Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain

Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain

BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-β (β1, β5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-β in ost...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Zeng,Qiangcheng, Guo,Yong, Liu,Yongming, Li,Ruixin, Zhang,Xinchang, Liu,Lu, Wang,Yang, Zhang,Xizheng, Zou,Xianqiong
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2015
Materias:
Tensile strain
Osteoblast
Extracellular matrix
Integrin
siRNA
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:scielo:S0716-97602015000100025
record_format dspace
spelling oai:scielo:S0716-976020150001000252016-02-16Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strainZeng,QiangchengGuo,YongLiu,YongmingLi,RuixinZhang,XinchangLiu,LuWang,YangZhang,XizhengZou,Xianqiong Tensile strain Osteoblast Extracellular matrix Integrin siRNA BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-β (β1, β5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-β in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-β1 siRNA or integrin-β5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µε) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted osteoblastic differentiation of MC3T3-E1 cells. Transfection with integrin-β1 siRNA attenuated the osteoblastic diffenentiation induced by the tensile strain. By contrast, transfection with integrin-β5 siRNA had little effect on the osteoblastic differentiation induced by thestrain. At thesametime, theresultofECM formation promoted by the strain, was similar to the osteoblastic differentiation. CONCLUSION: Integrin-β1 mediates osteoblast differentiation and osteoblastic ECM formation promoted by cyclic tensile strain, and integrin-β5 is not involved in the osteoblasts response to the tensile strain.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.48 20152015-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025en10.1186/s40659-015-0014-y
institution Scielo Chile
collection Scielo Chile
language English
topic Tensile strain
Osteoblast
Extracellular matrix
Integrin
siRNA
spellingShingle Tensile strain
Osteoblast
Extracellular matrix
Integrin
siRNA
Zeng,Qiangcheng
Guo,Yong
Liu,Yongming
Li,Ruixin
Zhang,Xinchang
Liu,Lu
Wang,Yang
Zhang,Xizheng
Zou,Xianqiong
Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
description BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-β (β1, β5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-β in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-β1 siRNA or integrin-β5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µε) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted osteoblastic differentiation of MC3T3-E1 cells. Transfection with integrin-β1 siRNA attenuated the osteoblastic diffenentiation induced by the tensile strain. By contrast, transfection with integrin-β5 siRNA had little effect on the osteoblastic differentiation induced by thestrain. At thesametime, theresultofECM formation promoted by the strain, was similar to the osteoblastic differentiation. CONCLUSION: Integrin-β1 mediates osteoblast differentiation and osteoblastic ECM formation promoted by cyclic tensile strain, and integrin-β5 is not involved in the osteoblasts response to the tensile strain.
author Zeng,Qiangcheng
Guo,Yong
Liu,Yongming
Li,Ruixin
Zhang,Xinchang
Liu,Lu
Wang,Yang
Zhang,Xizheng
Zou,Xianqiong
author_facet Zeng,Qiangcheng
Guo,Yong
Liu,Yongming
Li,Ruixin
Zhang,Xinchang
Liu,Lu
Wang,Yang
Zhang,Xizheng
Zou,Xianqiong
author_sort Zeng,Qiangcheng
title Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
title_short Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
title_full Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
title_fullStr Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
title_full_unstemmed Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain
title_sort integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ecm formation promoted by mechanical tensile strain
publisher Sociedad de Biología de Chile
publishDate 2015
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100025
work_keys_str_mv AT zengqiangcheng integrin9461notintegrin9465mediatesosteoblasticdifferentiationandecmformationpromotedbymechanicaltensilestrain
AT guoyong integrin9461notintegrin9465mediatesosteoblasticdifferentiationandecmformationpromotedbymechanicaltensilestrain
AT liuyongming integrin9461notintegrin9465mediatesosteoblasticdifferentiationandecmformationpromotedbymechanicaltensilestrain
AT liruixin integrin9461notintegrin9465mediatesosteoblasticdifferentiationandecmformationpromotedbymechanicaltensilestrain
AT zhangxinchang integrin9461notintegrin9465mediatesosteoblasticdifferentiationandecmformationpromotedbymechanicaltensilestrain
AT liulu integrin9461notintegrin9465mediatesosteoblasticdifferentiationandecmformationpromotedbymechanicaltensilestrain
AT wangyang integrin9461notintegrin9465mediatesosteoblasticdifferentiationandecmformationpromotedbymechanicaltensilestrain
AT zhangxizheng integrin9461notintegrin9465mediatesosteoblasticdifferentiationandecmformationpromotedbymechanicaltensilestrain
AT zouxianqiong integrin9461notintegrin9465mediatesosteoblasticdifferentiationandecmformationpromotedbymechanicaltensilestrain
_version_ 1718441533539090432

Ejemplares similares