Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses

Dendritic cells (DCs) are professional antigen-presenting cells involved in the control and initiation of immune responses. In vivo, DCs exposed at the periphery to maturation stimuli migrate to lymph nodes, where they receive secondary signals from CD4+ T helper cells. These DCs become able to init...

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Autores principales:	Simon,Thomas, Tanguy-Royer,Séverine, Royer,Pierre-Joseph, Boisgerault,Nicolas, Frikeche,Jihane, Fonteneau,Jean-François, Grégoire,Marc
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2012
Materias:	Cytotoxic T lymphocytes dendritic cells T memory cell sequential maturation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100005
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spelling	oai:scielo:S0716-976020120001000052012-05-15Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responsesSimon,ThomasTanguy-Royer,SéverineRoyer,Pierre-JosephBoisgerault,NicolasFrikeche,JihaneFonteneau,Jean-FrançoisGrégoire,Marc Cytotoxic T lymphocytes dendritic cells T memory cell sequential maturation Dendritic cells (DCs) are professional antigen-presenting cells involved in the control and initiation of immune responses. In vivo, DCs exposed at the periphery to maturation stimuli migrate to lymph nodes, where they receive secondary signals from CD4+ T helper cells. These DCs become able to initiate CD8+ cytotoxic T lymphocyte (CTL) responses. However, in vitro investigations concerning human monocyte-derived DCs have never focused on their functional properties after such sequential maturation. Here, we studied human DC phenotypes and functions according to this sequential exposure to maturation stimuli. As first signals, we used TNF-α/polyI:C mimicking inflammatory and pathogen stimuli and, as second signals, we compared activated CD4+ T helper cells to a combination of CD40-L/ IFN-γ. Our results show that a sequential activation with activated CD4+ T cells dramatically increased the maturation of DCs in terms of their phenotype and cytokine secretion compared to DCs activated with maturation stimuli delivered simultaneously. Furthermore, this sequential maturation led to the induction of CTL with a long-term effector and central memory phenotypes. Thus, sequential delivery of maturation stimuli, which includes CD4+ T cells, should be considered in the future to improve the induction of long-term CTL memory in DC-based immunotherapy.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.45 n.1 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100005en10.4067/S0716-97602012000100005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cytotoxic T lymphocytes dendritic cells T memory cell sequential maturation
spellingShingle	Cytotoxic T lymphocytes dendritic cells T memory cell sequential maturation Simon,Thomas Tanguy-Royer,Séverine Royer,Pierre-Joseph Boisgerault,Nicolas Frikeche,Jihane Fonteneau,Jean-François Grégoire,Marc Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses
description	Dendritic cells (DCs) are professional antigen-presenting cells involved in the control and initiation of immune responses. In vivo, DCs exposed at the periphery to maturation stimuli migrate to lymph nodes, where they receive secondary signals from CD4+ T helper cells. These DCs become able to initiate CD8+ cytotoxic T lymphocyte (CTL) responses. However, in vitro investigations concerning human monocyte-derived DCs have never focused on their functional properties after such sequential maturation. Here, we studied human DC phenotypes and functions according to this sequential exposure to maturation stimuli. As first signals, we used TNF-α/polyI:C mimicking inflammatory and pathogen stimuli and, as second signals, we compared activated CD4+ T helper cells to a combination of CD40-L/ IFN-γ. Our results show that a sequential activation with activated CD4+ T cells dramatically increased the maturation of DCs in terms of their phenotype and cytokine secretion compared to DCs activated with maturation stimuli delivered simultaneously. Furthermore, this sequential maturation led to the induction of CTL with a long-term effector and central memory phenotypes. Thus, sequential delivery of maturation stimuli, which includes CD4+ T cells, should be considered in the future to improve the induction of long-term CTL memory in DC-based immunotherapy.
author	Simon,Thomas Tanguy-Royer,Séverine Royer,Pierre-Joseph Boisgerault,Nicolas Frikeche,Jihane Fonteneau,Jean-François Grégoire,Marc
author_facet	Simon,Thomas Tanguy-Royer,Séverine Royer,Pierre-Joseph Boisgerault,Nicolas Frikeche,Jihane Fonteneau,Jean-François Grégoire,Marc
author_sort	Simon,Thomas
title	Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses
title_short	Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses
title_full	Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses
title_fullStr	Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses
title_full_unstemmed	Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses
title_sort	human dendritic cells sequentially matured with cd4+ t cells as a secondary signal favor ctl and long-term t memory cell responses
publisher	Sociedad de Biología de Chile
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100005
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Human dendritic cells sequentially matured with CD4+ T cells as a secondary signal favor CTL and long-term T memory cell responses

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