used to assess the PKC isoform specificity of cholinergic potentiation of glucose (11 mM)-induced pulsatile 5-HT/insulin release (PIR) from single mouse pancreatic islets. TMX (100 nM) and carbachol (Cch, 50 mM) enhanced PIR ~ 3-fold while reducing the underlying [Ca2+]i oscillations (duration and amplitude) by ~ 40-50%. Both effects were ablated by the specific PKC inhibitor bisindolylmaleimide and chronic TMX pretreatment. Cch also evoked an initial transient [Ca2+]i rise and surge of 5-HT release, which remained unaffected by chronic TMX pretreatment. It is concluded that the immediate cholinergic responses are insensitive to cPKC. In contrast, specific activation of a cPKC isoform mediates sustained cholinergic potentiation of glucose-induced insulin secretion.
author	BALDEIRAS,INÊS E SANTOS,ROSA M ROSÁRIO,LUÍS M
author_facet	BALDEIRAS,INÊS E SANTOS,ROSA M ROSÁRIO,LUÍS M
author_sort	BALDEIRAS,INÊS E
title	Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets
title_short	Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets
title_full	Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets
title_fullStr	Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets
title_full_unstemmed	Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets
title_sort	protein kinase c isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-ht/ insulin release from mouse pancreatic islets
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300014
work_keys_str_mv	AT baldeirasinese proteinkinasecisoformspecificityofcholinergicpotentiationofglucoseinducedpulsatile5htinsulinreleasefrommousepancreaticislets AT santosrosam proteinkinasecisoformspecificityofcholinergicpotentiationofglucoseinducedpulsatile5htinsulinreleasefrommousepancreaticislets AT rosarioluism proteinkinasecisoformspecificityofcholinergicpotentiationofglucoseinducedpulsatile5htinsulinreleasefrommousepancreaticislets
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Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets

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