Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration

The development and validation of a simple and accurate method based on HPLC with ultraviolet detection for the quantification of zidovudine in rat plasma and its application to a pharmacokinetic study following a single intranasal dose zidovudine is described. Zidovudine was extracted from the plas...

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Autores principales:	MAINARDES,RUBIANA M, PALMIRA D. GREMIÃO,MARIA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	RP-HPLC validation AZT pharmacokinetics intranasal delivery
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300010
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spelling	oai:scielo:S0716-976020090003000102009-11-02Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administrationMAINARDES,RUBIANA MPALMIRA D. GREMIÃO,MARIA RP-HPLC validation AZT pharmacokinetics intranasal delivery The development and validation of a simple and accurate method based on HPLC with ultraviolet detection for the quantification of zidovudine in rat plasma and its application to a pharmacokinetic study following a single intranasal dose zidovudine is described. Zidovudine was extracted from the plasma using a single-step deproteinization. Chromatographic separation of zidovudine from interfering components was achieved with a C-18 reverse phase column, a mobile phase consisting of a mixture of sodium acetate buffer (55mM) with pH adjusted to 7.0 and acetonitrile (91:9 v/v) and UV detection set at 265 nm. The method was linear from 100 to 10000 ng.mL"¹ (r² > 0.9995), and zidovudine had a mean recovery from plasma of 92.8%. The coefficient of variation of inter-day and intra-day quality control samples was less than 15%. After a single intranasal dose of zidovudine administered to rats, pharmacokinetic parameters (AUC0 24, Cmax, t , t1/2) were determined. The proposed method was found to be simple, specific, accurate, and precise and could be applied to the quantitative analysis of clinical pharmacokinetic studies of zidovudine in rats.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.3 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300010en10.4067/S0716-97602009000300010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	RP-HPLC validation AZT pharmacokinetics intranasal delivery
spellingShingle	RP-HPLC validation AZT pharmacokinetics intranasal delivery MAINARDES,RUBIANA M PALMIRA D. GREMIÃO,MARIA Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration
description	The development and validation of a simple and accurate method based on HPLC with ultraviolet detection for the quantification of zidovudine in rat plasma and its application to a pharmacokinetic study following a single intranasal dose zidovudine is described. Zidovudine was extracted from the plasma using a single-step deproteinization. Chromatographic separation of zidovudine from interfering components was achieved with a C-18 reverse phase column, a mobile phase consisting of a mixture of sodium acetate buffer (55mM) with pH adjusted to 7.0 and acetonitrile (91:9 v/v) and UV detection set at 265 nm. The method was linear from 100 to 10000 ng.mL"¹ (r² > 0.9995), and zidovudine had a mean recovery from plasma of 92.8%. The coefficient of variation of inter-day and intra-day quality control samples was less than 15%. After a single intranasal dose of zidovudine administered to rats, pharmacokinetic parameters (AUC0 24, Cmax, t , t1/2) were determined. The proposed method was found to be simple, specific, accurate, and precise and could be applied to the quantitative analysis of clinical pharmacokinetic studies of zidovudine in rats.
author	MAINARDES,RUBIANA M PALMIRA D. GREMIÃO,MARIA
author_facet	MAINARDES,RUBIANA M PALMIRA D. GREMIÃO,MARIA
author_sort	MAINARDES,RUBIANA M
title	Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration
title_short	Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration
title_full	Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration
title_fullStr	Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration
title_full_unstemmed	Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration
title_sort	reversed phase hplc determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300010
work_keys_str_mv	AT mainardesrubianam reversedphasehplcdeterminationofzidovudineinratplasmaanditspharmacokineticsafterasingleintranasaldoseadministration AT palmiradgremiaomaria reversedphasehplcdeterminationofzidovudineinratplasmaanditspharmacokineticsafterasingleintranasaldoseadministration
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Reversed phase HPLC determination of zidovudine in rat plasma and its pharmacokinetics after a single intranasal dose administration

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