SHh activity and localization is regulated by perlecan

Proliferation and cell fate determination in the developing embryo are extrinsically regulated by multiple interactions among diverse secreted factors, such as Sonic Hedgehog (SHh), which act in a concentration-dependent manner. The fact that SHh is secreted as a lipid-modified protein suggests the...

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Autores principales:	Palma,Verónica, Carrasco,Héctor, Reinchisi,Gisela, Olivares,Gonzalo, Faunes,Fernando, Larraín,Juan
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2011
Materias:	SHh Morphogen gradients Perlecan
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100008
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spelling	oai:scielo:S0716-976020110001000082011-09-01SHh activity and localization is regulated by perlecanPalma,VerónicaCarrasco,HéctorReinchisi,GiselaOlivares,GonzaloFaunes,FernandoLarraín,Juan SHh Morphogen gradients Perlecan Proliferation and cell fate determination in the developing embryo are extrinsically regulated by multiple interactions among diverse secreted factors, such as Sonic Hedgehog (SHh), which act in a concentration-dependent manner. The fact that SHh is secreted as a lipid-modified protein suggests the existence of a mechanism to regulate its movement across embryonic fields. We have previously shown that heparan sulfate proteoglycans (HSPGs) are required for SHh binding and signalling. However, it was not determined which specific HSPG was responsible for these functions. Here we evaluated the contribution of perlecan on SHh localization and activity. To understand the mechanism of action of perlecan at the cellular level, we studied the role of perlecan-SHh interaction in SHh activity using both cell culture and biochemical assays. Our findings show that perlecan is a crucial anchor and modulator of SHh activity acting as an extracellular positive regulator of SHh.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.44 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100008en10.4067/S0716-97602011000100008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	SHh Morphogen gradients Perlecan
spellingShingle	SHh Morphogen gradients Perlecan Palma,Verónica Carrasco,Héctor Reinchisi,Gisela Olivares,Gonzalo Faunes,Fernando Larraín,Juan SHh activity and localization is regulated by perlecan
description	Proliferation and cell fate determination in the developing embryo are extrinsically regulated by multiple interactions among diverse secreted factors, such as Sonic Hedgehog (SHh), which act in a concentration-dependent manner. The fact that SHh is secreted as a lipid-modified protein suggests the existence of a mechanism to regulate its movement across embryonic fields. We have previously shown that heparan sulfate proteoglycans (HSPGs) are required for SHh binding and signalling. However, it was not determined which specific HSPG was responsible for these functions. Here we evaluated the contribution of perlecan on SHh localization and activity. To understand the mechanism of action of perlecan at the cellular level, we studied the role of perlecan-SHh interaction in SHh activity using both cell culture and biochemical assays. Our findings show that perlecan is a crucial anchor and modulator of SHh activity acting as an extracellular positive regulator of SHh.
author	Palma,Verónica Carrasco,Héctor Reinchisi,Gisela Olivares,Gonzalo Faunes,Fernando Larraín,Juan
author_facet	Palma,Verónica Carrasco,Héctor Reinchisi,Gisela Olivares,Gonzalo Faunes,Fernando Larraín,Juan
author_sort	Palma,Verónica
title	SHh activity and localization is regulated by perlecan
title_short	SHh activity and localization is regulated by perlecan
title_full	SHh activity and localization is regulated by perlecan
title_fullStr	SHh activity and localization is regulated by perlecan
title_full_unstemmed	SHh activity and localization is regulated by perlecan
title_sort	shh activity and localization is regulated by perlecan
publisher	Sociedad de Biología de Chile
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100008
work_keys_str_mv	AT palmaveronica shhactivityandlocalizationisregulatedbyperlecan AT carrascohector shhactivityandlocalizationisregulatedbyperlecan AT reinchisigisela shhactivityandlocalizationisregulatedbyperlecan AT olivaresgonzalo shhactivityandlocalizationisregulatedbyperlecan AT faunesfernando shhactivityandlocalizationisregulatedbyperlecan AT larrainjuan shhactivityandlocalizationisregulatedbyperlecan
_version_	1718441471472828416

SHh activity and localization is regulated by perlecan

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