Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant

In the present work, immunogenicity of recombinant in vitro assembled hepatitis C virus core particles, HCcAg.120-VLPs, either alone or in combination with different adjuvants was evaluated in BALB/c mice. HCcAg.120-VLPs induced high titers of anti-HCcAg.120 antibodies and virus-specific cellular im...

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Autores principales:	ACOSTA-RIVERO,NELSON, POUTOU,JOANNA, ÁLVAREZ-LAJONCHERE,LIZ, GUERRA,IVIS, AGUILERA,YARAIMA, MUSACCHIO,ALEXIS, RODRÍGUEZ,ARMANDO, AGUILAR,JULIO C, FALCON,VIVIANA, ÁLVAREZ-OBREGON,JULIO C, SORIA,YORDANKA, TORRES,DINORAH, LINARES,MARBELIS, PÉREZ,ÁNGEL, MORALES-GRILLO,JUAN, DUEÑAS -CARRERA,SANTIAGO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	challenge HCV immunity adjuvant capsid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100005
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spelling	oai:scielo:S0716-976020090001000052009-06-11Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvantACOSTA-RIVERO,NELSONPOUTOU,JOANNAÁLVAREZ-LAJONCHERE,LIZGUERRA,IVISAGUILERA,YARAIMAMUSACCHIO,ALEXISRODRÍGUEZ,ARMANDOAGUILAR,JULIO CFALCON,VIVIANAÁLVAREZ-OBREGON,JULIO CSORIA,YORDANKATORRES,DINORAHLINARES,MARBELISPÉREZ,ÁNGELMORALES-GRILLO,JUANDUEÑAS -CARRERA,SANTIAGO challenge HCV immunity adjuvant capsid In the present work, immunogenicity of recombinant in vitro assembled hepatitis C virus core particles, HCcAg.120-VLPs, either alone or in combination with different adjuvants was evaluated in BALB/c mice. HCcAg.120-VLPs induced high titers of anti-HCcAg.120 antibodies and virus-specific cellular immune responses. Particularly, HCcAg.120-VLPs induced specific delayed type hypersensitivity, and generated a predominant T helper 1 cytokine pro file in immunized mice. In addition, HCcAg.120-VLPs prime splenocytes proliferate in vitro against different HCcAg.120-specific peptides, depending on either the immunization route or the adjuvant used. Remarkably, immunization with HCcAg.120-VLPs/Montanide ISA888 formulation resulted in a significant control of vaccinia virus titer in mice after challenge with a recombinant vaccinia virus expressing HCV core protein, vvCore. Animals immunized with this formulation had a marked increase in the number of IFN-γ producing spleen cells, after stimulation with P815 cells infected with vvCore. These results suggest the use of recombinant HCV core particles as components of therapeutic or preventive vaccine candidates against HCV.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.1 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100005en10.4067/S0716-97602009000100005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	challenge HCV immunity adjuvant capsid
spellingShingle	challenge HCV immunity adjuvant capsid ACOSTA-RIVERO,NELSON POUTOU,JOANNA ÁLVAREZ-LAJONCHERE,LIZ GUERRA,IVIS AGUILERA,YARAIMA MUSACCHIO,ALEXIS RODRÍGUEZ,ARMANDO AGUILAR,JULIO C FALCON,VIVIANA ÁLVAREZ-OBREGON,JULIO C SORIA,YORDANKA TORRES,DINORAH LINARES,MARBELIS PÉREZ,ÁNGEL MORALES-GRILLO,JUAN DUEÑAS -CARRERA,SANTIAGO Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant
description	In the present work, immunogenicity of recombinant in vitro assembled hepatitis C virus core particles, HCcAg.120-VLPs, either alone or in combination with different adjuvants was evaluated in BALB/c mice. HCcAg.120-VLPs induced high titers of anti-HCcAg.120 antibodies and virus-specific cellular immune responses. Particularly, HCcAg.120-VLPs induced specific delayed type hypersensitivity, and generated a predominant T helper 1 cytokine pro file in immunized mice. In addition, HCcAg.120-VLPs prime splenocytes proliferate in vitro against different HCcAg.120-specific peptides, depending on either the immunization route or the adjuvant used. Remarkably, immunization with HCcAg.120-VLPs/Montanide ISA888 formulation resulted in a significant control of vaccinia virus titer in mice after challenge with a recombinant vaccinia virus expressing HCV core protein, vvCore. Animals immunized with this formulation had a marked increase in the number of IFN-γ producing spleen cells, after stimulation with P815 cells infected with vvCore. These results suggest the use of recombinant HCV core particles as components of therapeutic or preventive vaccine candidates against HCV.
author	ACOSTA-RIVERO,NELSON POUTOU,JOANNA ÁLVAREZ-LAJONCHERE,LIZ GUERRA,IVIS AGUILERA,YARAIMA MUSACCHIO,ALEXIS RODRÍGUEZ,ARMANDO AGUILAR,JULIO C FALCON,VIVIANA ÁLVAREZ-OBREGON,JULIO C SORIA,YORDANKA TORRES,DINORAH LINARES,MARBELIS PÉREZ,ÁNGEL MORALES-GRILLO,JUAN DUEÑAS -CARRERA,SANTIAGO
author_facet	ACOSTA-RIVERO,NELSON POUTOU,JOANNA ÁLVAREZ-LAJONCHERE,LIZ GUERRA,IVIS AGUILERA,YARAIMA MUSACCHIO,ALEXIS RODRÍGUEZ,ARMANDO AGUILAR,JULIO C FALCON,VIVIANA ÁLVAREZ-OBREGON,JULIO C SORIA,YORDANKA TORRES,DINORAH LINARES,MARBELIS PÉREZ,ÁNGEL MORALES-GRILLO,JUAN DUEÑAS -CARRERA,SANTIAGO
author_sort	ACOSTA-RIVERO,NELSON
title	Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant
title_short	Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant
title_full	Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant
title_fullStr	Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant
title_full_unstemmed	Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant
title_sort	recombinant in vitro assembled hepatitis c virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100005
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Recombinant in vitro assembled hepatitis C virus core particles induce strong specific immunity enhanced by formulation with an oil-based adjuvant

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