BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma

Abstract Background: Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC). Methods:...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang,Limei, Wu,Xiuyin, Wang,Ruolin, Yang,Chengzhe, Li,Zhi, Wang,Cunwei, Zhang,Fenghe, Yang,Pishan
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2017
Materias:	Salivary adenoid cystic carcinoma BRD4 inhibition Proliferation Migration Epithelial-mesenchymal transition
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100213
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602017000100213
record_format	dspace
spelling	oai:scielo:S0716-976020170001002132017-07-07BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinomaWang,LimeiWu,XiuyinWang,RuolinYang,ChengzheLi,ZhiWang,CunweiZhang,FengheYang,Pishan Salivary adenoid cystic carcinoma BRD4 inhibition Proliferation Migration Epithelial-mesenchymal transition Abstract Background: Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC). Methods: The human normal epithelial cells and SACC cells (ACC-LM and ACC-83) were treated with JQ1 at concentrations of 0, 0.1, 0.5 or 1 μM. Cell Counting Kit-8 (CCK-8) assay was performed to evaluate cell proliferation. Cell apoptosis and cell cycle distribution was evaluated by Flow cytometry. Immunofluorescence staining was used to examine the expression of BRD4 in SACC cells. The quantitative real-time polymerase chain reaction (qRT-PCR) assay and western blot assay were performed to examine messenger RNA (mRNA) and protein levels in SACC cells. Wound- healing assay and transwell assay were used to evaluate the activities of migration and invasion of SACC cells. Results: JQ1 exhibits no adverse effects on proliferation, cell cycle and cell apoptosis of the normal human epithelial cells, while suppressed proliferation and cell cycle, and induced apoptosis of SACC cells, down-regulated the mRNA and protein levels of BRD4 in SACC cells, meanwhile reduced protein expressions of c-myc and BCL-2, two known target genes of BRD4. Moreover, JQ1 inhibited SACC cell migration and invasion by regulating key epithelial-mesenchymal transition (EMT) characteristics including E-cadherin, Vimentin and Twist. Conclusions: BRD4 is an important transcription factor in SACC and BRD4 inhibition by JQ1 may be a new strategy for SACC treatment.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.50 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100213en10.1186/s40659-017-0124-9
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Salivary adenoid cystic carcinoma BRD4 inhibition Proliferation Migration Epithelial-mesenchymal transition
spellingShingle	Salivary adenoid cystic carcinoma BRD4 inhibition Proliferation Migration Epithelial-mesenchymal transition Wang,Limei Wu,Xiuyin Wang,Ruolin Yang,Chengzhe Li,Zhi Wang,Cunwei Zhang,Fenghe Yang,Pishan BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma
description	Abstract Background: Bromodomain-containing protein 4 (BRD4) inhibition is a new therapeutic strategy for many malignancies. In this study, we aimed to explore the effect of BRD4 inhibition by JQ1 on in vitro cell growth, migration and invasion of salivary adenoid cystic carcinoma (SACC). Methods: The human normal epithelial cells and SACC cells (ACC-LM and ACC-83) were treated with JQ1 at concentrations of 0, 0.1, 0.5 or 1 μM. Cell Counting Kit-8 (CCK-8) assay was performed to evaluate cell proliferation. Cell apoptosis and cell cycle distribution was evaluated by Flow cytometry. Immunofluorescence staining was used to examine the expression of BRD4 in SACC cells. The quantitative real-time polymerase chain reaction (qRT-PCR) assay and western blot assay were performed to examine messenger RNA (mRNA) and protein levels in SACC cells. Wound- healing assay and transwell assay were used to evaluate the activities of migration and invasion of SACC cells. Results: JQ1 exhibits no adverse effects on proliferation, cell cycle and cell apoptosis of the normal human epithelial cells, while suppressed proliferation and cell cycle, and induced apoptosis of SACC cells, down-regulated the mRNA and protein levels of BRD4 in SACC cells, meanwhile reduced protein expressions of c-myc and BCL-2, two known target genes of BRD4. Moreover, JQ1 inhibited SACC cell migration and invasion by regulating key epithelial-mesenchymal transition (EMT) characteristics including E-cadherin, Vimentin and Twist. Conclusions: BRD4 is an important transcription factor in SACC and BRD4 inhibition by JQ1 may be a new strategy for SACC treatment.
author	Wang,Limei Wu,Xiuyin Wang,Ruolin Yang,Chengzhe Li,Zhi Wang,Cunwei Zhang,Fenghe Yang,Pishan
author_facet	Wang,Limei Wu,Xiuyin Wang,Ruolin Yang,Chengzhe Li,Zhi Wang,Cunwei Zhang,Fenghe Yang,Pishan
author_sort	Wang,Limei
title	BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma
title_short	BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma
title_full	BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma
title_fullStr	BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma
title_full_unstemmed	BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma
title_sort	brd4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma
publisher	Sociedad de Biología de Chile
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100213
work_keys_str_mv	AT wanglimei brd4inhibitionsuppressescellgrowthmigrationandinvasionofsalivaryadenoidcysticcarcinoma AT wuxiuyin brd4inhibitionsuppressescellgrowthmigrationandinvasionofsalivaryadenoidcysticcarcinoma AT wangruolin brd4inhibitionsuppressescellgrowthmigrationandinvasionofsalivaryadenoidcysticcarcinoma AT yangchengzhe brd4inhibitionsuppressescellgrowthmigrationandinvasionofsalivaryadenoidcysticcarcinoma AT lizhi brd4inhibitionsuppressescellgrowthmigrationandinvasionofsalivaryadenoidcysticcarcinoma AT wangcunwei brd4inhibitionsuppressescellgrowthmigrationandinvasionofsalivaryadenoidcysticcarcinoma AT zhangfenghe brd4inhibitionsuppressescellgrowthmigrationandinvasionofsalivaryadenoidcysticcarcinoma AT yangpishan brd4inhibitionsuppressescellgrowthmigrationandinvasionofsalivaryadenoidcysticcarcinoma
_version_	1718441561474203648

BRD4 inhibition suppresses cell growth, migration and invasion of salivary adenoid cystic carcinoma

Ejemplares similares