Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model

BACKGROUND: Aged garlic extract (AGE) and its main constituent S-allylcysteine (SAC) are natural antioxidants with protective effects against cerebral ischemia or cancer, events that involve hypoxia stress. Cobalt chloride (CoCl2) has been used to mimic hypoxic conditions through the stabilization o...

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Autores principales:	Orozco-Ibarra,Marisol, Muñoz-Sánchez,Jorge, Zavala-Medina,Martín E, Pineda,Benjamín, Magaña-Maldonado,Roxana, Vázquez-Contreras,Edgar, Maldonado,Perla D, Pedraza-Chaverri,José, Chánez-Cárdenas,María Elena
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2016
Materias:	Aged garlic extract Chemical hypoxia Cobalt chloride Hypoxia-inducible factor 1 PC12 cells S-allylcysteine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100007
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spelling	oai:scielo:S0716-976020160001000072016-12-01Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia modelOrozco-Ibarra,MarisolMuñoz-Sánchez,JorgeZavala-Medina,Martín EPineda,BenjamínMagaña-Maldonado,RoxanaVázquez-Contreras,EdgarMaldonado,Perla DPedraza-Chaverri,JoséChánez-Cárdenas,María Elena Aged garlic extract Chemical hypoxia Cobalt chloride Hypoxia-inducible factor 1 PC12 cells S-allylcysteine BACKGROUND: Aged garlic extract (AGE) and its main constituent S-allylcysteine (SAC) are natural antioxidants with protective effects against cerebral ischemia or cancer, events that involve hypoxia stress. Cobalt chloride (CoCl2) has been used to mimic hypoxic conditions through the stabilization of the α subunit of hypoxia inducible factor (HIF-Ια) and up-regulation of HIF-1a-dependent genes as well as activation of hypoxic conditions such as reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and apoptosis. The present study was designed to assess the effect of AGE and SAC on the CoCl2-chemical hypoxia model in PC12 cells RESULTS: We found that CoCl2 induced the stabilization of HIF-1a and its nuclear localization. CoCl2 produced ROS and apoptotic cell death that depended on hypoxia extent. The treatment with AGE and SAC decreased ROS and protected against CoCl2-induced apoptotic cell death which depended on the CoCl2 concentration and incubation time. SAC or AGE decreased the number of cells in the early and late stages of apoptosis. Interestingly, this protective effect was associated with attenuation in HIF-1a stabilization, activity not previously reported for AGE and SAC CONCLUSIONS: Obtained results show that AGE and SAC decreased apoptotic CoCl2-induced cell death. This protection occurs by affecting the activity of HIF-1a and supports the use of these natural compounds as a therapeutic alternative for hypoxic conditionsinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.49 20162016-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100007en10.1186/s40659-016-0067-6
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aged garlic extract Chemical hypoxia Cobalt chloride Hypoxia-inducible factor 1 PC12 cells S-allylcysteine
spellingShingle	Aged garlic extract Chemical hypoxia Cobalt chloride Hypoxia-inducible factor 1 PC12 cells S-allylcysteine Orozco-Ibarra,Marisol Muñoz-Sánchez,Jorge Zavala-Medina,Martín E Pineda,Benjamín Magaña-Maldonado,Roxana Vázquez-Contreras,Edgar Maldonado,Perla D Pedraza-Chaverri,José Chánez-Cárdenas,María Elena Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model
description	BACKGROUND: Aged garlic extract (AGE) and its main constituent S-allylcysteine (SAC) are natural antioxidants with protective effects against cerebral ischemia or cancer, events that involve hypoxia stress. Cobalt chloride (CoCl2) has been used to mimic hypoxic conditions through the stabilization of the α subunit of hypoxia inducible factor (HIF-Ια) and up-regulation of HIF-1a-dependent genes as well as activation of hypoxic conditions such as reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential and apoptosis. The present study was designed to assess the effect of AGE and SAC on the CoCl2-chemical hypoxia model in PC12 cells RESULTS: We found that CoCl2 induced the stabilization of HIF-1a and its nuclear localization. CoCl2 produced ROS and apoptotic cell death that depended on hypoxia extent. The treatment with AGE and SAC decreased ROS and protected against CoCl2-induced apoptotic cell death which depended on the CoCl2 concentration and incubation time. SAC or AGE decreased the number of cells in the early and late stages of apoptosis. Interestingly, this protective effect was associated with attenuation in HIF-1a stabilization, activity not previously reported for AGE and SAC CONCLUSIONS: Obtained results show that AGE and SAC decreased apoptotic CoCl2-induced cell death. This protection occurs by affecting the activity of HIF-1a and supports the use of these natural compounds as a therapeutic alternative for hypoxic conditions
author	Orozco-Ibarra,Marisol Muñoz-Sánchez,Jorge Zavala-Medina,Martín E Pineda,Benjamín Magaña-Maldonado,Roxana Vázquez-Contreras,Edgar Maldonado,Perla D Pedraza-Chaverri,José Chánez-Cárdenas,María Elena
author_facet	Orozco-Ibarra,Marisol Muñoz-Sánchez,Jorge Zavala-Medina,Martín E Pineda,Benjamín Magaña-Maldonado,Roxana Vázquez-Contreras,Edgar Maldonado,Perla D Pedraza-Chaverri,José Chánez-Cárdenas,María Elena
author_sort	Orozco-Ibarra,Marisol
title	Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model
title_short	Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model
title_full	Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model
title_fullStr	Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model
title_full_unstemmed	Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model
title_sort	aged garlic extract and s-allylcysteine prevent apoptotic cell death in a chemical hypoxia model
publisher	Sociedad de Biología de Chile
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100007
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Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model

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